LOCUS       sequence-282663-        2465 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2465)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2465)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1509
FEATURES             Location/Qualifiers
     source          1..2465
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggctgtgtg gatacaagct cagcagctcc aaggagaagc ccttcatcag
       61 atgcaagcgt tatatggcca gcattttccc attgaggtgc ggcattattt atcccagtgg
      121 attgaaagcc aagcatggga ctcagtagat cttgataatc cacaggagaa cattaaggcc
      181 acccagctcc tggagggcct ggtgcaggag ctgcagaaga aggcagagca ccaggtgggg
      241 gaagatgggt ttttactgaa gatcaagctg gggcactatg ccacacagct ccagaacacg
      301 tatgaccgct gccccatgga gctggtccgc tgcatccgcc atatattgta caatgaacag
      361 aggttggtcc gagaagccaa caatggtagc tctccagctg gaagccttgc tgatgccatg
      421 tcccagaaac acctccagat caaccagacg tttgaggagc tgcgactggt cacgcaggac
      481 acagagaatg agttaaaaaa gctgcagcag actcaggagt acttcatcat ccagtaccag
      541 gagagcctga ggatccaagc tcagtttggc ccgctggccc agctgagccc ccaggagcgt
      601 ctgagccggg agacggccct ccagcagaag caggtgtctc tggaggcctg gttgcagcgt
      661 gaggcacaga cactgcagca gtaccgcgtg gagctggccg agaagcacca gaagaccctg
      721 cagctgctgc ggaagcagca gaccatcatc ctggatgacg agctgatcca gtggaagcgg
      781 cggcagcagc tggccgggaa cggcgggccc cccgagggca gcctggacgt gctacagtcc
      841 tggtgtgaga agttggccga gatcatctgg cagaaccggc agcagatccg cagggctgag
      901 cacctctgcc agcagctgcc catccccggc ccagtggagg agatgctggc cgaggtcaac
      961 gccaccatca cggacattat ctcagccctg gtgaccagca cgttcatcat tgagaagcag
     1021 cctcctcagg tcctgaagac ccagaccaag tttgcagcca ctgtgcgcct gctggtgggc
     1081 gggaagctga acgtgcacat gaaccccccc caggtgaagg ccaccatcat cagtgagcag
     1141 caggccaagt ctctgctcaa gaacgagaac acccgcaatg attacagtgg cgagatcttg
     1201 aacaactgct gcgtcatgga gtaccaccaa gccacaggca cccttagtgc ccacttcagg
     1261 aatatgtccc tgaaacgaat taagaggtca gaccgtcgtg gggcagagtc ggtgacagaa
     1321 gaaaaattta caatcctgtt tgaatcccag ttcagtgttg gtggaaatga gctggttttt
     1381 caagtcaaga ccctgtccct gccagtggtg gtgatcgttc atggcagcca ggacaacaat
     1441 gcgacggcca ctgttctctg ggacaatgct tttgcagagc ctggcagggt gccatttgcc
     1501 gtgcctgaca aagtgctgtg gccacagctg tgtgaggcgc tcaacatgaa attcaaggcc
     1561 gaagtgcaga gcaaccgggg cctgaccaag gagaacctcg tgttcctggc gcagaaactg
     1621 ttcaacaaca gcagcagcca cctggaggac tacagtggcc tgtctgtgtc ctggtcccag
     1681 ttcaacaggg agaatttacc aggacggaat tacactttct ggcaatggtt tgacggtgtg
     1741 atggaagtgt taaaaaaaca tctcaagcct cattggaatg atggggccat tttggggttt
     1801 gtaaacaagc aacaggccca tgacctactc attaacaagc cagatgggac cttcctcctg
     1861 agattcagtg actcagaaat tggcggcatc accattgctt ggaagtttga ttctcaggaa
     1921 agaatgtttt ggaatctgat gccttttacc accagagact tctccattcg gtccctagcc
     1981 gaccgcttgg gagacttgaa ttaccttatc tacgtgtttc ctgatcggcc aaaagatgaa
     2041 gtatactcca aatactacac accagttccc tgcgagtctg ctactgctaa agctgttgat
     2101 ggatacgtga agccacagat caagcaagtg gtccctgagt ttgtgaacgc atctgcagat
     2161 gccgggggcg gcagcgccac gtacatggac caggccccct ccccagctgt gtgtccccag
     2221 gctcactata acatgtaccc acagaaccct gactcagtcc ttgacaccga tggggacttc
     2281 gatctggagg acacaatgga cgtagcgcgg cgtgtggagg agctcctggg ccggccaatg
     2341 gacagtcagt ggatcccgca cgcacaatcg tagtaatgaa ctagtccgta acttgaaagt
     2401 atttcgattt cttggcttta tatatcttgt ggaaaggacg attatcgcga gtgactctac
     2461 atacg
//
