LOCUS       sequence-282530-        1394 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1394)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1394)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1084
FEATURES             Location/Qualifiers
     source          1..1394
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggccagctc tgctaagtca gctgagatgc ccaccatctc caaaaccgtt
       61 aaccctactc ctgatcctca tcaagaatat ctggacccta ggattaccat tgccttattc
      121 gaaattggat cacattcccc ttcctcctgg ggctctctcc ctttcctaaa gaatagcagc
      181 catcaagtta cagaacaaca gactgcacag aagtttaaca atctcttaaa agaaattaaa
      241 gatattctta aaaatatggc aggttttgaa gagaagatca cagaagcaaa agaacttttt
      301 gaggaaacca atattactga ggatgtgtca gcccacaaag aaaatatcag aggacttgac
      361 aaaatcaatg aaatgttatc aacaaacctg cctgttagtt tagccccaga gaaagaagac
      421 aatgaaaaga aacaggagat gatattggaa accaatatta ctgaggatgt gtcagcccac
      481 aaagaaaata tcagaggact tgacaaaatc aatgaaatgt tatcaacaaa cctgcctgtt
      541 agtttagccc cagagaaaga agacaatgaa aagaaacagc agatgataat ggaaaaccag
      601 aactctgaga acaccgcaca agtttttgca agagatttgg taaatcgttt agaagaaaaa
      661 aaagtcctta acgaaactca acaaagtcag gaaaaagcaa aaaacagact taatgttcaa
      721 gaagaaacta tgaaaattag gaacaacatg gagcagttac tacaggaagc agaacactgg
      781 agtaaacaac atactgagct cagtaaactg ataaaatcct atcagaaatc tcagaaagac
      841 ataagtgaaa ctcttggaaa taatggagtc ggtttccaaa cccagccaaa taatgaagtg
      901 tcggctaagc atgagctgga ggaacaggtg aagaaactga gccatgacac ctattcattg
      961 cagttgatgg cagctttgct agagaatgaa tgccaaatct tacagcagag agtagagatt
     1021 ctcaaggaac tccatcatca gaaacaggga actctgcaag agaagccaat tcagataaac
     1081 tataaacagg acaagaaaaa tcagaagcca tcagaagcaa agaaagtaga aatgtataag
     1141 cagaacaagc aagcaatgaa gggtacattt tggaaaaaag acagatcctg tagaagcctg
     1201 gatgtttgtc ttaataagaa agcttgcaat acccagttca atattcatgt tgcaagaaaa
     1261 gctcttaggg gaaaaatgag gtcagctagc agcctaagat agtaatgaac tagtccgtaa
     1321 cttgaaagta tttcgatttc ttggctttat atatcttgtg gaaaggacga cgttatatgg
     1381 gtgcggttat aaac
//
