LOCUS       sequence-282476-        4562 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4562)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 4562)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0886
FEATURES             Location/Qualifiers
     source          1..4562
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggccgccaa tgtgggatcg atgtttcaat attggaagcg atttgatcta
       61 cggcgactcc agaaggagct taattccgtc gcttctgagc tgtctgcacg gcaggaggag
      121 agtgaacatt ctcataaaca tttaattgaa ctccgccggg aatttaagaa aaatgtacct
      181 gaggaaatca gagagatggt ggctcctgta ttaaaaagct tccaagccga ggtggtggcc
      241 cttagtaaga gaagtcagga ggcggaggct gcttttctga gtgtttacaa gcaattaatt
      301 gaagcaccag accccgtgcc tgtgtttgag gcggcacgca gcctagacga cagactgcag
      361 ccccccagct ttgaccccag tgggcagccc cggcgagacc tccacacttc gtggaagagg
      421 aaccccgagc tcctcagccc caaagagcag agagagggga cgtcgcctgc cgggcccacg
      481 ctgaccgagg gaagccgcct cccaggcatt cccgggaaag ccctcctgac agaaaccttg
      541 ctgcagagaa atgaggcgga aaaacaaaag ggccttcaag aagtacagat cactttggcg
      601 gccagactgg gggaggcaga ggagaaaatc aaagtcctac attcagcgct aaaggctacg
      661 caggcagagc tgctagagct gcggcggaag tacgacgagg aggcagcatc caaggcagat
      721 gaagtcggcc tgatcatgac caacctggag aaagctaatc agcgagctga ggctgcccag
      781 cgggaggtgg aaagtctccg ggaacagctg gcctctgtca acagctccat ccgcctggct
      841 tgctgctctc cccaggggcc cagtggggat aaggtgaact tcactctgtg ctcgggccct
      901 cggctggagg ccgcgctggc ctccaaggac agggagatcc tgcggctgct gaaggacgtg
      961 cagcacctcc agagctcact gcaggagctg gaggaggcat ccgccaacca gatcgccgac
     1021 ctggagcggc agctcacggc caagtccgag gccatagaaa agctggaaga gaagctccag
     1081 gcccagtctg actatgagga aattaaaacg gagctgagca tcctgaaagc catgaagctg
     1141 gcctccagca cctgcagcct cccccagggc atggccaagc ctgaagactc actgcttatt
     1201 gcaaaggagg ccttcttccc cacgcagaaa ttccttctgg agaagcccag cctcctggcc
     1261 agccctgagg aagacccatc agaggacgat tccatcaagg attcactggg cacggagcag
     1321 tcctacccct cccctcagca gctcccacct ccaccagggc cagaagaccc cctgtctccc
     1381 agccccgggc agcccctgct gggccccagc ttggggcctg acggcactcg gactttctcg
     1441 ctgtccccct tccccagcct ggcatcaggg gagagactga tgatgccccc agccgccttc
     1501 aagggagagg cgggcggcct gctggtgttc cccccagcct tctatggcgc caagcccccc
     1561 acagcccctg ccaccccggc ccctggccct gagccactgg gcggtcctga gcccgcggat
     1621 ggtggtgggg gcggagcggc ggggcccggg gcagaggagg agcagctgga cacggcagag
     1681 atcgccttcc aggtgaagga gcagctgctg aaacacaaca tcgggcagcg ggtgtttggg
     1741 cattacgtgc tggggctgtc gcagggctcg gtcagcgaga tcctagcccg gcccaagccc
     1801 tggcgcaagc tcacggtgaa gggcaaggag cccttcatca agatgaagca gttcctgtcg
     1861 gatgagcaga atgtactggc gctcaggacc atccaagtgc ggcagcgagg cagcatcacc
     1921 ccgagaatcc gcacgcctga gacaggctca gacgacgcca tcaagagcat tctagagcag
     1981 gccaagaagg agatcgagtc gcagaagggc ggcgagccca agacctcggt ggccccgctg
     2041 agcatcgcca acggcacgac ccccgccagc acctcggagg acgccatcaa gagcatcctg
     2101 gagcaggcac gccgtgagat gcaggcgcaa cagcaggcgc tgctggagat ggaggtggcg
     2161 cccaggggcc gctcggtgcc cccctcgccc ccggagcggc catcactggc caccgcgagc
     2221 cagaacgggg ccccggcctt ggtgaagcag gaggagggca gcgggggccc cgcgcaggcg
     2281 ccgctcccgg tcctgtcccc cgccgccttc gtgcagagca tcatccgcaa ggtcaagtcc
     2341 gagatcggcg acgccggcta cttcgaccac cactgggcct ccgaccgcgg cctgctcagc
     2401 cgcccctacg cctccgtgtc gccctcgctg tcctcctcct cctcctctgg ctactctggc
     2461 cagcccaacg gccgcgcctg gccccgcggg gacgaggccc ctgtgccccc cgaggacgag
     2521 gcggcggcag gggcggagga cgaacccccc aggacgggcg agctcaaggc tgagggcgcg
     2581 acggccgagg cgggcgcgcg gctgccctac tacccggcct acgtgccgcg caccctgaag
     2641 cccaccgtgc cgccgctgac ccccgagcag tacgagctgt acatgtaccg tgaggtagac
     2701 acgctggagc tcacccgcca ggtcaaggag aagctggcca agaacggcat ctgccagagg
     2761 atcttcgggg agaaggtgct gggcctgtca cagggcagcg tgagcgacat gctgtcccgg
     2821 ccgaagccat ggagcaagct gacgcagaag gggcgggagc ccttcatccg catgcagctg
     2881 tggctctctg accagctcgg ccaggcagtg ggccagcagc ctggtgcctc ccaggccagt
     2941 cccacagaac caaggtcctc accatcccca ccccccagcc ccacagagcc tgagaagagc
     3001 tcccaggagc cgttgagcct gtccctggag agcagcaagg agaaccagca gccagagggc
     3061 cgctccagct cctcgttgag cgggaagatg tactcaggca gccaggcccc agggggcatc
     3121 caggagatcg tggccatgtc ccccgagctg gacacgtact ccatcaccaa gagggtgaag
     3181 gaggtcctca cagacaacaa tctagggcag cggctgtttg gggaaagcat cctgggtctg
     3241 acacagggct ccgtgtctga cctgctgtcc cggcccaaac cctggcacaa gctgagcctg
     3301 aaggggcggg agccttttgt ccgcatgcag ctgtggctca atgaccccca taacgtggag
     3361 aagctgaggg atatgaagaa gctggagaag aaagcctacc tgaaacgtcg ctatggcctc
     3421 atcagcaccg gctcagacag tgagtccccg gccacccgct cagagtgccc cagcccctgc
     3481 ctgcagcccc aggacctgag cctcctgcag atcaagaagc cccgggtggt gctggcaccc
     3541 gaggagaagg aggcactgcg gaaggcctat cagctggaac cctacccctc gcagcagacc
     3601 atcgagctcc tctccttcca gctcaacctc aagaccaaca ccgtcatcaa ctggttccac
     3661 aactacaggt cccggatgcg ccgggagatg ttggtggagg ggacccagga tgagccagac
     3721 cttgatccaa gcgggggtcc tggaatccta ccgccaggcc actcccaccc agaccccacc
     3781 ccgcagagcc ctgactctga gactgaggac cagaagccaa ccgtgaagga actggagctt
     3841 caggagggcc ctgaggagaa cagcacaccc ctgaccaccc aggacaaggc ccaagtgagg
     3901 atcaagcagg aacagatgga ggaggatgct gaggaagagg caggcagcca gccccaggac
     3961 tcaggggagc tggacaaagg ccaaggtccc cccaaagagg agcatcccga ccctccgggt
     4021 aatgatggac tcccaaaagt ggctcccggg cccctccttc caggtggatc caccccagac
     4081 tgtccctcac ttcatcccca acaggagagt gaggccgggg agcgacttca cccggaccct
     4141 ttaagtttta agtcagcctc agagtcctca cgctgcagcc tggaggtgtc actgaactcg
     4201 ccctcggccg cctcctcacc aggcctcatg atgtctgtgt cacctgtccc ctcctcctca
     4261 gctcccatct ccccatcccc acctggcgcc ccccctgcca aagtgccgag tgccagcccc
     4321 actgctgaca tggctggagc cttgcacccc agtgccaagg tgaaccccaa cttgcagcgg
     4381 cggcatgaga agatggccaa tctgaacaac atcatttacc gagtagagcg ggctgccaat
     4441 cgggaggagg ccctggagtg ggagttctag taatgaacta gtccgtaact tgaaagtatt
     4501 tcgatttctt ggctttatat atcttgtgga aaggacgact aggactagag aggtcgcgga
     4561 aa
//
