LOCUS       sequence-282546-        2051 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2051)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2051)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1135
FEATURES             Location/Qualifiers
     source          1..2051
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggaacaatc taatgattca ttaagagtca accataatga cggtgaagag
       61 tcaaaaacca gtgctcaagt atttgagatg atcgttgcca gcccaacaga aaatggacag
      121 gtacttcgtg taattccacc tacccagaca ggaatggcac aagtgattat acctcagggg
      181 caacttgtgg atgtgaatag tcctcgggat gtccctgaag agaaacccag taacagaaac
      241 ttaccaactg taagagtgga tactctagca gacaatacca gcaattacat tcttcatcct
      301 caaacatcct tcccattgcc caaaaagtca gtgaccggaa tgctggaaga accccttctg
      361 gggcctcttc agccactttc ttctaataca cctatatggg cctgccgtct taggagctgt
      421 gagaaaattg gagattcata ccgtggctac tgtgtaagtg agactgaatt agaaagtgtc
      481 ctaacatttc acaagcagca aacacagagt gtttggggga cccgtcagtc tccaagccca
      541 gccaagcctg ctacacgctt gatgtggaaa tcccagtatg ttccatatga tggaatccca
      601 tttgttaatg cagggagtag agctgtggta atggagtgtc agtatgggcc aagaagaaaa
      661 ggtttccagt taaaaaaagt cagtgagcag gaaagcaggt cttgtcagct ctacaaagcc
      721 acttgtccag ctcggattta cattaaaaag gtacagaagt ttcctgaata tagagttcct
      781 acagacccca aaattgacaa gaaaattatc agaatggagc aggagaaagc ttttaacatg
      841 ctaaagaaga acttggtaga tgctggtggt gttcttaggt ggtatgtaca gttacctaca
      901 cagcaagctc atcagtatca tgaattagag actccctgcc tcactttgtc accttctcct
      961 tttcctgtgt cttctcttga agaagaggaa actgcagtta gagatgagaa ttgtgcatta
     1021 ccctcacgtt tacatcctca agtagcacat aagattcaag aattagtatc acagggaata
     1081 gaacaagtgt atgcagtaag gaaacagcta agaaaatttg tggaaaggga actgttcaaa
     1141 cccgatgagg tacctgaaag acataattta tctttttttc caactgtaaa tgatataaaa
     1201 aatcacatcc atgaggtaca gaaatccttg agaaatggag atacggtata taactcagag
     1261 attattccag caacgcttca atggactaca gacagtggga atattctcaa agagaccatg
     1321 acagttacat ttgcagaagg aaattcacca ggagaatcaa ttaccaccaa agtggaaaca
     1381 aatcagacca ggggttcttt gtctcctgag ccaacccact tgctctcctc actctcctca
     1441 tttcagccca aaatatttac acaactacag ggtttgcagt tacaaccaag gtacacctct
     1501 cctgatgaat caccagctgt ggtatcagta aataaccagc cgtcctctag tccttcagga
     1561 cttctggata caataggaag tgctgtaatg aataataatt ctctactgct tggtcaaagt
     1621 catagccttc aaagagatac atgcttaacc caaaacaata gtactgcctc caccatgggt
     1681 aaccttccag aaccagatca aaatctagtt gcaatggacg agctggtaga agttggagat
     1741 gttgaggata cagggaatct ggaaggaact gttcatcgga ttctgttggg agatgtgcag
     1801 actattccaa tacagattat agacaaccac tcagctctta ttgaagaaaa tccagaaagt
     1861 accatttctg tgagccaagt taaacaagaa cccaaagaac cagcattgtc tatggaagca
     1921 aaaaaaactg tggactataa gaaattatct gctacatagt aatgaactag tccgtaactt
     1981 gaaagtattt cgatttcttg gctttatata tcttgtggaa aggacgattc gaagtggcag
     2041 ctattatggt t
//
