LOCUS       sequence-282552-        1898 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1898)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1898)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1166
FEATURES             Location/Qualifiers
     source          1..1898
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggacgctct gccccgcagc gggctgaacc tgaaggagga gccgctgctg
       61 cccgccggcc tgggctcagt gcgctcctgg atgcagggcg cgggcatcct ggacgccagc
      121 accgcggcgc agagtggcgt gggtctggca cgagcacatt ttgagaagca gcctccctcc
      181 aacctcagga aatccaactt cttccacttc gtgctggcca tgtacgaccg gcaggggcag
      241 cccgtggagg tggagcgcac agccttcatc gacttcgtgg aaaaggaccg agagcccggg
      301 gcggaaaaga ctaacaatgg gatccattac cgcctccggc tggtgtataa caatggactg
      361 cggacagagc aagacctcta cgtgcgtctc atcgactcca tgtccaaaca ggccatcatc
      421 tatgaggggc aggacaagaa ccccgaaatg tgccgagtgc tgctcaccca tgagatcatg
      481 tgcagccggt gctgtgaccg gaagagctgt ggcaaccgga atgagacgcc ctcagacccc
      541 gtcatcattg acaggttctt cctcaagttc ttcctcaaat gcaaccagaa ctgcctgaag
      601 aatgcgggga atcccagaga catgcgccgc ttccaggtgg tggtgtccac gacggtgagc
      661 gtggacggac acgtgctggc cgtgtccgac aacatgtttg tgcacaacaa ctccaagcat
      721 ggccgcaggg cgcgccgcct ggacccctcc gaagctgcca ccccctgcat caaggccatc
      781 agccccgggg agggctggac cacgggcggc gccaccgtca ttgtcatcgg cgacaacttc
      841 ttcgacgggt tgcaggtcgt gttcggaaac gtgctcgtgt ggagcgagct catcacgccc
      901 cacgccatcc gggtgcagac gcccccgcgg cacatccccg gggtggtgga ggtgaccctc
      961 tcctacaagt ccaagcagtt ttgcaaggga tgccccggcc gctttgtcta cacagctctg
     1021 aacgagccca ccattgacta cggattccag aggctacaga aagtcattcc cagacacccc
     1081 ggagaccccg agaggctgcc caaggaagtg ctgctgaagc gggcggccga cttggcagaa
     1141 gccctgtacg gagtgcccgg cagtaaccag gagctgctcc tgaagcgcgc ggcggacgtg
     1201 gccgaggctc tgtacagcac cccccgcgca cccgggccgc tcgcacccct ggccccgagc
     1261 cacccacacc ccgccgtcgt gggcatcaac gccttcagca gcccgctggc catcgccgtc
     1321 ggggacgcca ccccggggcc cgagccgggc tacgcgcgca gctgcagcag cgcgtccccc
     1381 cgcgggttcg cgcccagccc cggctcgcag cagagcggct acggcggcgg cctcggagct
     1441 ggcctgggcg gctacggcgc gccgggcgtg gccggcctcg gcgtgcctgg gtcccccagc
     1501 ttcctcaatg gctccaccgc cacctcgccc ttcgccatca tgccctctag ccccccgctg
     1561 gcggctgcct cctccatgtc cctcccggcc gctgccccca ccaccagcgt gttctccttc
     1621 tcgcctgtca acatgatctc cgccgtcaaa cagaggagcg ccttcgcccc cgtgctgcgc
     1681 cccccaagct ccccacccca ggcctgcccc agagcccacg gagaggggct tccagaccag
     1741 tcttttgagg attctgacaa gtttcactct ccagcccggg ggcttcaggg cctggcatac
     1801 tcctagtaat gaactagtcc gtaacttgaa agtatttcga tttcttggct ttatatatct
     1861 tgtggaaagg acgatcgggt ctgctcaata ctaacacg
//
