LOCUS       sequence-282452-        7322 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 7322)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 7322)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0753
FEATURES             Location/Qualifiers
     source          1..7322
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggatcctga acaaagtgtc aagggcacca agaaggctga gggaagtccc
       61 cggaagcggc tgaccaaagg agaggccatt cagaccagtg tttcttccag cgtcccatac
      121 ccaggcagcg gcacagctgc cacccaagag agccccgccc aagagctctt agccccgcag
      181 cccttcccgg gcccctcatc agttcttagg gaaggctctc aggagaaaac gggccagcag
      241 cagaagcccc ccaaaaggcc ccccatcgaa gcatccgtcc acatctcaca gcttccgcag
      301 caccctctga caccagcatt catgtcgcct ggcaaacctg agcatctcct ggaggggtcc
      361 acatggcaac tggttgaccc catgagacct ggaccctctg gctccttcgt ggcccctggg
      421 ctccatcctc agagccagct ccttccttcc cacgcttcca tcattccccc cgaggacctt
      481 cctggagtcc ccaaagtctt cgtgcctcgt ccttcccagg tctccttgaa gcccacagaa
      541 gaggcacaca agaaggagag gaagccccag aagccaggca agtacatctg ccagtactgc
      601 agccggccct gtgccaagcc cagcgtgctc cagaagcaca ttcgctcaca cacaggtgag
      661 aggccctacc cctgcggccc ctgtggcttc tccttcaaga ccaagagtaa tctctacaag
      721 cacaggaagt cccatgccca ccgcatcaaa gcaggcctgg cctcaggcat gggtggcgag
      781 atgtacccac atgggctgga gatggagcgg atccctgggg aagagtttga ggagcccact
      841 gagggagaaa gcacagattc tgaagaggag actagtgcca cctctggtca ccctgcagag
      901 ctctccccaa gacccaagca gccccttctc tccagcgggc tatacagctc tgggagccac
      961 agttccagcc acgaacgctg ttccctgtcc cagtccagca cagcccagtc actcgaagac
     1021 ccccctccat ttgtggaacc ctcatctgag caccccctga gccataaacc tgaagacacc
     1081 cacacgatta agcagaagct ggccctccgc ttaagcgaga ggaagaaggt gatcgatgag
     1141 caggcgtttc tgagcccagg cagcaaaggg agtactgagt ctgggtattt ctctcgctcc
     1201 gagagtgcag agcagcaggt cagcccccca aacaccaacg ccaagtccta cgctgagatc
     1261 atctttggca agtgtgggcg aataggacag cggaccgcca tgctgacagc cacctccacc
     1321 cagcccctcc tgcccctgtc caccgaagac aagcccagcc tggtgccttt gtctgtaccc
     1381 cggacgcagg tgatcgagca catcacgaag ctcatcacca tcaacgaggc cgtggtggac
     1441 accagcgaga tcgacagcgt gaagccaagg cggagctcac tgtccaggcg cagcagcatg
     1501 gagtccccaa aatccagcct ctaccgggag cccctgtcat cccacagtga gaaaaccaag
     1561 cctgaacaat cactgctgag cctccagcac ccgcccagta ccgccccccc tgtgcctctc
     1621 ctgagaagcc actcaatgcc ttctgccgcc tgcactatca gcacccccca ccaccccttc
     1681 cgaggtagct actccttcga tgaccatatc accgactccg aagccctgag ccacagcagt
     1741 cacgtgttta cctcccaccc ccggatgctg aagcgccagc cggcaatcga attacctttg
     1801 ggaggggaat acagttctga ggagcctggc ccaagcagca aagacacagc ctccaagccc
     1861 tcggacgaag tggaacccaa ggaaagcgag cttaccaaaa agaccaagaa gggtttgaaa
     1921 acaaaagggg tgatctacga atgtaacata tgtggtgctc ggtacaagaa aagggataac
     1981 tacgaagccc acaaaaaata ctactgctca gagcttcaga tcgcaaagcc catctctgca
     2041 ggcacccaca catctccaga agctgaaaag agtcagattg agcatgagcc gtggtcccaa
     2101 atgatgcatt acaaactggg aaccaccctg gaactcactc cactgaggaa gaggaggaaa
     2161 gagaagagcc ttggggacga ggaagagcca cctgcctttg agtccacaaa aagtcagttt
     2221 ggcagccccg ggccatctga tgctgctcgg aaccttcccc tggagtccac caagtcacca
     2281 gcagaaccaa gtaaatcagt gccctccttg gagggaccca cgggcttcca gccaaggact
     2341 cccaagccag ggtccggttc agaatcaggg aaggagagga gaacaacgtc caaagaaatt
     2401 tctgtcatcc agcacaccag ctcctttgag aaatctgatt ctctcgagca gccgagtggc
     2461 ttggaagggg aagacaaacc tctggcccag ttcccatcac ccccacctgc cccacacgga
     2521 cgctctgctc actccctgca gcctaagttg gtccgccagc ccaacattca ggttcctgag
     2581 atcctagtaa ctgaggagcc tgaccggccg gacacagagc cagagccgcc ccctaaggaa
     2641 cctgagaaga ctgaggagtt ccaatggccc cagcgcagcc agacacttgc ccagctccca
     2701 gctgagaagc tgccacccaa aaagaagagg ttgcgcctgg cagagatggc ccaatcatca
     2761 ggggagtcca gcttcgagtc ctctgtgcct ctgtctcgca gcccgagcca ggaaagcaat
     2821 gtctctttga gtgggtccag ccgctcagcc tcgtttgaga gggatgacca tgggaaagcc
     2881 gaggccccca gtccctcatc tgacatgcgc cccaaacccc tgggcaccca catgttgact
     2941 gtccccagcc accacccaca tgcccgagag atgcggaggt cagcctcaga gcagagcccc
     3001 aacgtttccc attctgccca catgaccgag acacgcagca aatcctttga ctatggcagc
     3061 ttgtccttga caggcccttc tgctccagcc ccagtggctc caccagcgcg ggtggccccg
     3121 ccagagagaa gaaaatgctt cttggtgaga caggcctctc tgagcaggcc tccagaatct
     3181 gagttggagg ttgcccccaa gggaagacag gagagcgaag aaccacagcc ctcatccagt
     3241 aaaccctctg ccaaaagctc attgtcccag atttcctctg cggccacctc acatggtgga
     3301 cccccgggag gcaagggccc agggcaggac aggcccccat tggggcccac tgtgccctac
     3361 acagaagcac tgcaagtgtt ccaccacccc gttgcccaga cacccctgca tgagaagcca
     3421 tacctgcccc caccagtctc ccttttctcc ttccagcatc tcgtgcagca tgagccagga
     3481 cagtctccag aattcttctc cacccaggcc atgtccagcc tcctgtcctc accatactcc
     3541 atgcccccac ttcctccctc cttatttcaa gccccaccgc ttcctctcca gcctactgtt
     3601 ctgcacccag gccaactcca tctcccccag ctcatgcctc acccagccaa catccccttc
     3661 aggcagcccc cttccttcct ccccatgcca tacccgacct cctcagcact gtcttctggg
     3721 tttttcctgc ctctgcaatc ccagtttgca cttcagctcc ctggtgatgt ggaaagccat
     3781 ctgccccaga tcaaaaccag cctggcccca ctggcaacag gaagtgctgg cctctccccc
     3841 agcacagagt acagcagtga catccggcta ccccctgtgg ctcccccagc cagctcctca
     3901 gcacctacat cagctcctcc actggccctg cctgcctgtc cagacaccat ggtgtccctg
     3961 gttgtgcctg tccgtgttca gaccaatatg ccgtcctatg ggagcgcaat gtacaccacc
     4021 ctttcccaga tcttggtcac ccagtcccaa ggcagctcag caactgtggc acttcccaag
     4081 tttgaggaac ccccatcaaa ggggacgact gtatgtggtg cagatgtgca tgaggttggg
     4141 cccggccctt ctgggttaag tgaagagcaa agcagagctt tcccaactcc atacctgaga
     4201 gtgcctgtga cattacctga aagaaaaggc acttccctgt catcagagag tatcttgagc
     4261 ctggagggga gttcatcaac agcaggggga agcaaacgtg tcctttcacc agctggcagc
     4321 cttgaactta ccatggaaac ccagcagcaa aaaagagtga aggaggagga ggcttccaag
     4381 gcagatgaaa aacttgagct ggtaaaacca tgcagtgtgg tccttaccag caccgaggat
     4441 gggaagaggc cagagaaatc ccacttaggc aaccagggcc aaggcaggag ggagctagaa
     4501 atgctgtcca gcctgtcctc agatccatct gacacaaagg aaattcctcc cctccctcac
     4561 cctgcattgt cccatgggac agccccaggc tcagaagctt tgaaggaata tccccagcca
     4621 tctggcaaac ctcaccgaag agggttgacc ccactgagcg tgaagaaaga agattccaag
     4681 gaacaacctg atctcccctc cttggcacct ccgagctctc tgcctctgtc agaaacgtcc
     4741 tccagaccag ccaagtcaca agaaggtacg gactcaaaga aggtactgca gttccccagc
     4801 ctccacacaa ccactaatgt cagttggtgc tatttaaact acattaagcc aaatcacatc
     4861 cagcatgcag ataggaggtc ctctgtttac gctggttggt gcataagttt gtacaacccc
     4921 aaccttccgg gggtttccac taaagctgct ttgtccctcc tgaggtctaa gcagaaagtg
     4981 agcaaagaga catacaccat ggccacagct ccgcatcctg aggcaggaag gcttgtgcca
     5041 tccagctccc gcaagccccg catgacagag gttcacctcc cttcactggt ttccccggaa
     5101 ggccagaaag atctagctag agtggagaag gaagaagaga ggagagggga gccggaggag
     5161 gatgctcctg cctcccagag aggggagccg gcgaggatca aaatcttcga aggagggtac
     5221 aaatcaaacg aagagtatgt atatgtgcga ggccgcggcc gagggaaata tgtttgtgag
     5281 gagtgtggaa ttcgctgcaa gaagcccagc atgctgaaga aacacatccg cacccacact
     5341 gacgtccggc cctatgtgtg caagcactgt cactttgctt ttaaaaccaa agggaatctg
     5401 actaagcaca tgaagtcgaa ggcccacagc aaaaagtgcc aagagacagg ggtgctggag
     5461 gagctggaag ccgaagaagg aaccagtgac gacctgttcc aggactcgga aggacgagag
     5521 ggttcagagg ctgtggagga gcaccagttt tcggacctgg aggactcgga ctcagactca
     5581 gacctggacg aagacgagga tgaggatgag gaggagagcc aggatgagct gtccagacca
     5641 tcctcagagg cgcccccgcc tggcccacca catgcactgc gggcagactc ctcacccatc
     5701 ctgggccctc agcccccaga tgcccccgcc tctggcacgg aggctacacg aggcagctcg
     5761 gtctcggaag ctgagcgcct gacagccagc agctgctcca tgtccagcca gagcatgccg
     5821 ggcctcccct ggctgggacc ggcccctctg ggctctgtgg agaaagacac aggctcagcc
     5881 ttgagctaca agcctgtgtc cccaagaaga ccgtggtccc caagcaaaga agcaggcagc
     5941 cgtccaccac tagcccgcaa acactcgcta accaaaaacg actcatctcc ccagcgatgc
     6001 tccccggccc gagaaccaca ggcctcagcc ccaagcccac ctggcctgca cgtggaccca
     6061 ggaaggggca tgggcgctct cccttgtggg tctccaagac ttcagctgtc tcctctcacc
     6121 ctctgccccc tgggaagaga actggcccct cgagcacatg tgctctccaa actcgagggt
     6181 accaccgacc caggcctccc cagatactcg cccaccagga gatggtctcc aggtcaggcc
     6241 gagtcaccac cacggtcagc gccgccaggg aagtgggcct tggctgggcc gggcagcccc
     6301 tcagcggggg agcatggccc aggcttgggg ctggacccac gggttctctt cccgcccgcg
     6361 cctctacctc acaagctcct cagcagaagc ccagagacct gcgcctcccc gtggcagaag
     6421 gccgagtccc gaagtccctc ctgctcaccc ggccctgctc atcctctctc ctcccgaccc
     6481 ttctccgccc tccatgactt ccacggccac atcctggccc ggacagagga gaacatcttc
     6541 agccacctgc ctctgcactc ccagcacttg acccgtgccc catgtccctt gattcccatc
     6601 ggtgggatcc agatggtgca ggcccggcca ggagcccacc ccaccctgct gccagggccc
     6661 accgcagcct gggtcagtgg cttctccggg ggtggcagcg acctgacagg ggcccgggag
     6721 gcccaggagc gaggccgctg gagtcccact gagagctcgt cagcctccgt gtcgcctgtg
     6781 gctaaggtct ccaaattcac actctcctca gagctggagg gcggggacta ccccaaggag
     6841 agggagagga ccggcggagg cccgggcagg cctcctgact ggacacccca tgggaccggg
     6901 gcacctgcag agcccacacc cacgcacagc ccctgcaccc cacccgacac cttgccccgg
     6961 ccgccccagg gacgccgggc agcgcagtcc tggagccccc gcttggagtc cccgcgtgca
     7021 ccgaccaacc ccgagccttc tgccaccccg ccgctggacc gcagcagctc tgtgggctgc
     7081 ctggcagagg cctctgcccg cttcccagcc cggacgagga acctctccgg ggaacccagg
     7141 accaggcagg actcccccaa gccctcagga agtggggagc ccagggcaca tccacatcag
     7201 cctgaggaca gggttccccc caacgcttag taatgaacta gtccgtaact tgaaagtatt
     7261 tcgatttctt ggctttatat atcttgtgga aaggacgacc aggaagaggg ttaacccaca
     7321 gc
//
