LOCUS       sequence-282475-       12826 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12826)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 12826)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0886
FEATURES             Location/Qualifiers
     source          1..12826
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1770..1980
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1792..1988
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1839..1974
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2020..2622
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2020..2039)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2516..2536
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2665..2782
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2920..4098
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3151..4089
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4046..4066
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      4109..4118
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    8683..9271
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8736..8756)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9154..9165)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             9343..9576
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    9654..9775
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9691..9710)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9745..9764
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      9815..9950
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(9970..9989)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9971..9989)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9999..10016)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9999..10015)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10008..10030)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      10157..10612
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10244..10263)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10454..10475
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        10638..10742
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10743..10811,10812..11603)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10961..10980)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11774..12362
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12263..12282
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     12516..12533
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    12650..12671
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(12686..12691,12692..12709,12710..12716)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12724..12740
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     12729..12751
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     12748..12764
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     12748..12764
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     12783..12803
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        12785..12803
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat tagtcagcaa
     1741 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcgtctca
     1801 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     1861 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     1921 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     1981 tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta caagcccacg
     2041 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     2101 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     2161 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     2221 gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     2281 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     2341 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     2401 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     2461 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     2521 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     2581 ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct agatcttgag
     2641 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     2701 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     2761 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg
     2821 ccggctcgag gcctgcaggt gcaaagatgg ataaagtttt aaacagagag gaatctttgc
     2881 agctaatgga ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg
     2941 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
     3001 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     3061 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     3121 tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct
     3181 ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac
     3241 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct
     3301 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc
     3361 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt
     3421 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg
     3481 ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt
     3541 gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga
     3601 cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc
     3661 gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg
     3721 ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg
     3781 gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt
     3841 gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt
     3901 acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg
     3961 gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt
     4021 tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt
     4081 ccatttcagg tgtcgtgagg ctagcaccgc caccatggcc gccaatgtgg gatcgatgtt
     4141 tcaatattgg aagcgatttg atctacggcg actccagaag gagcttaatt ccgtcgcttc
     4201 tgagctgtct gcacggcagg aggagagtga acattctcat aaacatttaa ttgaactccg
     4261 ccgggaattt aagaaaaatg tacctgagga aatcagagag atggtggctc ctgtattaaa
     4321 aagcttccaa gccgaggtgg tggcccttag taagagaagt caggaggcgg aggctgcttt
     4381 tctgagtgtt tacaagcaat taattgaagc accagacccc gtgcctgtgt ttgaggcggc
     4441 acgcagccta gacgacagac tgcagccccc cagctttgac cccagtgggc agccccggcg
     4501 agacctccac acttcgtgga agaggaaccc cgagctcctc agccccaaag agcagagaga
     4561 ggggacgtcg cctgccgggc ccacgctgac cgagggaagc cgcctcccag gcattcccgg
     4621 gaaagccctc ctgacagaaa ccttgctgca gagaaatgag gcggaaaaac aaaagggcct
     4681 tcaagaagta cagatcactt tggcggccag actgggggag gcagaggaga aaatcaaagt
     4741 cctacattca gcgctaaagg ctacgcaggc agagctgcta gagctgcggc ggaagtacga
     4801 cgaggaggca gcatccaagg cagatgaagt cggcctgatc atgaccaacc tggagaaagc
     4861 taatcagcga gctgaggctg cccagcggga ggtggaaagt ctccgggaac agctggcctc
     4921 tgtcaacagc tccatccgcc tggcttgctg ctctccccag gggcccagtg gggataaggt
     4981 gaacttcact ctgtgctcgg gccctcggct ggaggccgcg ctggcctcca aggacaggga
     5041 gatcctgcgg ctgctgaagg acgtgcagca cctccagagc tcactgcagg agctggagga
     5101 ggcatccgcc aaccagatcg ccgacctgga gcggcagctc acggccaagt ccgaggccat
     5161 agaaaagctg gaagagaagc tccaggccca gtctgactat gaggaaatta aaacggagct
     5221 gagcatcctg aaagccatga agctggcctc cagcacctgc agcctccccc agggcatggc
     5281 caagcctgaa gactcactgc ttattgcaaa ggaggccttc ttccccacgc agaaattcct
     5341 tctggagaag cccagcctcc tggccagccc tgaggaagac ccatcagagg acgattccat
     5401 caaggattca ctgggcacgg agcagtccta cccctcccct cagcagctcc cacctccacc
     5461 agggccagaa gaccccctgt ctcccagccc cgggcagccc ctgctgggcc ccagcttggg
     5521 gcctgacggc actcggactt tctcgctgtc ccccttcccc agcctggcat caggggagag
     5581 actgatgatg cccccagccg ccttcaaggg agaggcgggc ggcctgctgg tgttcccccc
     5641 agccttctat ggcgccaagc cccccacagc ccctgccacc ccggcccctg gccctgagcc
     5701 actgggcggt cctgagcccg cggatggtgg tgggggcgga gcggcggggc ccggggcaga
     5761 ggaggagcag ctggacacgg cagagatcgc cttccaggtg aaggagcagc tgctgaaaca
     5821 caacatcggg cagcgggtgt ttgggcatta cgtgctgggg ctgtcgcagg gctcggtcag
     5881 cgagatccta gcccggccca agccctggcg caagctcacg gtgaagggca aggagccctt
     5941 catcaagatg aagcagttcc tgtcggatga gcagaatgta ctggcgctca ggaccatcca
     6001 agtgcggcag cgaggcagca tcaccccgag aatccgcacg cctgagacag gctcagacga
     6061 cgccatcaag agcattctag agcaggccaa gaaggagatc gagtcgcaga agggcggcga
     6121 gcccaagacc tcggtggccc cgctgagcat cgccaacggc acgacccccg ccagcacctc
     6181 ggaggacgcc atcaagagca tcctggagca ggcacgccgt gagatgcagg cgcaacagca
     6241 ggcgctgctg gagatggagg tggcgcccag gggccgctcg gtgcccccct cgcccccgga
     6301 gcggccatca ctggccaccg cgagccagaa cggggccccg gccttggtga agcaggagga
     6361 gggcagcggg ggccccgcgc aggcgccgct cccggtcctg tcccccgccg ccttcgtgca
     6421 gagcatcatc cgcaaggtca agtccgagat cggcgacgcc ggctacttcg accaccactg
     6481 ggcctccgac cgcggcctgc tcagccgccc ctacgcctcc gtgtcgccct cgctgtcctc
     6541 ctcctcctcc tctggctact ctggccagcc caacggccgc gcctggcccc gcggggacga
     6601 ggcccctgtg ccccccgagg acgaggcggc ggcaggggcg gaggacgaac cccccaggac
     6661 gggcgagctc aaggctgagg gcgcgacggc cgaggcgggc gcgcggctgc cctactaccc
     6721 ggcctacgtg ccgcgcaccc tgaagcccac cgtgccgccg ctgacccccg agcagtacga
     6781 gctgtacatg taccgtgagg tagacacgct ggagctcacc cgccaggtca aggagaagct
     6841 ggccaagaac ggcatctgcc agaggatctt cggggagaag gtgctgggcc tgtcacaggg
     6901 cagcgtgagc gacatgctgt cccggccgaa gccatggagc aagctgacgc agaaggggcg
     6961 ggagcccttc atccgcatgc agctgtggct ctctgaccag ctcggccagg cagtgggcca
     7021 gcagcctggt gcctcccagg ccagtcccac agaaccaagg tcctcaccat ccccaccccc
     7081 cagccccaca gagcctgaga agagctccca ggagccgttg agcctgtccc tggagagcag
     7141 caaggagaac cagcagccag agggccgctc cagctcctcg ttgagcggga agatgtactc
     7201 aggcagccag gccccagggg gcatccagga gatcgtggcc atgtcccccg agctggacac
     7261 gtactccatc accaagaggg tgaaggaggt cctcacagac aacaatctag ggcagcggct
     7321 gtttggggaa agcatcctgg gtctgacaca gggctccgtg tctgacctgc tgtcccggcc
     7381 caaaccctgg cacaagctga gcctgaaggg gcgggagcct tttgtccgca tgcagctgtg
     7441 gctcaatgac ccccataacg tggagaagct gagggatatg aagaagctgg agaagaaagc
     7501 ctacctgaaa cgtcgctatg gcctcatcag caccggctca gacagtgagt ccccggccac
     7561 ccgctcagag tgccccagcc cctgcctgca gccccaggac ctgagcctcc tgcagatcaa
     7621 gaagccccgg gtggtgctgg cacccgagga gaaggaggca ctgcggaagg cctatcagct
     7681 ggaaccctac ccctcgcagc agaccatcga gctcctctcc ttccagctca acctcaagac
     7741 caacaccgtc atcaactggt tccacaacta caggtcccgg atgcgccggg agatgttggt
     7801 ggaggggacc caggatgagc cagaccttga tccaagcggg ggtcctggaa tcctaccgcc
     7861 aggccactcc cacccagacc ccaccccgca gagccctgac tctgagactg aggaccagaa
     7921 gccaaccgtg aaggaactgg agcttcagga gggccctgag gagaacagca cacccctgac
     7981 cacccaggac aaggcccaag tgaggatcaa gcaggaacag atggaggagg atgctgagga
     8041 agaggcaggc agccagcccc aggactcagg ggagctggac aaaggccaag gtccccccaa
     8101 agaggagcat cccgaccctc cgggtaatga tggactccca aaagtggctc ccgggcccct
     8161 ccttccaggt ggatccaccc cagactgtcc ctcacttcat ccccaacagg agagtgaggc
     8221 cggggagcga cttcacccgg accctttaag ttttaagtca gcctcagagt cctcacgctg
     8281 cagcctggag gtgtcactga actcgccctc ggccgcctcc tcaccaggcc tcatgatgtc
     8341 tgtgtcacct gtcccctcct cctcagctcc catctcccca tccccacctg gcgccccccc
     8401 tgccaaagtg ccgagtgcca gccccactgc tgacatggct ggagccttgc accccagtgc
     8461 caaggtgaac cccaacttgc agcggcggca tgagaagatg gccaatctga acaacatcat
     8521 ttaccgagta gagcgggctg ccaatcggga ggaggccctg gagtgggagt tctagtaatg
     8581 aactagtccg taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga
     8641 cgactaggac tagagaggtc gcggaaaacg cgttaagtcg acaatcaacc tctggattac
     8701 aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga
     8761 tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc
     8821 tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa
     8881 cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc
     8941 acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc
     9001 atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc
     9061 gtggtgttgt cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg
     9121 attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct
     9181 tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg
     9241 agtcggatct ccctttgggc cgcctccccg cgtcgacttt aagaccaatg acttacaagg
     9301 cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact
     9361 cccaacgaag acaagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct
     9421 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
     9481 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
     9541 tcagaccctt ttagtcagtg tggaaaatct ctagcagtac gtatagtagt tcatgtcatc
     9601 ttattattca gtatttataa cttgcaaaga aatgaatatc agagagtgag aggaacttgt
     9661 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
     9721 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
     9781 tctggctcta gctatcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
     9841 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
     9901 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc tagggacgta
     9961 cccaattcgc cctatagtga gtcgtattac gcgcgctcac tggccgtcgt tttacaacgt
    10021 cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc
    10081 gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc
    10141 ctgaatggcg aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt
    10201 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc
    10261 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct
    10321 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat
    10381 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc
    10441 acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc
    10501 tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg
    10561 atttaacaaa aatttaacgc gaattttaac aaaatattaa cgcttacaat ttaggtggca
    10621 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
    10681 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
    10741 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
    10801 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
    10861 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
    10921 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
    10981 cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
    11041 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
    11101 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
    11161 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
    11221 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
    11281 tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag
    11341 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
    11401 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt
    11461 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
    11521 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
    11581 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg
    11641 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
    11701 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
    11761 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
    11821 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
    11881 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
    11941 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
    12001 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
    12061 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
    12121 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
    12181 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
    12241 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
    12301 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
    12361 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
    12421 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
    12481 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
    12541 aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct
    12601 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt
    12661 agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg
    12721 gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccaagc
    12781 gcgcaattaa ccctcactaa agggaacaaa agctggagct gcaagc
//
