LOCUS       sequence-282536-        7316 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 7316)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 7316)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1109
FEATURES             Location/Qualifiers
     source          1..7316
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      4762..4771
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggctgagaa cttgctggac ggaccgccca accccaaaag agccaaactc
       61 agctcgcccg gtttctcggc gaatgacagc acagattttg gatcattgtt tgacttggaa
      121 aatgatcttc ctgatgagct gatacccaat ggaggagaat taggcctttt aaacagtggg
      181 aaccttgttc cagatgctgc ttccaaacat aaacaactgt cggagcttct acgaggaggc
      241 agcggctcta gtatcaaccc aggaatagga aatgtgagcg ccagcagccc cgtgcagcag
      301 ggcctgggtg gccaggctca agggcagccg aacagtgcta acatggccag cctcagtgcc
      361 atgggcaaga gccctctgag ccagggagat tcttcagccc ccagcctgcc taaacaggca
      421 gccagcacct ctgggcccac ccccgctgcc tcccaagcac tgaatccgca agcacaaaag
      481 caagtggggc tggcgactag cagccctgcc acgtcacaga ctggacctgg tatctgcatg
      541 aatgctaact ttaaccagac ccacccaggc ctcctcaata gtaactctgg ccatagctta
      601 attaatcagg cttcacaagg gcaggcgcaa gtcatgaatg gatctcttgg ggctgctggc
      661 agaggaaggg gagctggaat gccgtaccct actccagcca tgcagggcgc ctcgagcagc
      721 gtgctggctg agaccctaac gcaggtttcc ccgcaaatga ctggtcacgc gggactgaac
      781 accgcacagg caggaggcat ggccaagatg ggaataactg ggaacacaag tccatttgga
      841 cagcccttta gtcaagctgg agggcagcca atgggagcca ctggagtgaa cccccagtta
      901 gccagcaaac agagcatggt caacagtttg cccaccttcc ctacagatat caagaatact
      961 tcagtcacca acgtgccaaa tatgtctcag atgcaaacat cagtgggaat tgtacccaca
     1021 caagcaattg caacaggccc cactgcagat cctgaaaaac gcaaactgat acagcagcag
     1081 ctggttctac tgcttcatgc tcataagtgt cagagacgag agcaagcaaa cggagaggtt
     1141 cgggcctgct cgctcccgca ttgtcgaacc atgaaaaacg ttttgaatca catgacgcat
     1201 tgtcaggctg ggaaagcctg ccaagccatc ctggggtctc cagctagtgg aattcaaaac
     1261 acaattggtt ctgttggcac agggcaacag aatgccactt ctttaagtaa cccaaatccc
     1321 atagacccca gctccatgca gcgagcctat gctgctctcg gactccccta catgaaccag
     1381 ccccagacgc agctgcagcc tcaggttcct ggccagcaac cagcacagcc tcaaacccac
     1441 cagcagatga ggactctcaa ccccctggga aataatccaa tgaacattcc agcaggagga
     1501 ataacaacag atcagcagcc cccaaacttg atttcagaat cagctcttcc gacttccctg
     1561 ggggccacaa acccactgat gaacgatggc tccaactctg gtaacattgg aaccctcagc
     1621 actataccaa cagcagctcc tccttctagc accggtgtaa ggaaaggctg gcacgaacat
     1681 gtcactcagg acctgcggag ccatctagtg cataaactcg tccaagccat cttcccaaca
     1741 cctgatcccg cagctctaaa ggatcgccgc atggaaaacc tggtagccta tgctaagaaa
     1801 gtggaagggg acatgtacga gtctgccaac agcagggatg aatattatca cttattagca
     1861 gagaaaatct acaagataca aaaagaacta gaagaaaaac ggaggtcgcg tttacataaa
     1921 caaggcatct tggggaacca gccagcctta ccagccccgg gggctcagcc ccctgtgatt
     1981 ccacaggcac aacctgtgag acctccaaat ggacccctgt ccctgccagt gaatcgcatg
     2041 caagtttctc aagggatgaa ttcatttaac cccatgtcct tggggaacgt ccagttgcca
     2101 caagcaccca tgggacctcg tgcagcctcc ccaatgaacc actctgtcca gatgaacagc
     2161 atgggctcag tgccagggat ggccatttct ccttcccgaa tgcctcagcc tccgaacatg
     2221 atgggtgcac acaccaacaa catgatggcc caggcgcccg ctcagagcca gtttctgcca
     2281 cagaaccagt tcccgtcatc cagcggggcg atgagtgtgg gcatggggca gccgccagcc
     2341 caaacaggcg tgtcacaggg acaggtgcct ggtgctgctc ttcctaaccc tctcaacatg
     2401 ctggggcctc aggccagcca gctaccttgc cctccagtga cacagtcacc actgcaccca
     2461 acaccgcctc ctgcttccac ggctgctggc atgccatctc tccagcacac gacaccacct
     2521 gggatgactc ctccccagcc agcagctccc actcagccat caactcctgt gtcgtcttcc
     2581 gggcagactc ccaccccgac tcctggctca gtgcccagtg ctacccaaac ccagagcacc
     2641 cctacagtcc aggcagcagc ccaggcccag gtgaccccgc agcctcaaac cccagttcag
     2701 cccccgtctg tggctacccc tcagtcatcg cagcaacagc cgacgcctgt gcacgcccag
     2761 cctcctggca caccgctttc ccaggcagca gccagcattg ataacagagt ccctaccccc
     2821 tcctcggtgg ccagcgcaga aaccaattcc cagcagccag gacctgacgt acctgtgctg
     2881 gaaatgaaga cggagaccca agcagaggac actgagcccg atcctggtga atccaaaggg
     2941 gagcccaggt ctgagatgat ggaggaggat ttgcaaggag cttcccaagt taaagaagaa
     3001 acagacatag cagagcagaa atcagaacca atggaagtgg atgaaaagaa acctgaagtg
     3061 aaagtagaag ttaaagagga agaagagagt agcagtaacg gcacagcctc tcagtcaaca
     3121 tctccttcgc agccgcgcaa aaaaatcttt aaaccagagg agttacgcca ggccctcatg
     3181 ccaaccctag aagcactgta tcgacaggac ccagagtcat tacctttccg gcagcctgta
     3241 gatccccagc tcctcggaat tccagactat tttgacatcg taaagaatcc catggacctc
     3301 tccaccatca agcggaagct ggacacaggg caataccaag agccctggca gtacgtggac
     3361 gacgtctggc tcatgttcaa caatgcctgg ctctataatc gcaagacatc ccgagtctat
     3421 aagttttgca gtaagcttgc agaggtcttt gagcaggaaa ttgaccctgt catgcagtcc
     3481 cttggatatt gctgtggacg caagtatgag ttttccccac agactttgtg ctgctatggg
     3541 aagcagctgt gtaccattcc tcgcgatgct gcctactaca gctatcagaa taggtatcat
     3601 ttctgtgaga agtgtttcac agagatccag ggcgagaatg tgaccctggg tgacgaccct
     3661 tcacagcccc agacgacaat ttcaaaggat cagtttgaaa agaagaaaaa tgatacctta
     3721 gaccccgaac ctttcgttga ttgcaaggag tgtggccgga agatgcatca gatttgcgtt
     3781 ctgcactatg acatcatttg gccttcaggt tttgtgtgcg acaactgctt gaagaaaact
     3841 ggcagacctc gaaaagaaaa caaattcagt gctaagaggc tgcagaccac aagactggga
     3901 aaccacttgg aagaccgagt gaacaaattt ttgcggcgcc agaatcaccc tgaagccggg
     3961 gaggtttttg tccgagtggt ggccagctca gacaagacgg tggaggtcaa gcccgggatg
     4021 aagtcacggt ttgtggattc tggggaaatg tctgaatctt tcccatatcg aaccaaagct
     4081 ctgtttgctt ttgaggaaat tgacggcgtg gatgtctgct tttttggaat gcacgtccaa
     4141 gaatacggct ctgattgccc ccctccaaac acgaggcgtg tgtacatttc ttatctggat
     4201 agtattcatt tcttccggcc acgttgcctc cgcacagccg tttaccatga gatccttatt
     4261 ggatatttag agtatgtgaa gaaattaggg tatgtgacag ggcacatctg ggcctgtcct
     4321 ccaagtgaag gagatgatta catcttccat tgccacccac ctgatcaaaa aatacccaag
     4381 ccaaaacgac tgcaggagtg gtacaaaaag atgctggaca aggcgtttgc agagcggatc
     4441 atccatgact acaaggatat tttcaaacaa gcaactgaag acaggctcac cagtgccaag
     4501 gaactgccct attttgaagg tgatttctgg cccaatgtgt tagaagagag cattaaggaa
     4561 ctagaacaag aagaagagga gaggaaaaag gaagagagca ctgcagccag tgaaaccact
     4621 gagggcagtc agggcgacag caagaatgcc aagaagaaga acaacaagaa aaccaacaag
     4681 aacaaaagca gcatcagccg cgccaacaag aagaagccca gcatgcccaa cgtgtccaat
     4741 gacctgtccc agaagctgta tgccaccatg gagaagcaca aggaggtctt cttcgtgatc
     4801 cacctgcacg ctgggcctgt catcaacacc ctgcccccca tcgtcgaccc cgaccccctg
     4861 ctcagctgtg acctcatgga tgggcgcgac gccttcctca ccctcgccag agacaagcac
     4921 tgggagttct cctccttgcg ccgctccaag tggtccacgc tctgcatgct ggtggagctg
     4981 cacacccagg gccaggaccg ctttgtctac acctgcaacg agtgcaagca ccacgtggag
     5041 acgcgctggc actgcactgt gtgcgaggac tacgacctct gcatcaactg ctataacacg
     5101 aagagccatg cccataagat ggtgaagtgg gggctgggcc tggatgacga gggcagcagc
     5161 cagggcgagc cacagtcaaa gagcccccag gagtcacgcc ggctgagcat ccagcgctgc
     5221 atccagtcgc tggtgcacgc gtgccagtgc cgcaacgcca actgctcgct gccatcctgc
     5281 cagaagatga agcgggtggt gcagcacacc aagggctgca aacgcaagac caacgggggc
     5341 tgcccggtgt gcaagcagct catcgccctc tgctgctacc acgccaagca ctgccaagaa
     5401 aacaaatgcc ccgtgccctt ctgcctcaac atcaaacaca agctccgcca gcagcagatc
     5461 cagcaccgcc tgcagcaggc ccagctcatg cgccggcgga tggccaccat gaacacccgc
     5521 aacgtgcctc agcagagtct gccttctcct acctcagcac cgcccgggac ccccacacag
     5581 cagcccagca caccccagac gccgcagccc cctgcccagc cccaaccctc acccgtgagc
     5641 atgtcaccag ctggcttccc cagcgtggcc cggactcagc cccccaccac ggtgtccaca
     5701 gggaagccta ccagccaggt gccggccccc ccacccccgg cccagccccc tcctgcagcg
     5761 gtggaagcgg ctcggcagat cgagcgtgag gcccagcagc agcagcacct gtaccgggtg
     5821 aacatcaaca acagcatgcc cccaggacgc acgggcatgg ggaccccggg gagccagatg
     5881 gcccccgtga gcctgaatgt gccccgaccc aaccaggtga gcgggcccgt catgcccagc
     5941 atgcctcccg ggcagtggca gcaggcgccc cttccccagc agcagcccat gccaggcttg
     6001 cccaggcctg tgatatccat gcaggcccag gcggccgtgg ctgggccccg gatgcccagc
     6061 gtgcagccac ccaggagcat ctcacccagc gctctgcaag acctgctgcg gaccctgaag
     6121 tcgcccagct cccctcagca gcaacagcag gtgctgaaca ttctcaaatc aaacccgcag
     6181 ctaatggcag ctttcatcaa acagcgcaca gccaagtacg tggccaatca gcccggcatg
     6241 cagccccagc ctggcctcca gtcccagccc ggcatgcaac cccagcctgg catgcaccag
     6301 cagcccagcc tgcagaacct gaatgccatg caggctggcg tgccgcggcc cggtgtgcct
     6361 ccacagcagc aggcgatggg aggcctgaac ccccagggcc aggccttgaa catcatgaac
     6421 ccaggacaca accccaacat ggcgagtatg aatccacagt accgagaaat gttacggagg
     6481 cagctgctgc agcagcagca gcaacagcag cagcaacaac agcagcaaca gcagcagcag
     6541 caagggagtg ccggcatggc tgggggcatg gcggggcacg gccagttcca gcagcctcaa
     6601 ggacccggag gctacccacc ggccatgcag cagcagcagc gcatgcagca gcatctcccc
     6661 ctccagggca gctccatggg ccagatggcg gctcagatgg gacagcttgg ccagatgggg
     6721 cagccggggc tgggggcaga cagcaccccc aacatccagc aagccctgca gcagcggatt
     6781 ctgcagcaac agcagatgaa gcagcagatt gggtccccag gccagccgaa ccccatgagc
     6841 ccccagcaac acatgctctc aggacagcca caggcctcgc atctccctgg ccagcagatc
     6901 gccacgtccc ttagtaacca ggtgcggtct ccagcccctg tccagtctcc acggccccag
     6961 tcccagcctc cacattccag cccgtcacca cggatacagc cccagccttc gccacaccac
     7021 gtctcacccc agactggttc cccccacccc ggactcgcag tcaccatggc cagctccata
     7081 gatcagggac acttggggaa ccccgaacag agtgcaatgc tcccccagct gaacaccccc
     7141 agcaggagtg cgctgtccag cgaactgtcc ctggtcgggg acaccacggg ggacacgcta
     7201 gagaagtttg tggagggctt gtagtaatga actagtccgt aacttgaaag tatttcgatt
     7261 tcttggcttt atatatcttg tggaaaggac gaactagacg aggcacagat aggtcg
//
