LOCUS       sequence-28242-e         752 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 752)
  AUTHORS   Hanaoka K, Qian F, Boletta A, Bhunia AK, Piontek K, Tsiokas L, 
            Sukhatme VP, Guggino WB, Germino GG
  TITLE     Co-assembly of polycystin-1 and -2 produces unique cation-permeable 
            currents.
  JOURNAL   Nature.   . 408(6815):990-4.
  PUBMED    11140688
REFERENCE   2  (bases 1 to 752)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature.   .
            408(6815):990-4."
COMMENT     Sequence Label: R4227X-F
FEATURES             Location/Qualifiers
     source          1..752
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             247..270
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     polyA_signal    378..499
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(389..408)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     443..462
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 gtcagggagt tccgccacan agtccgcttt gaagggatgg agccgctgcc ctctcgctcc
       61 tccaggggct ccaaggtatc cccggatgtg cccccaccca gcgctggctc cgatgcctcg
      121 cacccctcca cctcctccag ccagctggat gggctgagcg tgagcctggg ccggctgggg
      181 acaaggtgtg agcctgagcc ctcccgcctc caagccgtgt tcgaggccct gctcacccag
      241 tttgacgact acaaggacga cgatgacaag tagaagcttt taagccgaat tccagcacac
      301 tggcggccgt tactagtgga tccgagctcg gtacctctag agtcgacccg ggcggccgcc
      361 gcttcgagca gacatgataa gatacattga tgagtttgga caaaccacaa ctagaatgca
      421 gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt gctttatttg taaccattat
      481 aagctgcaat aaacaagtta acaacaacaa ttgcattcat tttatgtttc aggttcaggg
      541 ggagatgtgg gaggtttttt aaagcaagta aaacctctac aaatgtggta aaatcgataa
      601 ggatcaattg cctttttttt taatttttat tttattttat ttttgagatg gccagatccg
      661 ggctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagnctg
      721 aatggcgaat ggacgcnncc tgtagcggcg ca
//
