LOCUS       sequence-282506-        2222 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2222)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2222)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0995
FEATURES             Location/Qualifiers
     source          1..2222
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcagcggc ggtgctgatg gaccgggttc agagttgtgt gaccttcgag
       61 gatgtgttcg tgtacttctc tcgggaggag tgggaactcc ttgaggaggc acagagattc
      121 ctgtaccgtg atgtgatgct ggagaacttt gcacttgtgg ctacactagc tttcattttc
      181 ccagtcccat gcagttgcgc agttggaggg ggcagaaaac cttgggtgcc tgacagggca
      241 gacatcactg cagccacagt aaaggagacc tacagagggc atggccctgc tgaatgggag
      301 ctggaagagg gtttttggtg tgaagcagaa catgaggcac cttctgagca gagcgtttct
      361 gtagaaggag tgtcacaggt caggactgct gagtcaggtc ttttccagaa agcacaccca
      421 tgtgagatgt gtgacccact cttgaaagac attttgcacc tggctgaaca ccagggatca
      481 caccttacac agaaactgtg cacacgtggg ctgtgtagga gaagattctc gttcagtgca
      541 aacttttacc agcaccagaa gcaacataat ggagagaatt gcttcagagg ggatgatgga
      601 ggggcctcat ttgtgaagag ctgtacagtc cacatgttag ggagatcctt tacgtgcagg
      661 gaggaaggga tggacttacc agatagctct ggccttttcc agcaccagac cacttacaat
      721 agggtgagtc catgcagaag gactgaatgc atggagtctt tcccacacag ctccagtctc
      781 aggcaacacc aaggagacta tgatggacag atgcttttca gttgcggtga tgaagggaaa
      841 gccttcctgg acacctttac tcttcttgac agccagatga ctcatgctga ggtgagaccc
      901 ttcagatgcc taccatgtgg aaatgtgttc aaggagaaat cagctcttat taatcacaga
      961 aaaatccaca gtggagaaat atctcatgtg tgtaaggagt gtggaaaagc cttcattcac
     1021 ttgcaccacc taaaaatgca ccagaaattt cacactggaa aaagacacta tacatgcagt
     1081 gaatgtggga aggccttcag ccgcaaggac acacttgttc agcatcagag agttcacact
     1141 ggagaaagat cttatgactg cagtgaatgt ggaaaagcct acagcagaag ctcccacctt
     1201 gttcagcacc agagaattca cacaggagaa aggccttata agtgcaacaa atgtgggaaa
     1261 gcctttagcc gtaaagacac acttgttcag caccagagat ttcatactgg agaaaggcct
     1321 tatgagtgca gtgaatgtgg aaaattcttt agccaaagct cccaccttat tgagcactgg
     1381 agaattcata ccggggcaag gccctatgaa tgcatagaat gtggaaaatt ctttagccat
     1441 aactctagcc tcattaaaca tcggagagtc cacacaggag caagatccta cgtgtgcagc
     1501 aaatgtggga aggcctttgg ctgcaaagac acacttgttc agcaccagat aattcacact
     1561 ggagcaaggc cttatgagtg cagtgaatgt gggaaggcct tcagccgtaa agacacactt
     1621 gtgcaacacc aaaaaatcca cactggagaa aggccttatg agtgtggtga atgtggtaaa
     1681 ttcttcagcc atagctccaa ccttattgta caccagagaa ttcacactgg agcaaagcct
     1741 tatgagtgca atgaatgtgg gaaatgcttt agccacaact ccagcctcat tttgcaccag
     1801 agagttcaca caggagcaag gccttatgtg tgcagtgaat gtgggaaggc ttacattagt
     1861 agctcccacc ttgttcaaca caagaaagtt cacactggag caagacctta tgagtgcagt
     1921 gaatgtggga aattctttag ccgcaactct ggcctcattc tgcaccagag ggttcacact
     1981 ggagaaaagc cttacgtatg cagcgaatgt gggaaagcct atagcagaag ctcccatctt
     2041 gttcgtcacc agaaagctca cactggagaa agagctcacg agtgcaacag ttttggtggc
     2101 cctttagctg catctcttaa acttgtttag taatgaacta gtccgtaact tgaaagtatt
     2161 tcgatttctt ggctttatat atcttgtgga aaggacgagg ctcgagccct tctgagcgaa
     2221 gt
//
