LOCUS       sequence-282443-        4553 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4553)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 4553)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0730
FEATURES             Location/Qualifiers
     source          1..4553
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 accgccacca tgccatctga acagaaacag ttattttgtg atgaaaaaca aactacttta
       61 aaaaaagatt atgatgtgaa aaatgagata gttgataggt cggcacctaa accaaaaatt
      121 tcaggaagta ttcattatgc actaaaaaat gtgaaaattg atttgccaaa aataaatatt
      181 ccaaatgaag tcctattgaa acatgaagtt gacaaataca gaaaattatt tcagagtaaa
      241 cagcagactg caagaaaatc tatcagtata aagactgtaa gctgtgtaga ggagtgtaca
      301 ttgcttcata agtctgagag agctgaagaa gagggtgtaa aaatgtctgc aaaaatactc
      361 aatttcagct gtttaaaatg ccgagacaac actcgatata gcccaaatga tttgcagaaa
      421 cactttcaaa tgtggcacca tggcgaatta ccttcatatc cttgtgaaat gtgcaacttt
      481 tcagcaaatg actttcaggt atttaaacaa cacagacgaa cccatagaag cactttagta
      541 aaatgtgaca tttgtaacaa tgagagtgta tatactttac tgaacttgac aaagcatttc
      601 acatccacac attgtgttaa tggtaatttt caatgtgaaa agtgtaagtt ctccacccag
      661 gatgttggca catttgttca gcacattcat agacataatg aaattcatta taagtgtggt
      721 aaatgtcatc atgtatgttt taccaaagga gagcttcaga agcaccttca tattcattct
      781 ggtacttttc ccttcacttg tcaatattgt agctatggtg ccaccaggag agaacacctt
      841 gtaagacatg ttataacttt gcacaaagaa catttatatg caaaagaaaa actggaaaaa
      901 gacaaatatg aaaaaagaat ggcaaagact tctgcaggac ttaagctaat actgaaaaga
      961 tataaaatag gtgcatcaag gaagacgttc tggaaacgta agaaaattaa cagtggaagt
     1021 gacagaagta tagaaaagaa cactcaagtg cttaagaaaa tgaacaaaac acagactaaa
     1081 tctgaagacc agagccatgt tgttcaagag catttaagtg aagaaaagga tgaaagacta
     1141 cactgtgaga ataatgataa agcccctgaa tcagagtcag agaagccaac tcctctgtcc
     1201 actgggcaag gtaatagagc tgaagaggga ccaaacgcta gttcaggttt catgaagact
     1261 gctgtactag gacctacact gaaaaatgta atgatgaaaa ataataaact agcagtttcc
     1321 cctaactata atgctacgtt tatgggcttc aagatgatgg atggaaaaca gcatattgta
     1381 ttaaaattgg tgcctatcaa acaaaatgta tgttcaccag gctcacagtc aggtgctgca
     1441 aaggacggta ctgctaattt gcagccccag actttggaca ctaatggatt tttaacagga
     1501 gtaacaactg agttaaatga cacagtttat atgaaagcag ctactccatt ttcatgttca
     1561 tcttctatac tttcagggaa agcaagttca gaaaaagaaa tgactttgat atctcaaagg
     1621 aataatatgc ttcaaacaat ggattatgag aaaagtgtat cttctttgtc agcaacatca
     1681 gaattggtta cagcatcagt gaatttgacc acaaaatttg aaacaagaga taatgttgac
     1741 ttctggggaa atcatctcac tcagagtcac cccgaggtat taggtaccac cattaaaagt
     1801 ccagataaag tcaactgtgt tgccaaacca aatgcataca acagtggaga tatgcataat
     1861 tattgcatta attatggcaa ctgtgagtta cctgttgaat cctccaacca aggatcatta
     1921 ccttttcata attactcaaa agtgaataat tctaataaac gtcgtaggtt ttcaggaaca
     1981 gcagtgtatg aaaaccctca aagagaatct tcatccagca aaacagttgt ccaacaacca
     2041 attagtgaat catttttatc actagtgagg caggagagct caaaaccaga tagcctatta
     2101 gcatctatta gccttttaaa tgataaagat ggaactttaa aagcaaaatc tgaaattgaa
     2161 gaacagtatg ttttagaaaa aggacaaaac attgatggac aaaacctgta cagtaatgaa
     2221 aatcaaaatt tagagtgtgc gactgaaaaa tctaaatggg aagacttttc taatgtcgat
     2281 tcacctatga tgcctagaat cacatctgtt ttctctctcc agagccaaca ggcatcagaa
     2341 tttctgccac ctgaagtaaa ccaattgctt caggatgtat tgaaaataaa acctgatgta
     2401 aaacaagact ctagtaacac tccaaataaa ggcttgccac ttcattgtga ccagtcattt
     2461 caaaaacacg agagagaagg caaaattgtt gaatcttcga aagatttcaa agtgcaaggc
     2521 atcttcccag ttccacctgg cagtgtgggt attaatgtgc ctacaaatga tttgaatttg
     2581 aaatttggaa aagaaaaaca agtgtcatca ataccacaag atgtgagaga ttcagagaag
     2641 atgcctagaa tttcaggttt tggcacatta cttaagactc agtcagatgc gataataaca
     2701 cagcagcttg taaaagacaa actacgagcc accacacaaa atttaggttc tttttatatg
     2761 cagagtccac ttttaaattc agaacaaaaa aaaactataa ttgttcagac ttcaaaagga
     2821 ttcttaatac cattgaacat tactaacaag cctgggctac cagttattcc tggaaatgca
     2881 cttccattgg ttaattcaca aggtatccct gcttctcttt ttgtaaacaa gaaacctggg
     2941 atggttttaa cacttaataa tgggaaactt gaaggtgttt ccgctgtcaa aaccgagggt
     3001 gccccagctc gtggaactgt gactaaggag ccttgcaaaa cacctatttt gaaggtagaa
     3061 ccaaacaata attgtcttac acctggactt tgttccagca ttggcagttg tttgagcatg
     3121 aaaagtagct cagaaaatac tttgccatta aaaggccctt acattttgaa accaacgagt
     3181 tctgtgaaag ctgttcttat tcctaacatg ctatctgagc aacagagcac taagttgaat
     3241 atctccgatt cagtaaaaca gcagaatgag atttttccaa aaccacctct ttataccttc
     3301 ttgcctgatg gcaaacaagc tgttttttta aagtgtgtga tgccaaataa aactgagctg
     3361 cttaagccca aattagtcca aaatagtact tatcaaaata tacagccaaa gaaacctgaa
     3421 ggaacaccac aaagaatatt gctgaaaatt tttaaccctg ttttaaatgt gactgctgct
     3481 aataatctgt cagtaagcaa ctctgcatcc tcattgcaaa aagacaacgt accatctaat
     3541 cagattatag gaggagagca gaaagagcca gaatctagag atgccttacc cttcttacta
     3601 gatgacttaa tgccagcaaa tgaaattgtg ataacttcta ctgcaacatg cccagaatct
     3661 tctgaggaac caatatgtgt cagtgactgt tcagagtcca gggtattaag gtgtaaaaca
     3721 aattgtagaa ttgagaggaa cttcaataga aaaaagactt ccaaaaaaat tttttcaaaa
     3781 acaaaaactc atggaagtaa agactctgaa actgcctttg tatctagaaa cagaaactgt
     3841 aaacgaaagt gtagggatag ttaccaagaa cctccaagaa gaaaagcaac attgcataga
     3901 aagtgtaaag aaaaggcaaa acctgaagat gtccgtgaaa catttggatt tagcagacct
     3961 aggctttcaa aagattccat cagaactttg cggcttttcc cttttagttc taaacagctt
     4021 gtgaaatgtc ctaggagaaa ccaaccagtt gtagttttga atcatcctga cgcagatgca
     4081 ccagaagtag taagtgtaat gaaaactatt gctaaattta atggacatgt acttaaggtt
     4141 tcattgtcaa aaagaactat aaatgcttta ctgaaaccag tttgttataa ccctcctaaa
     4201 acaacttatg atgatttttc caagaggcac aaaacattta aacctgttag ttctgtgaaa
     4261 gaaagatttg tgctaaaatt aacactcaaa aagacaagca aaaacaatta ccagattgtg
     4321 aagactacct ctgaaaatat tttgaaggct aaatttaact gttggttttg tggtagagta
     4381 tttgacaatc aggatacttg ggctggtcat gggcagagac atttaatgga agctactcgg
     4441 gattggaaca tgttagaata gtaatgaact agtccgtaac ttgaaagtat ttcgatttct
     4501 tggctttata tatcttgtgg aaaggacgag ttagtttggc ggcgcattct cct
//
