LOCUS       sequence-28263-e         826 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 826)
  AUTHORS   Ebert MS, Neilson JR, Sharp PA
  TITLE     MicroRNA sponges: competitive inhibitors of small RNAs in mammalian 
            cells.
  JOURNAL   Nat Methods. 2007 Sep . 4(9):721-6.
  PUBMED    17694064
REFERENCE   2  (bases 1 to 826)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2007 Sep . 4(9):721-6."
COMMENT     Sequence Label: CMV-d2eGFP-cxcr4
FEATURES             Location/Qualifiers
     source          1..826
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     132..153
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(541..558)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    547..771
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg
       61 ctgctgcccg acaaccacta cctgagcacc cagtccgccc tgagcaaaga ccccaacgag
      121 aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg
      181 gacgagctgt acaagaagct tagccatggc ttcccgccgg aggtggagga gcaggatgat
      241 ggcacgctgc ccatgtcttg tgcccaggag agcgggatgg accgtcaccc tgcagcctgt
      301 gcttctgcta ggatcaatgt gtaggcggcc gcctcgagcc ggaagttttc agaaagctaa
      361 caccggaagt tttcagaaag ctaacaccgg aagttttcag aaagctaaca ccggaagttt
      421 tcagaaagct aacaccggaa gttttcagaa agctaacacc ggaagttttc agaaagctaa
      481 caccggaagt tttcagaaag ctaacaccgg atcgcgggcc cgtttaaacc cgctgatcag
      541 cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct
      601 tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc
      661 attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg
      721 aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg gcttctgang
      781 cggaaagaac cagctggggc tctagggggt atccccacgc gccctg
//
