LOCUS       sequence-282488-        2912 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2912)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2912)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0914
FEATURES             Location/Qualifiers
     source          1..2912
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggctcttac acagggacct ttgacattca gggatgtagc catagaattc
       61 tctcaggagg agtggaaatc cctggaccct gtgcagaaag ctttgtactg ggatgtgatg
      121 ttggagaact acaggaacct ggtcttcctg ggtatccttc ctaaatgtat gaccaaggaa
      181 ttaccaccaa tagggaacag taatacagga gaaaaatgcc aaacagtgac gctggaaaga
      241 catgaatgtt atgatgttga aaatttttac ttaagggaaa tccagaaaaa tctacaggac
      301 cttgagtttc aatggaaaga tggtgaaata aattataaag aagtgccaat gacctataaa
      361 aacaatctta atggtaaaag aggtcaacat agtcaagagg atgtagaaaa caaatgtatt
      421 gaaaatcagc ttacattaag ctttcagtca cgtctgactg aactgcagaa atttcaaact
      481 gaagggaaaa tttatgagtg taaccaatct gagaagacag ttaataacag ttccctagtt
      541 tcaccacttc aaagaattct tcctagtgtc caaaccaaca tttctaaaaa atatgagaat
      601 gagtttttgc agctgtcatt acccacccaa cttgagaaaa cacacattag ggaaaaacct
      661 tatatgtgta aagggtgtgg caaagccttt agagtgtctt caagtcttat taaccatcag
      721 atggtacata ctacagagaa accttacaaa tgcaatgaat gtggcaaagc ctttcatcgg
      781 ggctcactac taactataca tcagatagtc catacaaggg ggaagccata tcaatgtggt
      841 gtatgtggca agatcttcag acaaaattca gatcttgtaa atcaccggag aagtcacact
      901 ggagagaaac cgtacaaatg taatgaatgt ggcaagtcct ttagtcaaag ttataacctt
      961 gcaatacatc agagaattca cactggagag aaaccttaca aatgtaatga gtgtgggaaa
     1021 accttcaaac aaggctcatg cctcactaca catcagataa tccatacagg agagaaacca
     1081 tatcaatgtg atatatgtgg caaggtcttc aggcagaatt ctaatcttgt aaatcaccag
     1141 agaatccaca ctggagagaa accatacaaa tgcaacatat gtggaaagtc ctttagtcaa
     1201 agttccaacc tggcaactca tcagacagtt catagtggaa acaaacctta caaatgtgat
     1261 gagtgtggca aaacctttaa acggagctcc agcctcacta cacatcagat aatccataca
     1321 ggagagaaac catatacatg tgatgtatgc gacaaggtct tcagtcaacg ttcacaactt
     1381 gcaaggcacc agagaagtca tactggagag aaaccttaca aatgcaatga atgtggcaag
     1441 gtcttcagtc agacttcaca tcttgtgggg catcggagaa ttcatactgg agagaaacct
     1501 tacaaatgtg ataaatgtgg taaagccttt aaacagggct cattactcac tcgacataag
     1561 ataattcata ccagagagaa acgttaccaa tgcggtgaat gtggaaaggt ctttagtgaa
     1621 aattcatgcc ttgtaagaca tttaagaatt catactgggg agcaacctta caaatgtaat
     1681 gtgtgtggca aggtcttcaa ttacagtgga aacctttcaa ttcataagag aatacatacg
     1741 ggagagaaac ctttccaatg taatgaatgt ggcacagtct tcaggaacta ctcatgccta
     1801 gcacgtcatc taagaattca tactgggcag aaaccttaca aatgtaatgt gtgtggcaag
     1861 gtcttcaatg acagtggaaa cctttcaaat cataagagaa ttcatactgg agagaagccg
     1921 tttcaatgta acgaatgcgg caaggttttc agttactact catgcctagc acgtcatcgg
     1981 aaaattcata ccggagagaa accttacaaa tgtaatgatt gtggcaaagc ctatactcag
     2041 cgttcaagcc tcactaaaca tctgataatt catactggag agaaacctta caattgtaat
     2101 gagtttggag gggcatttat ccaaagttca aaacttgcaa gatatcacag aaatcctact
     2161 ggggagaaac cacacaaatg tagccattgt ggtagaactt ttagtcatat aacaggcctg
     2221 acgtaccatc agagaaggca tactggagag atgccataca aatgtattga atgtggccag
     2281 gtctttaatt ccacttcgaa ccttgcaagg catcggagaa ttcacactgg agagaaacct
     2341 tacaaatgta atgaatgtgg caaggtcttt cgtcatcaat caacactagc acgtcatcgg
     2401 agtattcata ctggagagaa accttacgtg tgtaatgagt gtggcaaagc ctttagagtg
     2461 cgttcaattc tggttaatca tcagaaaatg catactgggg acaaacctta caaatgtaat
     2521 gaatgtggta aagcttttat tgaaaggtca aagctggtgt accatcaaag aaatcacact
     2581 ggagagaagc catacaaatg tattgaatgt ggcaaggcct ttgggcggtt ttcttgcctc
     2641 aacaaacacc aaatgattca ttctggagaa aaaccttata aatgtaatga gtgtggcaaa
     2701 tctttcatta gtcgctcagg cctcactaaa catcagacaa aacatactgc agagagtctt
     2761 aaaacaaaat tcaatgtgga aaagccttta gatgttctcc taacttctgg gttcaaatag
     2821 taatgaacta gtccgtaact tgaaagtatt tcgatttctt ggctttatat atcttgtgga
     2881 aaggacgagc ggcacacgct cctgcatgga ca
//
