LOCUS       sequence-28266-e        1028 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1028)
  AUTHORS   Ebert MS, Neilson JR, Sharp PA
  TITLE     MicroRNA sponges: competitive inhibitors of small RNAs in mammalian 
            cells.
  JOURNAL   Nat Methods. 2007 Sep . 4(9):721-6.
  PUBMED    17694064
REFERENCE   2  (bases 1 to 1028)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2007 Sep . 4(9):721-6."
COMMENT     Sequence Label: CMV-d2eGFP-16pf
FEATURES             Location/Qualifiers
     source          1..1028
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     161..182
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(437..454)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    443..667
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(800..819)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 gacttcagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc
       61 agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc
      121 agtccgccct gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg
      181 tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagaagctt agccatggct
      241 tcccgccgga ggtggaggag caggatgatg gcacgctgcc catgtcttgt gcccaggaga
      301 gcgggatgga ccgtcaccct gcagcctgtg cttctgctag gatcaatgtg taggcggccg
      361 cctcgagcgc caatatttac gtgctgctac gccaatattt acgtgctgct agggcccgtt
      421 taaacccgct gatcagcctc gactgtgcct tctagttgcc agccatctgt tgtttgcccc
      481 tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat
      541 gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg
      601 caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga tgcggtgggc
      661 tctatggctt ctgaggcgga aagaaccagc tggggctcta gggggtatcc ccacgcgccc
      721 tgtagcggcg cattaancgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
      781 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
      841 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
      901 cggcacctcg accccaaaaa cttgattagg gtgatggnnc gngtactaga agtcctattc
      961 cgagttctat ctctagaant ataggacttc ctggnaaaaa gcctgaactc ncgcgacgtc
     1021 tgtcanna
//
