LOCUS       sequence-282413-        1934 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1934)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1934)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0668
FEATURES             Location/Qualifiers
     source          1..1934
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggactcagt ggcctttgaa gatgtggctg tgaacttcac acaagaggag
       61 tgggctttgc tgggtccatc acagaagagt ctctacagaa atgtcatgca ggaaaccatt
      121 aggaacctgg actgtataga aatgaaatgg gaggaccaga acattggaga tcagtgccaa
      181 aatgccaaga gaaatctaag aagtcataca tgtgaaatta aagatgacag tcaatgtgga
      241 gaaacttttg gccagattcc agatagtatt gtgaacaaga acactcctcg agtaaatcca
      301 tgtgacagtg gtgagtgtgg agaagtcgtc ttgggtcatt cgtctcttaa ttgcaacatc
      361 agagttgaca ctggacacaa atcatgtgag catcaggaat atggagagaa gccatataca
      421 cataaacaac gtgggaaagc catcagtcat cagcactcct tccagacaca tgaaaggccc
      481 cccaccggaa agaaaccctt cgattgtaaa gaatgtgcaa aaacctttag ttctcttgga
      541 aacctccgaa gacacatggc ggcacaccat ggagatggac cttataaatg taagttgtgt
      601 gggaaagcct ttgtttggcc cagtttattt catttgcacg aaagaacaca cactggagag
      661 aaaccgtatg aatgtaagca gtgttctaaa gcctttcctt tttacagttc ctatctaaga
      721 catgaaagaa tccacacggg agagaaagcg tatgaatgta agcagtgttc caaagccttt
      781 cctgattaca gtacctatct aagacatgag agaactcaca ccggagagaa accctataaa
      841 tgtacacaat gtgggaaagc cttcagctgt tactattaca ctcgactaca tgaaaggact
      901 cacacgggag aacaacccta tgcatgtaag caatgtggga aaacgtttta tcatcacaca
      961 agctttcgaa gacacatgat aaggcacact ggagacggac cacataaatg taagatatgt
     1021 gggaaaggct ttgattgtcc tagttcagtt cgaaatcatg aaactactca cactggagag
     1081 aaaccctatg aatgtaagca gtgtgggaaa gtgttatctc atagctcgag ctttcgaagt
     1141 cacatgataa cacacacagg agatggaccc cagaaatgca agatatgtgg gaaagccttt
     1201 ggttgtccca gtttatttca aagacatgaa aggactcaca ctggagagaa accctatcaa
     1261 tgtaaacaat gtggtaaagc cttcagtctt gccggttccc ttcgaagaca tgaagcaact
     1321 cacactggag tgaaacccta taaatgtcag tgtgggaaag cctttagtga tctctcttcc
     1381 tttcaaaatc atgagacaac tcacactgga gagaagccat atgagtgtaa ggaatgtggg
     1441 aaagcattca gttgtttcaa atacctttct caacataaaa ggacccacac agtagaaaaa
     1501 ccttatgagt gtaaaacatg tagaaaagcc ttcagtcatt tcagtaactt aaaagtccat
     1561 gaaaggattc actctggaga gaagccatat gaatgtaagg aatgtggaaa agcattctct
     1621 tggctcactt gccttctacg acatgaaaga attcacactg gagagaaacc ctatgaatgt
     1681 ctacaatgtg gtaaagcctt cactcgttcc cgtttccttc gaggacatga aaaaactcac
     1741 actggagaga agctgtatga atgtaaggaa tgtgggaaag cattgagttc tctccgttcc
     1801 ttgcatagac ataaaaggac tcactggaaa gatactctct agtaatgaac tagtccgtaa
     1861 cttgaaagta tttcgatttc ttggctttat atatcttgtg gaaaggacga ggacgtcgta
     1921 gttaacaact ctca
//
