LOCUS       sequence-282660-         977 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 977)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 977)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1506
FEATURES             Location/Qualifiers
     source          1..977
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcaagaaa gaaaaggaga gggattatag agaaaaggcg tcgggatcgg
       61 ataaataaca gtttatctga gttgagaaga cttgtgccaa ctgcttttga aaaacaagga
      121 tctgcaaagt tagaaaaagc tgaaatattg caaatgacag tggatcattt gaagatgctt
      181 caggcaacag ggggtaaagg ctactttgac gcacacgctc ttgccatgga cttcatgagc
      241 ataggattcc gagagtgcct aacagaagtt gcgcggtacc tgagctccgt ggaaggcctg
      301 gactcctcgg atccgctgcg ggtgcggctt gtgtctcatc tcagcacttg cgccacccag
      361 cgggaggcgg cggccatgac atcctccatg gcccaccacc atcatccgct ccacccgcat
      421 cactgggccg ccgccttcca ccacctgccc gcagccctgc tccagcccaa cggcctccat
      481 gcctcagagt caaccccttg tcgcctctcc acaacttcag aagtgcctcc tgcccacggc
      541 tctgctctcc tcacggccac gtttgcccat gcggattcag ccctccgaat gccatccacg
      601 ggcagcgtcg ccccctgcgt gccacctctc tccacctctc tcttgtccct ctctgccacc
      661 gtccacgccg cagccgcagc agccaccgcg gctgcacaca gcttccctct gtccttcgcg
      721 ggggcattcc ccatgcttcc cccaaacgca gcagcagcag tggccgcggc cacagccatc
      781 agcccgccct tgtcagtatc agccacgtcc agtcctcagc agaccagcag tggaacaaac
      841 aataaacctt accgaccctg ggggacagaa gttggagctt tttagtaatg aactagtccg
      901 taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga cgattcttca
      961 cagatccgcg aacacat
//
