LOCUS       sequence-282416-        3698 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3698)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 3698)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0679
FEATURES             Location/Qualifiers
     source          1..3698
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(595..606)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 accgccacca tgccccgccg ccgccccccg gcttccggtg ctgcgcagtt tccggagcgg
       61 atcgcaaccc ggagtccgga tccgatcccg ctctgcacat tccaaaggca ggtgtcagag
      121 atggctgaag atgggagcga agagatcatg ttcatctggt gtgaagactg cagccagtac
      181 cacgactccg aatgtcccga gctgggccca gtggtcatgg tcaaagactc ctttgtgtta
      241 agcagggcaa ggtcatccct tcctcccaac ttggagatca gacgactgga agatggagcc
      301 gagggggtgt tcgccatcac tcagctcgtc aagcggacac agttcggtcc ctttgagtcc
      361 aggagggtcg ccaaatggga aaaggagtct gcatttcccc tgaaggtgtt ccagaaggac
      421 gggcaccccg tgtgcttcga cacctccaac gaggatgact gcaactggat gatgctggtg
      481 cggccagcgg cggaggccga gcaccagaac ctgacggcct accagcacgg cagcgacgtg
      541 tacttcacca cctccagaga catccccccg ggtaccgagc tgcgcgtgtg gtatgcggcc
      601 ttctatgcca agaagatgga caagcccatg ctgaagcagg ccggctctgg cgtccacgct
      661 gcaggcaccc cagaaaacag cgcccccgtg gagtcggagc ccagccagtg ggcgtgtaaa
      721 gtgtgttctg ccaccttcct ggagctgcag ctcctcaatg aacatctgtt gggccactta
      781 gaacaagcca aaagccttcc tccaggcagc caaagcgagg cagcagctcc cgagaaggag
      841 caggacacac cccgggggga accccctgca gtgcccgaga gcgagaatgt tgccaccaaa
      901 gaacagaaga aaaagcctcg aagggggaga aaacccaaag tgtccaaagc tgagcagcct
      961 ctagtcatcg tggaagacaa ggaacccaca gagcaagtgg cagagatcat taccgaggtc
     1021 cctccggatg agcctgtgag tgcaacgcca gatgagcgga tcatggagct ggttctgggg
     1081 aagctggcca ccaccaccac tgacaccagc tcggttccaa agttcaccca tcatcagaat
     1141 aacaccatca cgctcaagag gagcttaatt ctctcaagca gacacggcat ccggcgcaag
     1201 ctcatcaaac agctcgggga gcacaagcgg gtttaccagt gcaatatctg cagcaagatc
     1261 ttccagaaca gcagcaacct gagcaggcac gtgcgctcgc atggtgacaa gctgtttaag
     1321 tgcgaagagt gtgcaaaatt gttcagccgc aaagagagcc taaagcagca cgtttcctac
     1381 aagcacagca ggaacgaggt ggacggcgag tacaggtacc gctgcggcac ttgtgagaag
     1441 accttccgca tcgagagcgc gctggagttc cacaactgca ggacaggact catagcacac
     1501 cctggagagg gggggcccgg cggcagtcgg cttagagacc taccagatga caagacgttc
     1561 caatgtgaga tgtgtttcag attcttctcc accaacagca acctctccaa gcacaagaag
     1621 aagcacggcg acaagaagtt tgcctgtgag gtctgcagca agatgttcta ccgcaaggac
     1681 gtcatgctgg accaccagcg ccggcacctg gaaggagtgc ggcgagtgaa gcgagaggac
     1741 ctggaggccg gtggggagaa cctggtccgt tacaagaagg agccttccgg gtgcccggtg
     1801 tgtggcaagg tgttctcctg ccggagcaat atgaacaagc acctgctcac ccacggcgac
     1861 aagaagtaca cctgcgagat ctgcgggcgc aagttcttcc gcgtggatgt gctcagggac
     1921 cacatccatg tccacttcaa ggacatcgcg ttgatggatg accaccagag ggaagagttt
     1981 atcggcaaga tcgggatctc ctcggaagaa aacgatgaca attctgacga gagcgcagac
     2041 tcggagcctc acaagtacag ctgcaagcgg tgccagctca ccttcggccg ggggaaggag
     2101 tacctgaagc acatcatgga ggtgcacaag gagaagggct atggctgcag catctgcaac
     2161 cggcgctttg cactgaaggc cacctaccac gcccacatgg tcatccaccg tgaaaacctg
     2221 ccggacccca acgtgcagaa gtacatccac ccctgcgaga tctgcgggcg gatcttcaac
     2281 agcatcggga acctggagcg ccacaagctc atccacacag gtgtgaagag ccacgcctgc
     2341 gagcagtgtg ggaagtcctt tgccaggaag gacatgctga aggagcacat gcgtgtgcac
     2401 gacaatgtcc gcgagtacct gtgtgccgag tgtgggaaag gcatgaagac caagcacgcg
     2461 ctgcgccacc acatgaagct gcacaagggc atcaaggagt acgagtgcaa ggagtgccac
     2521 cgcaggttcg cgcagaaggt caacatgctc aagcactgca agcggcacac ggggattaaa
     2581 gatttcatgt gtgaattgtg tgggaagaca ttcagcgaga ggaacaccat ggagacccac
     2641 aagctcatcc acacagtggg caagcagtgg acgtgctccg tgtgcgacaa gaagtacgtg
     2701 accgagtaca tgctgcagaa gcacgttcag ctcacacacg acaaggtgga ggcgcagagc
     2761 tgccagctgt gcgggaccaa ggtgtccacc agggcctcca tgagccgaca catgcggcgc
     2821 aagcaccccg aggtgctcgc ggtgaggatc gatgacctgg accacctccc ggagaccacc
     2881 accatcgacg cctcctccat tggcatcgtc cagcctgagc tgactctgga gcaggaggat
     2941 ttggccgaag ggaagcacgg gaaagctgcc aagcgaagtc acaagagaaa gcagaagcca
     3001 gaagaggagg cgggtgctcc ggtgcccgag gacgccacct tcagcgaata ctcagagaaa
     3061 gagacggagt tcacaggcag tgtaggcgac gagaccaatt ccgcagtaca gagcattcag
     3121 caggtagtgg tgaccctggg tgacccaaat gtgaccacac catcgagctc agtcggctta
     3181 accaacatca ccgtgacccc catcaccact gcggccgcga ctcagtttac caatctccag
     3241 ccggtggccg tggggcacct taccacccct gaacgccagt tacagctgga caactcaatc
     3301 ctgaccgtga cctttgatac cgtcagcggc tctgccatgt tgcacaaccg ccaaaatgac
     3361 gtccagatcc acccccagcc ggaagcctcg aacccacagt ctgtggccca tttcatcaac
     3421 ctgacgaccc tggtcaactc catcacgccc ctggggagcc agcttagtga ccagcacccg
     3481 ctcacgtggc gggcagtgcc ccagactgac gtcttgccac cctcgcagcc gcaggcaccc
     3541 ccacagcagg cggcccagcc ccaggtgcag gcggagcagc agcagcagca gatgtacagc
     3601 tactagtaat gaactagtcc gtaacttgaa agtatttcga tttcttggct ttatatatct
     3661 tgtggaaagg acgaaacgcg atcatcctgt aagcctaa
//
