LOCUS       sequence-282503-        1190 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1190)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1190)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0988
FEATURES             Location/Qualifiers
     source          1..1190
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggccacagc cgagaccgcc ttgccctcca tcagcacact gaccgccctg
       61 ggccccttcc cggacacaca ggatgacttc ctcaagtggt ggcgctccga agaggcgcag
      121 gacatgggcc cgggtcctcc tgaccccacg gagccgcccc tccacgtgaa gtctgaggac
      181 cagcccgggg aggaagagga cgatgagagg ggcgcggacg ccacctggga cctggatctc
      241 ctcctcacca acttctcggg cccggagccc ggtggcgcgc cccagacctg cgctctggcg
      301 cccagcgagg cctccggggc gcaatatccg ccgccgcccg agactctggg cgcatatgct
      361 ggcggcccgg ggctggtggc tgggcttttg ggttcggagg atcactcggg ttgggtgcgc
      421 cctgccctgc gagcccgggc tcccgacgcc ttcgtgggcc cagccctggc tccagccccg
      481 gcccccgagc ccaaggcgct ggcgctgcaa ccggtgtacc cggggcccgg cgccggctcc
      541 tcgggtggct acttcccgcg gaccgggctt tcagtgcctg cggcgtcggg cgccccctac
      601 gggctactgt ccgggtaccc cgcgatgtac ccggcgcctc agtaccaagg gcacttccag
      661 ctcttccgcg ggctccaggg acccgcgccc ggtcccgcca cgtccccctc cttcctgagt
      721 tgtttgggac ccgggacggt gggcactgga ctcgggggga ctgcagagga tccaggtgtg
      781 atagccgaga ccgcgccatc caagcgaggc cgacgttcgt gggcgcgcaa gaggcaggca
      841 gcgcacacgt gcgcgcaccc gggttgcggc aagagctaca ccaagagctc ccacctgaag
      901 gcgcatctgc gcacgcacac aggggagaag ccatacgcct gcacgtggga aggctgcggc
      961 tggagattcg cgcgctcgga cgagctgacc cgccactacc ggaaacacac ggggcagcgc
     1021 cccttccgct gccagctctg cccacgtgct ttttcgcgct ctgaccacct ggccttgcac
     1081 atgaagcgcc acctttagta atgaactagt ccgtaacttg aaagtatttc gatttcttgg
     1141 ctttatatat cttgtggaaa ggacgatcca gaatagttga ctaagctcat
//
