LOCUS       sequence-282582-        1382 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1382)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1382)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1197
FEATURES             Location/Qualifiers
     source          1..1382
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             1018..1026
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 accgccacca tgaaaaatgc cacgattgta atgtctgtga ggagagagca aggcagttct
       61 tcaggggagg ggtcattgtc attcgaagat gtggctgtgg gcttcaccag ggaggagtgg
      121 cagtttttgg accagtctca gaaggtcttg tacaaggaag taatgttgga gaactacatc
      181 aacctagtat caatagggta tcgaggcacc aagccagatt cgctcttcaa gttggagcaa
      241 ggagaacccc cagggatagc agaaggagca gcccacagtc aaatctgtcc aggagggaaa
      301 ccacatgaat gcagtgtgtg cgggagagcc ttctccagga aagcacagct tattcaacat
      361 cagagaactg aaagaggaga gaaaccccat ggatgtggtg aatgtgggaa aacattcatg
      421 aggaagattc agctcactga gcatcagaga actcacacag gagagaagcc ccatgaatgt
      481 agtgaatgtg gaaaagcctt ctccagaaag tcacagctca tggtccatca gagaacccat
      541 acaggagaga aaccctacag atgcagcaaa tgcggaaaag ccttcagccg gaagtgccgg
      601 ctcaatagac atcagcgatc acatactgga gagaaactct atgggtgcag tgtgtgtggg
      661 aaagcctttt ctcagaaggc atacctcact gcacaccaga gacttcacac aggagataag
      721 ccttataaat gcagtgattg tggaagaacc ttctatttta agtcagacct gaccagacat
      781 cagaggattc atacgggaga gaaaccttat gaatgtagtg agtgtgaaaa agcctttaga
      841 agcaagtcga agctcattca gcatcagcgt actcacactg gagagagacc atattcatgc
      901 agagaatgtg gcaaagcctt tgcccacatg tcagtcctca ttaaacatga gaaaactcac
      961 ataagagaga cagccataaa ttcactgacg gtggagaaac cttcctcaag gagtcatacc
     1021 tccttataca tgagcgaact catacaagag caaaagactg tgaacacagt acctatagaa
     1081 atgccttcct caggaacccc gccattgtta aacaagagtg agcgcctagt gggcagaaat
     1141 gtagtgattg tggaacaacc ttttccaaga aatcaagcct ttgtagttaa tcaggaattt
     1201 gaacagagaa taagcctcac aaatgaagtg aatgtggccc catcagtaat aaattatatc
     1261 ttgtatctta cagatattgt atcagaatag taatgaacta gtccgtaact tgaaagtatt
     1321 tcgatttctt ggctttatat atcttgtgga aaggacgatt tgaaatcgta tttattgtgg
     1381 ga
//
