LOCUS       sequence-282464-        1013 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1013)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1013)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0847
FEATURES             Location/Qualifiers
     source          1..1013
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             30..50
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=7xHis
                     /translation="HHHHHHH"
ORIGIN
        1 accgccacca tgagtgaggc gggcgaggcc accaccacca ccaccaccac cctcccgcag
       61 gctccgacgg aggcggccgc cgcggctccc caggaccccg cgcccaagag cccggtgggc
      121 agcggtgcgc cccaggccgc ggccccggcg cccgccgccc acgtcgcagg aaaccccggt
      181 ggggacgcgg cccccgcagc cacgggcacc gcggccgccg cctctttagc caccgccgcc
      241 ggcagcgaag acgcggagaa aaaagttctc gccaccaaag tccttggcac tgtcaaatgg
      301 ttcaacgtca gaaatggata tggatttata aatcgaaatg acaccaaaga agatgtattt
      361 gtacatcaga ctgccatcaa gaagaataac ccacggaaat atctgcgcag tgtaggagat
      421 ggagaaactg tagagtttga tgtggttgaa ggagagaagg gtgcagaagc tgccaatgtg
      481 actggcccgg atggagttcc tgtggaaggg agtcgttacg ctgcagatcg gcgccgttac
      541 agacgtggct actatggaag gcgccgtggc cctccccgga atgctggtga gattggagag
      601 atgaaggatg gagtcccaga gggagcacaa cttcagggac cggttcatcg aaatccaact
      661 taccgcccaa ggtaccgtag caggggacct cctcgcccac gacctgcccc agcagttgga
      721 gaggctgaag ataaagaaaa tcagcaagcc accagtggtc caaaccagcc gtctgttcgc
      781 cgtggatacc ggcgtcccta caattaccgg cgtcgcccgc gtcctcctaa cgctccttca
      841 caagatggca aagaggccaa ggcaggtgaa gcaccaactg agaaccctgc tccacccacc
      901 cagcagagca gtgctgagta gtaatgaact agtccgtaac ttgaaagtat ttcgatttct
      961 tggctttata tatcttgtgg aaaggacgag tgcccattag caacacatgg cct
//
