LOCUS       sequence-282467-        1220 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1220)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1220)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0848
FEATURES             Location/Qualifiers
     source          1..1220
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             30..50
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=7xHis
                     /translation="HHHHHHH"
ORIGIN
        1 accgccacca tgagtgaggc gggcgaggcc accaccacca ccaccaccac cctcccgcag
       61 gctccgacgg aggcggccgc cgcggctccc caggaccccg cgcccaagag cccggtgggc
      121 agcggtgcgc cccaggccgc ggccccggcg cccgccgccc acgtcgcagg aaaccccggt
      181 ggggacgcgg cccccgcagc cacgggcacc gcggccgccg cctctttagc caccgccgcc
      241 ggcagcgaag acgcggagaa aaaagttctc gccaccaaag tccttggcac tgtcaaatgg
      301 ttcaacgtca gaaatggata tggatttata aatcgaaatg acaccaaaga agatgtattt
      361 gtacatcaga ctgccatcaa gaagaataac ccacggaaat atctgcgcag tgtaggagat
      421 ggagaaactg tagagtttga tgtggttgaa ggagagaagg gtgcagaagc tgccaatgtg
      481 actggcccgg atggagttcc tgtggaaggg agtcgttacg ctgcagatcg gcgccgttac
      541 agacgtggct actatggaag gcgccgtggc cctccccgga attacgctgg ggaggaggag
      601 gaggaaggga gcggcagcag tgaaggattt gacccccctg ccactgatag gcagttctct
      661 ggggcccgga atcagctgcg ccgcccccag tatcgccctc agtaccggca gcggcggttc
      721 ccgccttacc acgtgggaca gacctttgac cgtcgctcac gggtcttacc ccatcccaac
      781 agaatacagg ctggtgagat tggagagatg aaggatggag tcccagaggg agcacaactt
      841 cagggaccgg ttcatcgaaa tccaacttac cgcccaaggt accgtagcag gggacctcct
      901 cgcccacgac ctgccccagc agttggagag gctgaagata aagaaaatca gcaagccacc
      961 agtggtccaa accagccgtc tgttcgccgt ggataccggc gtccctacaa ttaccggcgt
     1021 cgcccgcgtc ctcctaacgc tccttcacaa gatggcaaag aggccaaggc aggtgaagca
     1081 ccaactgaga accctgctcc acccacccag cagagcagtg ctgagtagta atgaactagt
     1141 ccgtaacttg aaagtatttc gatttcttgg ctttatatat cttgtggaaa ggacgacaag
     1201 actagtacgt ccgtattgtt
//
