LOCUS       sequence-282500-        1598 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1598)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1598)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0957
FEATURES             Location/Qualifiers
     source          1..1598
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggagtcccc aagagggtgg accctgcagg tggccccaga ggaaggccag
       61 gtcctctgca atgtgaagac tgccacgagg ggcctctctg agggggctgt gtctggaggc
      121 tggggggcct gggaaaactc cacggaggtt ccgagggagg caggggacgg ccagcggcag
      181 caagccacac tgggggcggc ggacgaacag ggaggccccg gcagggagct gggccccgca
      241 gacggtgggc gggacggggc tgggcccagg agcgagcctg cagaccgggc gttgcgccct
      301 tcgcctctcc cagaggagcc gggctgccgg tgcggggagt gcggcaaggc gttcagccag
      361 ggctcttact tgctgcagca tcggcgcgtg cacacaggcg agaagccgta cacgtgcccc
      421 gagtgcggca aggccttcgc ctggagctcc aacctcagcc agcaccagcg catccacagc
      481 ggcgagaagc cctacgcttg cagggagtgc ggcaaggcct tccgcgcgca ctcgcagctc
      541 atccaccacc aggagacaca cagcggcctg aagcccttcc gctgcccgga ctgcggcaag
      601 tccttcggcc gaagcaccac gctggtgcag caccgacgca cgcacacggg cgagaagccc
      661 tacgagtgcc cggagtgcgg caaggccttc agctggaact ccaatttcct ggagcaccgg
      721 cgcgtgcaca cgggcgcgcg gccgcacgcc tgccgggact gtggcaaggc cttcagccag
      781 agctccaacc tggccgagca cctgaagatc cacgcgggcg cacggccaca cgcctgtccc
      841 gactgcggca aggccttcgt gcgtgtggcg gggctgcggc agcaccggcg cacgcacagc
      901 agcgagaagc ccttcccctg cgccgagtgc ggaaaggctt tccgcgagag ctcgcagctc
      961 ctgcagcacc agcgcacgca cactggtgag cggcccttcg agtgcgccga gtgcggccag
     1021 gctttcgtca tgggctccta cctggcggag caccggcgcg tgcacacggg cgagaagcct
     1081 catgcgtgcg cccagtgcgg caaggccttc agccagcgct ccaacctact gagccaccgg
     1141 cgcacgcact cgggcgccaa gcccttcgcc tgcgccgact gcggcaaggc cttccgcggc
     1201 agttccggcc tggcgcacca ccggctttcg cacacgggag agcgaccctt cgcctgcgca
     1261 gaatgcggca aggccttccg cggcagctcc gagctgcgcc agcaccagcg cctgcactct
     1321 ggcgagaggc cgttcgtctg cgcccactgc agcaaggcct tcgtgcgcaa gtcggagctc
     1381 ttaagccacc ggcgcacgca cacgggcgag aggccctacg cttgcggcga gtgcgggaag
     1441 cctttcagcc accgttgcaa cctcaacgag caccagaagc ggcacggggg ccgcgctgcg
     1501 ccctagtaat gaactagtcc gtaacttgaa agtatttcga tttcttggct ttatatatct
     1561 tgtggaaagg acgagacatt tggcgccgcc atgtagta
//
