LOCUS       sequence-282419-        3638 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3638)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 3638)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0680
FEATURES             Location/Qualifiers
     source          1..3638
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(595..606)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 accgccacca tgccccgccg ccgccccccg gcttccggtg ctgcgcagtt tccggagcgg
       61 atcgcaaccc ggagtccgga tccgatcccg ctctgcacat tccaaaggca ggtgtcagag
      121 atggctgaag atgggagcga agagatcatg ttcatctggt gtgaagactg cagccagtac
      181 cacgactccg aatgtcccga gctgggccca gtggtcatgg tcaaagactc ctttgtgtta
      241 agcagggcaa ggtcatccct tcctcccaac ttggagatca gacgactgga agatggagcc
      301 gagggggtgt tcgccatcac tcagctcgtc aagcggacac agttcggtcc ctttgagtcc
      361 aggagggtcg ccaaatggga aaaggagtct gcatttcccc tgaaggtgtt ccagaaggac
      421 gggcaccccg tgtgcttcga cacctccaac gaggatgact gcaactggat gatgctggtg
      481 cggccagcgg cggaggccga gcaccagaac ctgacggcct accagcacgg cagcgacgtg
      541 tacttcacca cctccagaga catccccccg ggtaccgagc tgcgcgtgtg gtatgcggcc
      601 ttctatgcca agaagatgga caagcccatg ctgaagcagg ccggctctgg cgtccacgct
      661 gcaggcaccc cagaaaacag cgcccccgtg gagtcggagc ccagccagtg ggcgtgtaaa
      721 gtgtgttctg ccaccttcct ggagctgcag ctcctcaatg aacatctgtt gggccactta
      781 gaacaagcca aaagccttcc tccaggcagc caaagcgagg cagcagctcc cgagaaggag
      841 caggacacac cccgggggga accccctgca gtgcccgaga gcgagaatgt tgccaccaaa
      901 gaacagaaga aaaagcctcg aagggggaga aaacccaaag tgtccaaagc tgagcagcct
      961 ctagtcatcg tggaagacaa ggaacccaca gagcaagtgg cagagatcat taccgaggtc
     1021 cctccggatg agcctgtgag tgcaacgcca gatgagcgga tcatggagct ggttctgggg
     1081 aagctggcca ccaccaccac tgacaccagc tcggttccaa agttcaccca tcatcagaat
     1141 aacaccatca cgctcaagag gagcttaatt ctctcaagca gacacggcat ccggcgcaag
     1201 ctcatcaaac agctcgggga gcacaagcgg gtttaccagt gcaatatctg cagcaagatc
     1261 ttccagaaca gcagcaacct gagcaggcac gtgcgctcgc atggtgacaa gctgtttaag
     1321 tgcgaagagt gtgcaaaatt gttcagccgc aaagagagcc taaagcagca cgtttcctac
     1381 aagcacagca ggaacgaggt ggacggcgag tacaggtacc gctgcggcac ttgtgagaag
     1441 accttccgca tcgagagcgc gctggagttc cacaactgca ggacagatga caagacgttc
     1501 caatgtgaga tgtgtttcag attcttctcc accaacagca acctctccaa gcacaagaag
     1561 aagcacggcg acaagaagtt tgcctgtgag gtctgcagca agatgttcta ccgcaaggac
     1621 gtcatgctgg accaccagcg ccggcacctg gaaggagtgc ggcgagtgaa gcgagaggac
     1681 ctggaggccg gtggggagaa cctggtccgt tacaagaagg agccttccgg gtgcccggtg
     1741 tgtggcaagg tgttctcctg ccggagcaat atgaacaagc acctgctcac ccacggcgac
     1801 aagaagtaca cctgcgagat ctgcgggcgc aagttcttcc gcgtggatgt gctcagggac
     1861 cacatccatg tccacttcaa ggacatcgcg ttgatggatg accaccagag ggaagagttt
     1921 atcggcaaga tcgggatctc ctcggaagaa aacgatgaca attctgacga gagcgcagac
     1981 tcggagcctc acaagtacag ctgcaagcgg tgccagctca ccttcggccg ggggaaggag
     2041 tacctgaagc acatcatgga ggtgcacaag gagaagggct atggctgcag catctgcaac
     2101 cggcgctttg cactgaaggc cacctaccac gcccacatgg tcatccaccg tgaaaacctg
     2161 ccggacccca acgtgcagaa gtacatccac ccctgcgaga tctgcgggcg gatcttcaac
     2221 agcatcggga acctggagcg ccacaagctc atccacacag gtgtgaagag ccacgcctgc
     2281 gagcagtgtg ggaagtcctt tgccaggaag gacatgctga aggagcacat gcgtgtgcac
     2341 gacaatgtcc gcgagtacct gtgtgccgag tgtgggaaag gcatgaagac caagcacgcg
     2401 ctgcgccacc acatgaagct gcacaagggc atcaaggagt acgagtgcaa ggagtgccac
     2461 cgcaggttcg cgcagaaggt caacatgctc aagcactgca agcggcacac ggggattaaa
     2521 gatttcatgt gtgaattgtg tgggaagaca ttcagcgaga ggaacaccat ggagacccac
     2581 aagctcatcc acacagtggg caagcagtgg acgtgctccg tgtgcgacaa gaagtacgtg
     2641 accgagtaca tgctgcagaa gcacgttcag ctcacacacg acaaggtgga ggcgcagagc
     2701 tgccagctgt gcgggaccaa ggtgtccacc agggcctcca tgagccgaca catgcggcgc
     2761 aagcaccccg aggtgctcgc ggtgaggatc gatgacctgg accacctccc ggagaccacc
     2821 accatcgacg cctcctccat tggcatcgtc cagcctgagc tgactctgga gcaggaggat
     2881 ttggccgaag ggaagcacgg gaaagctgcc aagcgaagtc acaagagaaa gcagaagcca
     2941 gaagaggagg cgggtgctcc ggtgcccgag gacgccacct tcagcgaata ctcagagaaa
     3001 gagacggagt tcacaggcag tgtaggcgac gagaccaatt ccgcagtaca gagcattcag
     3061 caggtagtgg tgaccctggg tgacccaaat gtgaccacac catcgagctc agtcggctta
     3121 accaacatca ccgtgacccc catcaccact gcggccgcga ctcagtttac caatctccag
     3181 ccggtggccg tggggcacct taccacccct gaacgccagt tacagctgga caactcaatc
     3241 ctgaccgtga cctttgatac cgtcagcggc tctgccatgt tgcacaaccg ccaaaatgac
     3301 gtccagatcc acccccagcc ggaagcctcg aacccacagt ctgtggccca tttcatcaac
     3361 ctgacgaccc tggtcaactc catcacgccc ctggggagcc agcttagtga ccagcacccg
     3421 ctcacgtggc gggcagtgcc ccagactgac gtcttgccac cctcgcagcc gcaggcaccc
     3481 ccacagcagg cggcccagcc ccaggtgcag gcggagcagc agcagcagca gatgtacagc
     3541 tactagtaat gaactagtcc gtaacttgaa agtatttcga tttcttggct ttatatatct
     3601 tgtggaaagg acgaagaacc atgaagcgga tttactga
//
