LOCUS       sequence-282431-        3173 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3173)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 3173)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0688
FEATURES             Location/Qualifiers
     source          1..3173
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             3021..3038
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 accgccacca tgtctgctta ctctgtgcct tcaacttttg cccaggcctc attgccagtt
       61 cataaccagg tgctgccttc catcgagagt gtagatgggt ccgacccttt ggcaactctg
      121 cagacccctc taggcagact ggaggccaaa gaggaagagg atgaggatga ggacgaggac
      181 actgaggaag atgaggaaga agacggtgag gacacggatc tggatgactg ggagccagac
      241 ccgccccggc ccttcgaccc acacgacttg tggtgtgagg agtgcaataa cgcgcatgct
      301 tcagtgtgtc cgaagcacgg ccccttgcac ccgatcccca accggccggt gctcacccgg
      361 gccagggcga gcctccccct ggtgctctac atagacaggt ttctgggcgg ggtgttctcc
      421 aagcggcgca tccccaagcg cacccagttt ggccccgtgg aggggcctct cgtcaggggc
      481 tcggagctga aagactgtta cattcacctc aaggtttctc ttgataaagg ggacaggaaa
      541 gaaagggatt tacatgaaga cctatggttt gagttgtctg atgagacgct ttgtaactgg
      601 atgatgtttg tacggccagc ccagaatcac ctggagcaga acctggtggc ttaccagtat
      661 ggccaccatg tgtattatac aaccataaaa aatgtggagc ccaagcagga actgaaggtg
      721 tggtatgccg catcctatgc tgagttcgtg aaccagaaaa ttcatgacat ttctgaggaa
      781 gaaaggaaag ttcttcgaga gcaagagaag aattggccct gctatgaatg taaccgccga
      841 tttataagct cggagcagtt gcaacagcat ctcaattctc atgatgagaa actagatgtg
      901 tttagcagaa caagaggcag aggaagggga cgaggcaaga ggcgattcgg tccaggtcga
      961 cggccggggc gtcctccaaa atttatccgc ctggaaatca ccagcgaaaa tggggaaaag
     1021 agtgacgatg ggacacagga cttgctacat tttcccacaa aggagcaatt tgatgaggct
     1081 gaaccagcca ctctgaatgg gctggatcaa ccagaacaga ccactatccc aatccctcag
     1141 ctgccacagg aaacccagtc ttccctggaa catgaaccag aaactcacac cctgcacctg
     1201 cagccgcagc atgaagagag cgtggtgccc acccagagca cgctgacagc cgacgacatg
     1261 cgcagagcca agcgcatccg aaatgcagct cttcagcatc tgtttattcg gaagtccttc
     1321 cggcctttta aatgcttgca gtgtgggaag gccttccggg aaaaggacaa actggaccag
     1381 cacttacgct tccatgggcg ggaggggaac tgcccactga cctgtgatct ctgtaacaag
     1441 ggcttcatca gcagcacatc cttggagagc cacatgaagc tccactcaga ccagaagact
     1501 tactcttgca ttttttgccc agaatccttt gaccgccttg atttgttgaa agatcatgtg
     1561 gccattcata tcaatgatgg ctacttcacc tgcccaactt gtaagaaacg gttcccagat
     1621 tttatccagg tgaaaaaaca cgtgcgcagc ttccactcag aaaagatcta ccagtgcaca
     1681 gagtgtgaca aggccttctg tcgccccgat aaactgcgac tccacatgct ccggcattcg
     1741 gaccgcaaag acttcctgtg ttccacctgt gggaagcaat ttaagcgaaa agacaaacta
     1801 cgggaacaca tgcagaggat gcataatcct gagagggagg ccaagaaagc cgaccgcatc
     1861 agccgctcca agacgttcaa gccccgcatc acgtccacag actacgacag cttcacgttc
     1921 aagtgccgcc tgtgcatgat gggcttccgg cggcgcggca tgctggtaaa tcacttatcg
     1981 aagagacacc cagacatgaa gatagaagag gtgccagagt taactctacc catcataaaa
     2041 cccaatcgtg attacttttg tcagtattgc gataaggttt ataaaagtgc cagcaagcgc
     2101 aaagcccaca ttctgaagaa ccacccagga gcagagctcc caccgagcat tcggaagctc
     2161 cgacccgctg gtcctggaga gccagacccc atgctgagca cacacaccca gctgacgggc
     2221 accatcgcca cccctcccgt ctgctgtccc cactgctcca agcagtacag cagcaagacc
     2281 aagatggtcc agcacattcg aaagaagcat ccagagttcg cccagctctc caacaccata
     2341 cacacaccac tgacgacagc tgtgatcagt gccaccccag cggttttgac tacagacagc
     2401 gccactggag agactgtggt gacgacggac ctgctcaccc aagcaatgac agaactgtcc
     2461 cagaccttaa cgacagacta ccgaacgcca caaggggatt accagagaat tcagtacatc
     2521 cctgtgtcgc agtcggcgtc tggcctccag cagcctcagc acatacagct gcaagtggtt
     2581 caagtggcct cggtatctgg gcagccgttg agtccctcag cccagcaggc tcagcagggg
     2641 ctcagcccct cccacatcca gggcagttct tccacacagg ggcaggctct gcagcagcag
     2701 cagcagcagc agcagaattc ctctgtgcag cacacgtacc tgcccagtgc ttggaattcc
     2761 ttccgtggct attcatctga gattcaaatg atgacgcttc ctccgggtca gtttgtgatt
     2821 acagacagtg gtgtggcaac tccagttact actggccagg tgaaggcggt tacttcgggt
     2881 cattatgtgt tatcagaaag tcaatcagaa ttggaagaaa agcaaacttc tgccctctct
     2941 ggtggagtcc aggtcgagcc acctgcacac agtgactccc tggaccccca gaccaacagc
     3001 caacagcaga ccacacagta catcatcacc accaccacca acgggaacgg aagcagcgaa
     3061 gtgcatatca ccaaaccata gtaatgaact agtccgtaac ttgaaagtat ttcgatttct
     3121 tggctttata tatcttgtgg aaaggacgag cctccagttc tggtgtagac gaa
//
