LOCUS       sequence-282461-        1328 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1328)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1328)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0813
FEATURES             Location/Qualifiers
     source          1..1328
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggagttggc gactcgctac cagatcccta aagaagtggc tgacatcttt
       61 aacgccccca gtgatgatga agagtttgtt ggcttccgag atgatgttcc catggaaacc
      121 ctctcgtcag aggagagctg cgatagtttt gactcactag agtcagggaa acaggtcgtg
      181 gagtcagatt tgagtgatga tggcaaagca tctttggtga gcgaggaaga ggaagatgaa
      241 gaagaagata aggctacccc tagaagaagc aggtctagaa gaagtagtat tggtcttcga
      301 gtagcctttc agttccccac caagaagctg gccaacaaac cagataaaaa cagttcttcc
      361 gagcagttgt tttctagcgc acgcttacag aatgagaaaa aaacaattct tgaaagaaag
      421 aaagactgta gacaggtgat acaaagggaa gattctacct ctgagtctga ggatgactct
      481 cgggatgaga gccaggagag ttcagatgct ttgctgaaaa ggaccatgaa catcaaggag
      541 aacaaagcca tgcttgccca gttattggcg gaattgaact cgatgccaga tttcttccca
      601 gtacgaaccc caacctcagc ttctaggaag aagacagtga ggcgggcctt ctcggaggga
      661 cagatcacgc ggcgtatgaa cccaacccgg agtgcgcggc ctcctgagaa gtttgctcta
      721 gagaacttca ctgtctcagc cgctaaattt gcggaagagt tttacagctt ccgaagaagg
      781 aagacaattg gggggaaatg ccgggagtac agacgacgtc accgtatatc ttcttttcgg
      841 ccagtggagg atatcaccga agaggactta gaaaatgttg ccataactgt tcgagataaa
      901 atctatgata aagttctggg taacacgtgc catcagtgtc gacaaaagac catcgacacc
      961 aagacagtgt gtcggaacca gggttgctgt ggtgtgcgag gacagttctg tggaccatgc
     1021 ctgcggaacc gctatgggga ggatgtcaga tcggcattgc tggacccgga ttgggtgtgt
     1081 cccccctgtc gtgggatctg caattgcagc tactgtcgga agcgtgacgg ccgctgtgcc
     1141 acaggaatcc tcattcatct ggccaagttt tatggttatg acaatgttaa ggaatatctg
     1201 gagagcttac aaaaggagct ggtagaagac aattagtaat gaactagtcc gtaacttgaa
     1261 agtatttcga tttcttggct ttatatatct tgtggaaagg acgaacgaac ttctggtctg
     1321 tataagct
//
