LOCUS       sequence-282618-        2474 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2474)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2474)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1415
FEATURES             Location/Qualifiers
     source          1..2474
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcttactg cctgacaaat tgttatcagt actccgtgac ctttgaggat
       61 acagctgtgg acttcaccca ggaggagtgg attttactgg acccagttca gagaaactta
      121 tacagagatg tgatgctgga gaattatgag aacgtggcca aagtaggatt tcagctcttt
      181 aaacccagtg tcatctcttg gttggaagaa gaagagttga gaaccttgca gcaaggagtt
      241 ctccaagact gggcaataaa acatcaaacc agtgtttcag cactgcagca ggagttttgg
      301 aaaatacaaa cttctaatgg gatacaaatg gacctggtaa cctttgacag tgtggctgtg
      361 gagttcaccc aggaagagtg gactttactg gacccagctc agagaaacct gtacagtgat
      421 gtgatgctgg agaactacaa gaacctgtcc tcagtaggtt accagctctt caaacccagt
      481 ctgatctctt ggctggagga agaggaagag ttgagcacac tgccaagagt tctccaagaa
      541 tggaaaatgt gcctgaaaac caaagggcca gccctttggc aagataattt ttgtttaaaa
      601 acattaaatg ggatacagtt ggcaagaaac caaaatggag aggaactcta tgactgtaag
      661 caatgtgaag atgtcttctg taaacatcca tgccttaaga ccaacatgag tactcaaaat
      721 agaggcaaca cgtctgaatg tattcagtat gcaaaagacc tcctttctct atacaataaa
      781 acctctacca taaggaaagt ttctgtgttc agtaagcatg gaaaatcttt ccgcctgatt
      841 ttaaatgttc aggtccagag aaagtgtaca caagataaat cctttgaagg cactgactat
      901 gggaaagcct tcatttatca gtcatacctt gaagcacaca ggaaaactca gagtggagaa
      961 aaactcaatg aatggaagca atgtggggaa gcatttactc actccacaag ccatgctgta
     1021 aatgttgaaa cacacattat taaaaacccc tatgaatgta aggaatgtgg aaaagatttt
     1081 agatacccta cccaccttaa taatcacatg caaacccaca ttgggataaa accttataaa
     1141 tgtaagcact gtggcaaaac cttcactgtg ccgtcaggct ttcttgaaca tgtacgaact
     1201 cacactggag agaagcccta tgggtgtaag gaatgtggta aagcctttgg tacatctgca
     1261 ggccttattg aacatataag atgtcacgcc agagagaaaa cctttaaatg tgaccactgt
     1321 gggaaagcct ttatttctta cccatctctt tttggacatt tgagagttca taatggagag
     1381 aagccatatg agcataagga atatgggaag gcctttggta catcctcagg tgttattgaa
     1441 gatagaagaa gtaacacagg acagaaacgc tttgattgtg accagtgtgg gaaagtcttt
     1501 gtttctttct catctctttt tgctcatttg agaactcaca ctggtgagaa gccctttaag
     1561 tgttacaaat gtgggaaacc atttacctct tctgcctgtc ttcgtattca catgcgaact
     1621 cacacagaag agagactcta tcaatgtaag aaatgtggta aagctttcac taagtgctca
     1681 tatcttacca aacatttacg aacacacgct ggagagaaac cctatgaatg tatgaaatgt
     1741 gggaaagcct tcactgagcg ctcatacctt actaaacatt tacgaagaca cagtggagag
     1801 aagccatatg aatgtaagaa atgtggaaaa gccttcacag aacgctcaga tcttactaaa
     1861 catttacgaa gacacactgg agataagccc tatgaatata aggactgtgg gaaagccttt
     1921 gttgtctcct ccagtctagt tgatcattta agaactcaca ctggatataa accctataaa
     1981 tgtaatgcat gtgaaaaagc ttacagtagg tcttgtgtac taactcaaca cttaaaaact
     2041 catgcagcag agaagacctc tgaatgtaac gcatgtggaa attcctttcg aaattccatg
     2101 tgctttcatg atcgcttaaa aactctcacc aaaataaaac cctataaatg taaggactgt
     2161 gggaaagcct tcacttgtca ttcagatctt actaatcatg tgcgaattca cactggagag
     2221 aagccctata aatgtaagga atgtgggaag gccttccgta catcctcagg acgtattcaa
     2281 catttaagaa ctcatatggg agagaaaccc tttgaatgtg accagtgtgg gaaagccttt
     2341 gcttctttct cagctcgtat tgcacatttg aaaacacact agtaatgaac tagtccgtaa
     2401 cttgaaagta tttcgatttc ttggctttat atatcttgtg gaaaggacga taacacagcc
     2461 aagcacggtg gtga
//
