LOCUS       sequence-282509-        1763 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1763)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1763)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1006
FEATURES             Location/Qualifiers
     source          1..1763
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggactcagt ggcctctgag gatgtggctg tgaacttcac acttgaggag
       61 tgggctttgc tggatccttc ccagaagaaa ctctacagag atgtgatgcg ggaaaccttt
      121 agaaacctgg cctgtgtagg aaaaaaatgg gaagaccaga gcattgaaga ttggtacaaa
      181 aatcagggga gaattttaag aaatcatatg gaagagggac tcagtgaaag taaagaatat
      241 gatcaatgtg gagaagcctt cagtcagatt ctcaatctta atctgaacaa gaaaattcct
      301 actatagtaa gaccatgtga atgtagtttg tgtgggaaag tcttcatgca tcattcatcc
      361 cttagtaggc acatcagatc tcaccttgga cacaaaccat atgactatca ggaatatgga
      421 gagaaaccat ataaatgtaa gcagtgtggg aaagccttca gttcttgtca atcctttcga
      481 agacatgaaa gaactcacac tggtgagaaa ccctatgcat gtccggaatg tgggaaagcc
      541 ttcatttctc tcccaagtgt tcgaagacac atgattaagc acactggaga tggaccatat
      601 aaatgtcagg aatgtgggaa agcctttgat cgcccaagtt tatttcagat acatgaaaga
      661 actcacactg gagagaaacc ctatgaatgt caggaatgtg caaaagcttt catttctctt
      721 ccaagttttc aaagacacat gattaggcac actggagatg gaccttataa atgtcaggaa
      781 tgtggaaaag cctttgaccg ccccagttta tttcgaatac atgaaagaac tcatactgga
      841 gagaaacccc atgaatgtaa acagtgtggg aaggccttca tttctttcac aaattttcaa
      901 agtcatatga ttaggcacac tggggatgga ccttataaat gtaaagtatg tgggagagcc
      961 tttatttttc ccagttatgt tcgaaagcat gaaagaactc atactgggga gaaaccctat
     1021 gaatgtaata aatgtggtaa aaccttcagt tcttccagta atgttcgaac acatgaaagg
     1081 actcacactg gagagaagcc ctatgaatgt aaggaatgcg ggaaagcctt catttctctc
     1141 ccaagtgtcc gaagacacat gataaagcac actggagatg gaccttataa atgtcaggta
     1201 tgtggtagag cctttgactg tcccagttca tttcaaatac acgaaagaac tcacactgga
     1261 gagaaaccct atgaatgtca ggtatgtggg aaagccttca tttctcttaa aaggattaga
     1321 aaacatatga tactgcacac tggagatgga ccttataaat gtcaggtatg tggtaaagcc
     1381 tttgactgtc ctagttctgt ccgaacacat gaaagaactc atactggaga aaaaccctat
     1441 gaatgtaaag aatgtgggaa agcattcaat tatgccagtt ccattagaat acatgaaaga
     1501 actcataccg gagaaaaacc ctatgaatgt aagcaatgtg gaaaaacgtt cagttattcc
     1561 agttcctttc aaagacatga aagagctcat aatggagata aaccttacgt aaagaatgtg
     1621 ggaaagcttt catttatcac acaaccttcg aatacctgtg aaaatgaata gtaatgaact
     1681 agtccgtaac ttgaaagtat ttcgatttct tggctttata tatcttgtgg aaaggacgac
     1741 gatgctattt gcgaggaggt agc
//
