LOCUS       sequence-282512-        1058 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1058)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1058)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1025
FEATURES             Location/Qualifiers
     source          1..1058
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggccaccta ctgcgacgac ctgggcccct cctcggcccc gcccggccag
       61 gcccaggcca ccgcgcaccc cccgggctat gagccagggg atctgggcgc ggtgggcggg
      121 ggccccctcc tgtgggtgaa cgcgccagcg ctcagcccca agtcctacgc ttcgggtccc
      181 gggcctgcgc cgccctacgc ggccccgagc tacggggctc ccggcccgct cctcggcgcc
      241 ccgggcggcc tggcgggcgc cgacctcgcc tggctgagcc tctccggcca gcaggagctg
      301 ctgaggctgg tgcggccgcc ctactcctac tcggcgctca tcgccatggc catccagagc
      361 gcgccgctgc ggaagctgac gctcagccag atctaccagt acgtggctgg taacttccct
      421 ttctacaagc gcagcaaggc gggctggcag aactccatcc gccacaacct gtcgctcaac
      481 gactgcttca agaaggtgcc ccgcgacgag gacgacccag gtaaaggcaa ttactggacc
      541 ctggacccca actgcgagaa gatgtttgac aacgggaact tccgaaggaa gaggaagagg
      601 agagctgaag ccagcgcggc cgtgcgctcg ggagccagga gcgtgggagg ggccgaggcg
      661 ccagcgctgg agcccccgag cgcggcttgc ctggacctgc aggcctcgcc ctctccatcc
      721 gcacccgagg ccgccacctg cttctccggt ttcgcttctg ctatgagcgc tctggctggc
      781 ggccttggca ccttccccgg gggcctggcg ggcgactttt ctttcgggag gcggccaccg
      841 acagtcgcca cccacgctcc ccagaccctt aacccctccc ctggcttcgc ccctggccac
      901 cagaccgcgg ccgccggctt ccgcctcagt cacctcctct acagccggga agggaccgaa
      961 gtttagtaat gaactagtcc gtaacttgaa agtatttcga tttcttggct ttatatatct
     1021 tgtggaaagg acgattcagc gggactctcc ggcagcta
//
