LOCUS       sequence-282585-        7442 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 7442)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 7442)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1269
FEATURES             Location/Qualifiers
     source          1..7442
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggacactgg ggacacagct ctaggacaaa aagctacctc aaggtctgga
       61 gaaactgata aagcatcagg tagatggaga caggaacaat cagctgttat taagatgagc
      121 acttttggca gtcatgaagg acagcggcaa ccacaaatag agcctgagca aatcggaaac
      181 acagcatcag cacaactgtt tggttctggg aaactggcct cccctagtga agtggtgcag
      241 caagtcgcag agaagcaata tccaccgcat cgtccgagtc cttactcatg ccaacactca
      301 ctctctttcc ctcagcactc attgccacag ggggtcatgc acagcaccaa gccacatcag
      361 agcctcgaag gtcctccgtg gcttttccct ggccctttgc catccgttgc ctctgaggac
      421 ttatttcctt ttcctataca tggccacagt ggtggttatc ctagaaaaaa gatttcaagt
      481 ctgaaccctg cttatagcca atactcccag aaaagtattg aacaggcaga agaggctcac
      541 aagaaagagc acaaacccaa aaagcctggc aagtacattt gcccttactg cagcagagcg
      601 tgtgccaaac ctagtgtact gaaaaaacac atcaggtccc atactgggga gcggccatat
      661 ccatgtatac cttgtggttt ctctttcaag acaaagagca atttgtacaa gcacaggaag
      721 tcacatgccc atgcaattaa ggcaggatta gtacctttca cagagtcagc tgtatctaaa
      781 ttggacctag aggctggttt tattgatgta gaagcagaaa tacattcaga tggtgaacag
      841 agtacagaca cagatgagga gagttcttta tttgccgagg cttctgacaa aatgagtcct
      901 ggtccaccca tcccactgga cattgccagc agaggcggct atcatgggtc attggaagaa
      961 tcattgggag gtccaatgaa ggtgccgatt ttgattatcc ctaaaagtgg gattcctctc
     1021 cctaatgaaa gctctcagta tattggccct gatatgctac caaatccatc tttaaatact
     1081 aaggctgatg attcgcacac agtcaaacag aaacttgcac taagactgtc agagaaaaaa
     1141 ggacaagatt ctgagccatc gctcaacctt ctgagcccgc acagtaaagg aagcactgat
     1201 tctggttact tttctcgctc agaaagtgct gagcagcaaa taagccctcc caacacaaat
     1261 gcaaagtctt atgaagaaat catctttgga aaatactgtc ggcttagtcc gagaaatgca
     1321 ctcagtgtta caaccacaag tcaggagcgt gccgcaatgg gtaggaaggg cataatggaa
     1381 ccattacctc acgttaacac caggttagat gtcaagatgt ttgaagatcc tgtttcacag
     1441 ctgatcccaa gcaagggaga tgtcgacccc agtcaaacga gcatgctgaa atccactaag
     1501 ttcaacagtg agtccagaca accccagatt attccatcat ctatcaggaa cgaaggaaaa
     1561 ctttatccag caaacttcca aggcagcaac ccggttctct tagaagctcc tgtagactct
     1621 tcacccctta ttagaagcaa ctcagtgcca acttcttcag caactaatct aactattcct
     1681 ccttctttga gaggaagtca ctcatttgat gaaaggatga ctggttccga cgatgtattc
     1741 tatccaggga ccgtgggcat accccctcag cgcatgctaa gaagacaagc ggcatttgag
     1801 ctgccttcgg tacaggaggg ccacgtggaa gtcgagcacc atggcaggat gttgaagggt
     1861 atcagcagtt catccctgaa ggaaaagaaa ttgtctcctg gggacagggt tgggtatgac
     1921 tatgatgtct gtcggaaacc ctataagaag tgggaggact ctgaaacacc aaagcaaaac
     1981 tacagggaca tttcctgctt gagttcttta aagcatggtg gagaatattt catggatccc
     2041 gtggtgccat tgcagggagt accaagcatg tttggaacta cctgtgaaaa caggaaacgc
     2101 cggaaagaga agagcgtagg ggatgaagag gacacgccca tgatctgcag cagcattgta
     2161 agcactcctg tgggcatcat ggcttccgat tatgacccca aactgcagat gcaggaagga
     2221 gtcaggagtg gatttgccat ggctggacac gaaaaccttt ctcatggtca cacggaacgc
     2281 tttgacccat gtcggcccca actgcagcct ggaagtccat ctcttgtgtc agaggagtca
     2341 ccttcagcca ttgattcaga caagatgtca gacctagggg gcaggaaacc tcctggaaat
     2401 gtgatttctg tgattcagca caccaactca ctgagccgac ccaattcatt tgaaaggtct
     2461 gagtcagccg aacttgtggc ttgcacacag gataaagccc cttccccttc agagacttgt
     2521 gacagtgaga tttcagaagc cccagtgagt cctgagtggg ctccacctgg ggatggtgca
     2581 gaaagtgggg ggaaaccctc tccatctcag caggtgcagc agcagtccta tcacacacag
     2641 cccaggctag ttcggcaaca caacatccag gttcctgaga ttcgagtgac cgaggagcct
     2701 gataaacctg agaaggagaa ggaagcccag agcaaagagc cagagaagcc tgtggaagaa
     2761 tttcagtggc cccagagaag tgagaccctt tcccagctcc ccgcggagaa gttgccaccc
     2821 aaaaagaagc gtctgcgact tgcagatatg gagcactcct caggggagtc cagctttgaa
     2881 tccacaggca caggcctctc ccgcagcccc agccaagaaa gcaacttgtc ccacagctcc
     2941 agtttctcca tgtcttttga aagagaagaa accagtaagc tttctgcact tcctaagcag
     3001 gatgagtttg ggaagcattc agagtttctg actgtccctg ctggttcata ctcattgtct
     3061 gtcccaggcc atcaccacca gaaagagatg cgacgctgct catcagagca gatgccttgt
     3121 cctcacccag cggaagtccc agaagttcgg agcaaatcat ttgattatgg gaatctgtcc
     3181 catgctcctg tgtcgggagc agcagcctcc acggtatcac cgtccaggga gaggaagaaa
     3241 tgctttctgg tgcggcaagc ttccttcagt ggctccccag aaatctccca gggcgaggtt
     3301 ggcatggatc agagcgtgaa gcaagagcag ctggagcacc tgcatgctgg cctccggtcc
     3361 gggtggcacc atggcccgcc tgctgtgctg cctcctcttc agcaagagga cccagggaag
     3421 caggtggcgg gtccttgtcc cccgctgagc tcggggccac tgcacctggc ccagccacag
     3481 atcatgcaca tggacagtca ggaatctttg agaaatccct tgatccaacc aacatcctat
     3541 atgacaagca agcacttacc tgaacagcca cacttatttc cacatcaaga gacaattcca
     3601 ttttctccaa tccagaatgc cttgtttcag tttcagtatc ctacagtttg tatggttcat
     3661 ttaccagctc agcagcctcc ctggtggcag gcacatttcc cacatccctt tgctcagcac
     3721 cctcagaaga gctatggcaa gccctctttt cagacagaaa tccattcgag ctatccctta
     3781 gagcatgtgg cagagcacac tggaaagaaa cctgctgagt atgcacacac gaaagagcag
     3841 acctacccat gttattcagg agcatcaggg ctacacccaa agaaccttct tccaaagttt
     3901 ccatcagacc agagcagtaa gtcaactgaa acgccctctg agcaggttct tcaagaagat
     3961 tttgcctcgg caaatgctgg gtctttgcag tccctcccag gaacagtggt tcctgttcgg
     4021 atccagacgc acgtaccatc ctatggaagt gtcatgtaca caagcatttc tcagatactt
     4081 gggcagaata gccctgccat tgtcatatgc aaagtcgatg agaatatgac ccaaaggaca
     4141 ctggtcacca acgcagccat gcaagggata ggattcaaca ttgcccaggt gctggggcag
     4201 catgcgggct tggagaagta ccccatttgg aaagcacctc agactttgcc cctcggctta
     4261 gaatcctcca tccccttgtg tttaccttcc acctctgaca gcgtggccac cctgggaggt
     4321 agcaagcgaa tgctttctcc agccagtagc ttggagctct tcatggaaac caagcagcag
     4381 aaaagggtca aagaagaaaa gatgtacgga cagattgtgg aggagcttag tgctgtggag
     4441 ctgaccaact cagacatcaa aaaggacctc tcccgccccc agaaacccca gctggttcga
     4501 caaggatgtg cttctgagcc aaaagatggc ttgcagtcag ggtcatcttc cttctcctcg
     4561 ctgtcgccct cctcatctca agactatcct tctgttagcc cgtcttccag ggagccattc
     4621 ctgcccagca aggagatgct ttccggttcc cgggcaccac ttccggggca gaagtccagt
     4681 gggccttctg aaagcaaaga atcttcagat gaattagata tcgatgagac ggcatcggac
     4741 atgagcatga gcccacagag ttcttcatta ccagcaggag atggtcagct ggaagaggaa
     4801 gggaagggcc acaagcggcc tgttggcatg ctggtccgca tggcctctgc ccccagcggg
     4861 aacgtggcag actcaactct tcttctcacg gacatggcag atttccagca gattcttcag
     4921 ttccccagtc tgcggacaac aactactgtg agttggtgct tcttgaatta tacaaaaccc
     4981 aattatgtgc aacaggccac cttcaaatcc tcggtttatg cttcatggtg cattagttcc
     5041 tgtaatccaa acccatcagg attgaacacc aagaccacgc tggctcttct gaggtccaag
     5101 caaaaaatca ctgcagaaat ttatactctg gctgctatgc ataggcctgg aaccggcaag
     5161 cttacatcat caagtgcttg gaagcagttt actcagatga aacctgatgc gtccttttta
     5221 tttggcagca aactagaaag gaaactagtg ggaaatatct taaaggaaag agggaaagga
     5281 gatattcatg gagataaaga tattggatcc aaacaaactg agccaatccg aattaaaata
     5341 tttgaaggag ggtacaaatc gaatgaagat tatgtatatg tcagaggacg tggccgggga
     5401 aagtacattt gtgaagaatg tgggattcgc tgtaagaagc caagcatgct caaaaaacac
     5461 atccgtaccc atactgatgt tcggccttat gtatgcaagt tatgtaactt tgccttcaaa
     5521 acgaaaggaa acctaacgaa gcatatgaaa tctaaagcac acatgaaaaa atgcctggaa
     5581 ttgggagtct caatgacatc ggtggatgat acagaaactg aggaagcaga aaatttggaa
     5641 gatttgcaca aagcagcaga gaagcatagc atgtccagca tttcaactga tcatcagttc
     5701 tccgatgctg aggaatcaga tggtgaggat ggagatgata atgatgatga tgatgaagat
     5761 gaagatgact ttgacgacca gggagattta acaccaaaaa caagatcaag aagcaccagt
     5821 cctcagcctc ctagattctc ctccttgcct gtgaatgttg gcgccgtacc ccacggggtt
     5881 ccttcagata gttccctggg acattcttcg ttgatcagct atttggttac tttgccaagt
     5941 attcgagtta ctcagcttat gacacccagt gattcatgtg aagataccca gatgacagaa
     6001 taccagaggc tattccagag caaaagtacg gactcagaac cagacaaaga cagattggac
     6061 atacctagtt gtatggatga ggagtgcatg ctaccttcag agccaagctc ctctcccagg
     6121 gacttctcac cctcaagcca ccattcctct ccaggatatg attcttcacc ctgtcgagat
     6181 aattcaccaa agaggtatct gatacccaaa ggagatttat ctcccaggag acatttatca
     6241 cctaggagag atctgtcacc catgagacat ctttcaccaa gaaaggaagc tgcattgaga
     6301 agagagatgt cccaaagaga tgtttcacca agaaggcatt tgtctccaag gaggccagtg
     6361 tctcctggga aagatatcac agcaagaaga gacctctctc ctagaagaga gagaagatac
     6421 atgaccacaa taagagcgcc atctcccaga agggctttat accataaccc accattgtcc
     6481 atgggacagt atttgcaagc agagccaatt gtattggggc ctcctaattt aagaagagga
     6541 ttacctcagg ttccttactt cagtctctat ggagaccaag aaggtgctta tgaacatcca
     6601 ggctccagcc ttttccctga gggtcctaat gactatgtct tcagtcatct tccactccac
     6661 tctcagcaac aagtgcgagc ccctatcccc atggtgcccg ttggtgggat ccagatggtt
     6721 cactccatgc cgccagccct ttccagttta catccttcac ccacattgcc cctgccaatg
     6781 gagggctttg aggagaagaa aggcgcgtca ggggagtcct tctccaagga cccctatgtg
     6841 ctttctaagc agcatgagaa gcgaggtcct cacgctttgc agtcatctgg tccacctagc
     6901 actccctcct ctcctcggct gttgatgaaa cagagcactt cggaagacag cctaaacgca
     6961 acagagcggg aacaggagga aaatatacag acttgtacaa aagccattgc ctctctccgg
     7021 attgccacgg aagaggcagc tctgctcggg ccagatcagc cagcgcgggt gcaggagccc
     7081 caccagaacc ccctgggaag tgcacatgtt agcattagac actttagtag acctgagcca
     7141 ggtcagccct gtacctcagc cacccaccct gacttgcatg atggtgaaaa ggacaatttt
     7201 ggtacatcac agactccatt agctcactcc acgttttaca gcaagagttg tgtggatgac
     7261 aagcagttgg actttcacag cagcaaggaa ttatcttcaa gcacagagga aagcaaagat
     7321 ccttcatcag aaaagagtca gctacattag taatgaacta gtccgtaact tgaaagtatt
     7381 tcgatttctt ggctttatat atcttgtgga aaggacgaga gatgctgctc agagttgctc
     7441 cg
//
