LOCUS       sequence-282485-        5135 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 5135)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 5135)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0912
FEATURES             Location/Qualifiers
     source          1..5135
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggaggttcc agaacccacc tgccctgccc ctcctgcgag ggaccagcca
       61 gctcccactc ctggccctcc aggggcccca ggtggccagg cctcacctca cctgaccctg
      121 ggccctgtcc ttctgccgcc agagcagggc ctggcccccc ccactgtgtt cctgaaggcc
      181 ctgcccatcc cactgtacca cacggtgcct cccgggggcc tccagccccg cgccccgcta
      241 gtgacgggca gcctagatgg gggcaacgtg cccttcatac tcagccctgt gctgcagcct
      301 gaagggcctg gccccaccca ggtggggaag ccggcggccc ctacgctgac ggtgaacatc
      361 gtgggcactc tgcctgtcct gtcgccgggc ctgggcccca cgctgggcag cccaggcaag
      421 gtgcggaatg cgggcaagta cctgtgtccg cactgtggtc gcgactgcct gaagcccagt
      481 gttctagaga agcacatccg gtcccacacg ggtgagaggc ccttcccgtg tgccacctgc
      541 ggcatcgcct ttaagaccca gagcaatctc tacaagcaca ggcggacgca gacgcacctc
      601 aacaactccc ggctgtcctc agagtccgag ggcgccgggg gcggcctcct ggaggaaggg
      661 gacaaggccg gagagccccc cagaccagag ggcaggggcg agagcaggtg ccaggggatg
      721 cacgaaggcg cctcggagag acccctttct ccgggtgccc acgtgcccct acttgccaag
      781 aacctggatg tgaggaccga agctgctccc tgtccagggt ccgcatttgc cgacagagag
      841 gctccttggg actctgcccc catggcgtca cctgggctcc cagcggccag cacacaaccc
      901 tggcgtaagt tgccagagca gaagtcgccg accgccggga agccgtgcgc cctgcagcgg
      961 cagcaggcga cggcagcgga gaagccctgg gatgccaagg cccccgaggg ccggctgcgg
     1021 aagtgtgaga gcaccgactc ggggtacctg tcgcgctccg acagcgcgga gcagccgcat
     1081 gcgccctgca gccccctgca cagcctttcg gagcacagcg ccgagtccga gggggagggc
     1141 ggcccgggcc cggggccagg ggtcgcaggg gccgagcccg gggcgcgaga agccggcctg
     1201 gagctggaga agaagcggct ggaggagcgc atcgcccagc tcatctccca caaccaggcg
     1261 gtggtggacg atgcccagct ggacaacgtg cggccccgga agaccgggct gtccaaacag
     1321 ggcagcatcg acctgcccac gccctacacc tacaaggact ccttccactt tgacatccgc
     1381 gcgctggagc caggccgtag gagggccccg ggccccgtgc gctccacctg gacgccccca
     1441 gacaagtctc ggcccctctt cttccactcc gtccccactc agctctccac caccgtggaa
     1501 tgtgtccccg tcaccaggag caactcgctg cccttcgtcg agggctccag gacgtggctg
     1561 gagcccaggg agccccggga cccctggtcc aggacgcaga agcctctgag ccccaggccc
     1621 ggcccagccc gcctgggctg ccgctcggga ctaagctcga ctgacgttcc cagtgggcat
     1681 ccccgggccc tggtcagaca ggccgcggtg gaggacctgc caggcacccc cattggcgat
     1741 gccctggtgc ccgcagagga cacagacgca aagagaactg ctgcgcggga ggccatggcc
     1801 ggcaagggca gagcgggcgg caggaagtgc ggccagagaa ggctgaagat gttctcccag
     1861 gagaagtggc aggtgtacgg ggatgagacg ttcaaaagga tctaccagaa aatgaaagcc
     1921 agtccccatg gaggcaagaa agccagggag gtgggaatgg gcagtggggc agaactgggc
     1981 tttcctctgc agaaagaggc agcagggagc tcaggcacag tccccaccca agacaggagg
     2041 acccctgtcc atgaggacat atccgcaggg gcaacgccag agccttgggg aaatccacca
     2101 gccctggagg cctccttggt gactgaaccc actaagcatg gggagacggt ggccaggaga
     2161 ggagacagtg accgacccag ggtggaagag gctgtgtcat cccctgcact gggtggcaga
     2221 gacagtccct gttcaggcag taggagcccc ctggtctctc caaatgggag gctggaactg
     2281 gggtggcaga tgcccccagc acctggcccc ctcaaagggg gtgatgtaga ggctcccagg
     2341 ccagtttggc cggaccccaa gctggaagga ggtgcccgag gtgtggggga tgttcaggag
     2401 acctgcctgt gggcccagac tgtcctgaga tggcccagca ggggctcagg ggaggacaag
     2461 ctcccctcag agaggaagaa gctgaaagtg gaggacctgc acagctggaa gcaaccagag
     2521 cctgtgagcg cagagacccc aggtgggccc acgcagcctg cctctttgtc atcccagaag
     2581 caggatgccg atcccgggga ggtgccaggg ggctcaaagg agagtgccag gcaggtgggc
     2641 gagcctctgg agtcctctgg agcctccttg gctgctgctt ctgttgccct gaagagggtg
     2701 gggccaaggg acaaggctac cccactgcat cctgcagccc cagcccccgc agagcacccc
     2761 tcgctggcca ccccacctca ggctcctaga gtgctctctg ccctggcaga taatgccttt
     2821 tcccccaagt acctcctcag gttacctcag gcagagaccc ccttaccact gcccattccc
     2881 tggggaccaa ggcacagcca ggactctctc tgcagcagtg ggtggcctga agaacgggca
     2941 tcatttgttg ggtcaggact ggggacccct ctttctccca gcccagcctc aggcccctcc
     3001 ccaggtgagg cggacagcat cctggaggac cccagctgtt ccaggccaca ggatgggaga
     3061 aaaggggcac agttgggggg ggacaagggg gacaggatgg ccactagcag gccagcagcc
     3121 agggagttgc ccatctcagc accaggggct cccagggagg ctacctcctc cccgcccact
     3181 ccaacgtgtg aggcacactt agttcaggac atggagggtg acagccaccg tatccatcgc
     3241 ctctgcatgg gcagcacttt ggcaagggcc aggctctctg gggatgtcct gaatccctgg
     3301 gtacccaact gggagctggg ggagcctcct gggaatgccc cagaagatcc ttcttcaggg
     3361 cccctggtgg gccccgaccc gtgttccccc ctccagcctg gctccttcct cactgccctc
     3421 actcggcctc agggtgtgcc cccaggctgg ccagagctgg ccttgtcttc ccactcaggg
     3481 acgtcccgga gccacagcac ccgcagtccc cacagcaccc aaaacccctt tccctcactg
     3541 aaggctgagc cgcggctcac gtggtgttgc ctgagccgca gtgtccctct gcccgcggag
     3601 cagaaggcaa aggcggcatc tgtgtacttg gcggtgcact ttcctggtag cagcctccga
     3661 gatgagggtc ccaatggccc tcctgggagc aatggaggat ggacctggac aagccctgga
     3721 gaaggagggc cggcgcagat gtccaagttc tcctacccaa cagtcccagg ggtgatgccc
     3781 cagcaccagg tgtctgagcc agaatggaag aaaggcctgc cttggagggc aaagatgtct
     3841 cgtgggaaca gcaagcagag aaaactgaag atcaacccta aaaggtacaa agggaatttc
     3901 ttgcagagct gtgttcagct gagagccagt agacttcgca caccaacctg ggtgcgaaga
     3961 agaagccgcc accctcccgc acttgaggga ctgaagccat gcaggacccc tgggcagacc
     4021 tcttcagaaa tagcaggtct gaatctgcaa gaggagccat cttgtgccac ctcagaatca
     4081 cctccttgtt gtgggaagga agagaagaag gaaggtgact gcagacaaac cttaggaacc
     4141 ctctctcttg gtacaagttc aagaattgtc agggaaatgg acaaacgaac tgtgaaggat
     4201 atttctccat ctgctggtga gcatggtgac tgtactactc acagcactgc tgccacatca
     4261 ggattatctc tgcaatctga cacctgcctg gcagtggtta atgacgtgcc tctaccccct
     4321 ggcaaaggtc ttgaccttgg gttgctggag actcagctgc tggcctccca ggattcagtc
     4381 tcaacagatc ccaaaccata catcttctca gatgctcaaa ggccttcttc ctttgggtcc
     4441 aaaggaactt ttccccacca tgacattgct acctctgtgg ctgccgtttg tatttctctg
     4501 ccagtgagaa cagatcacat agcccaggaa attcacagtg ctgaatcacg agaccacagc
     4561 cagactgcag ggaggactct gacatcaagc tccccagaca gcaaagtcac agaagagggc
     4621 agagcacaga ccctcttgcc agggagacct tcatctggac aaagaatttc agattcggtt
     4681 ccactggagt caactgaaaa aactcatctt gaaataccag cttcaggacc aagttcagct
     4741 agttcacacc acaaggaagg gagacacaag acgttttttc cttccagagg ccagtatggg
     4801 tgtggggaaa tgactgtccc ctgcccctct ttaggaagtg acggtaggaa acgtcaggta
     4861 tctggattaa tcactcggaa agattctgtg gttccttcta agccagagca gcccatagaa
     4921 attcctgaag ccccttctaa atccctcaag aagaggagtc tggaaggaat gagaaagcaa
     4981 actcgagtag agttcagtga caccagcagc gacgatgaag accgattagt tatagaaata
     5041 tagtaatgaa ctagtccgta acttgaaagt atttcgattt cttggcttta tatatcttgt
     5101 ggaaaggacg acacccatat ccctacgata gcagc
//
