LOCUS       sequence-282428-        3572 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3572)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 3572)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0687
FEATURES             Location/Qualifiers
     source          1..3572
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3420..3437
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 accgccacca tggattccaa agatgaaagc tcgcatgtgt ggccgacatc tgcagagcat
       61 gaacagaatg ccgcacaggt gcactttgtt ccggacacag gaacagtggc tcagattgtc
      121 tataccgatg accaggttcg ccccccacag caggtggtgt acacggcaga tggtgcctcc
      181 tacacatcag tggatggtcc agagcacacg ctggtgtaca tccacccggt ggaagctgca
      241 cagactctgt ttacagaccc cggccaggta gcttatgtcc aacaggatgc tacagctcag
      301 caggcctcat tgccagttca taaccaggtg ctgccttcca tcgagagtgt agatgggtcc
      361 gaccctttgg caactctgca gacccctcta ggcagactgg aggccaaaga ggaagaggat
      421 gaggatgagg acgaggacac tgaggaagat gaggaagaag acggtgagga cacggatctg
      481 gatgactggg agccagaccc gccccggccc ttcgacccac acgacttgtg gtgtgaggag
      541 tgcaataacg cgcatgcttc agtgtgtccg aagcacggcc ccttgcaccc gatccccaac
      601 cggccggtgc tcacccgggc cagggcgagc ctccccctgg tgctctacat agacaggttt
      661 ctgggcgggg tgttctccaa gcggcgcatc cccaagcgca cccagtttgg ccccgtggag
      721 gggcctctcg tcaggggctc ggagctgaaa gactgttaca ttcacctcaa ggtttctctt
      781 gataaagggg acaggaaaga aagggattta catgaagacc tatggtttga gttgtctgat
      841 gagacgcttt gtaactggat gatgtttgta cggccagccc agaatcacct ggagcagaac
      901 ctggtggctt accagtatgg ccaccatgtg tattatacaa ccataaaaaa tgtggagccc
      961 aagcaggaac tgaaggtgtg gtatgccgca tcctatgctg agttcgtgaa ccagaaaatt
     1021 catgacattt ctgaggaaga aaggaaagtt cttcgagagc aagagaagaa ttggccctgc
     1081 tatgaatgta accgccgatt tataagctcg gagcagttgc aacagcatct caattctcat
     1141 gatgagaaac tagatgtgtt tagcagaaca agaggcagag gaaggggacg aggcaagagg
     1201 cgattcggtc caggtcgacg gccggggcgt cctccaaaat ttatccgcct ggaaatcacc
     1261 agcgaaaatg gggaaaagag tgacgatggg acacaggact tgctacattt tcccacaaag
     1321 gagcaatttg atgaggctga accagccact ctgaatgggc tggatcaacc agaacagacc
     1381 actatcccaa tccctcagct gccacaggaa acccagtctt ccctggaaca tgaaccagaa
     1441 actcacaccc tgcacctgca gccgcagcat gaagagagcg tggtgcccac ccagagcacg
     1501 ctgacagccg acgacatgcg cagagccaag cgcatccgaa atgcagctct tcagcatctg
     1561 tttattcgga agtccttccg gccttttaaa tgcttgcagt gtgggaaggc cttccgggaa
     1621 aaggacaaac tggaccagca cttacgcttc catgggcggg aggggaactg cccactgacc
     1681 tgtgatctct gtaacaaggg cttcatcagc agcacatcct tggagagcca catgaagctc
     1741 cactcagacc agaagactta ctcttgcatt ttttgcccag aatcctttga ccgccttgat
     1801 ttgttgaaag atcatgtggc cattcatatc aatgatggct acttcacctg cccaacttgt
     1861 aagaaacggt tcccagattt tatccaggtg aaaaaacacg tgcgcagctt ccactcagaa
     1921 aagatctacc agtgcacaga gtgtgacaag gccttctgtc gccccgataa actgcgactc
     1981 cacatgctcc ggcattcgga ccgcaaagac ttcctgtgtt ccacctgtgg gaagcaattt
     2041 aagcgaaaag acaaactacg ggaacacatg cagaggatgc ataatcctga gagggaggcc
     2101 aagaaagccg accgcatcag ccgctccaag acgttcaagc cccgcatcac gtccacagac
     2161 tacgacagct tcacgttcaa gtgccgcctg tgcatgatgg gcttccggcg gcgcggcatg
     2221 ctggtaaatc acttatcgaa gagacaccca gacatgaaga tagaagaggt gccagagtta
     2281 actctaccca tcataaaacc caatcgtgat tacttttgtc agtattgcga taaggtttat
     2341 aaaagtgcca gcaagcgcaa agcccacatt ctgaagaacc acccaggagc agagctccca
     2401 ccgagcattc ggaagctccg acccgctggt cctggagagc cagaccccat gctgagcaca
     2461 cacacccagc tgacgggcac catcgccacc cctcccgtct gctgtcccca ctgctccaag
     2521 cagtacagca gcaagaccaa gatggtccag cacattcgaa agaagcatcc agagttcgcc
     2581 cagctctcca acaccataca cacaccactg acgacagctg tgatcagtgc caccccagcg
     2641 gttttgacta cagacagcgc cactggagag actgtggtga cgacggacct gctcacccaa
     2701 gcaatgacag aactgtccca gaccttaacg acagactacc gaacgccaca aggggattac
     2761 cagagaattc agtacatccc tgtgtcgcag tcggcgtctg gcctccagca gcctcagcac
     2821 atacagctgc aagtggttca agtggcctcg gccacttccc ctcaccagtc acagcagtcc
     2881 actgtggatg ttggccagct ccatgatcct cagccctacc cccagcacgc catccaggtg
     2941 cagcacatcc aggtcagcga gcctaccgcc tcggccccgt cctccgccca ggtatctggg
     3001 cagccgttga gtccctcagc ccagcaggct cagcaggggc tcagcccctc ccacatccag
     3061 ggcagttctt ccacacaggg gcaggctctg cagcagcagc agcagcagca gcagaattcc
     3121 tctgtgcagc acacgtacct gcccagtgct tggaattcct tccgtggcta ttcatctgag
     3181 attcaaatga tgacgcttcc tccgggtcag tttgtgatta cagacagtgg tgtggcaact
     3241 ccagttacta ctggccaggt gaaggcggtt acttcgggtc attatgtgtt atcagaaagt
     3301 caatcagaat tggaagaaaa gcaaacttct gccctctctg gtggagtcca ggtcgagcca
     3361 cctgcacaca gtgactccct ggacccccag accaacagcc aacagcagac cacacagtac
     3421 atcatcacca ccaccaccaa cgggaacgga agcagcgaag tgcatatcac caaaccatag
     3481 taatgaacta gtccgtaact tgaaagtatt tcgatttctt ggctttatat atcttgtgga
     3541 aaggacgaca gcgctagatt ggcaccgctg ta
//
