LOCUS       sequence-282666-        2393 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2393)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2393)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1511
FEATURES             Location/Qualifiers
     source          1..2393
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcgggctg gatccaggcc cagcagctgc agggagacgc gctgcgccag
       61 atgcaggtgc tgtacggcca gcacttcccc atcgaggtcc ggcactactt ggcccagtgg
      121 attgagagcc agccatggga tgccattgac ttggacaatc cccaggacag agcccaagcc
      181 acccagctcc tggagggcct ggtgcaggag ctgcagaaga aggcggagca ccaggtgggg
      241 gaagatgggt ttttactgaa gatcaagctg gggcactacg ccacgcagct ccagaaaaca
      301 tatgaccgct gccccctgga gctggtccgc tgcatccggc acattctgta caatgaacag
      361 aggctggtcc gagaagccaa caattgcagc tctccggctg ggatcctggt tgacgccatg
      421 tcccagaagc accttcagat caaccagaca tttgaggagc tgcgactggt cacgcaggac
      481 acagagaatg agctgaagaa actgcagcag actcaggagt acttcatcat ccagtaccag
      541 gagagcctga ggatccaagc tcagtttgcc cagctggccc agctgagccc ccaggagcgt
      601 ctgagccggg agacggccct ccagcagaag caggtgtctc tggaggcctg gttgcagcgt
      661 gaggcacaga cactgcagca gtaccgcgtg gagctggccg agaagcacca gaagaccctg
      721 cagctgctgc ggaagcagca gaccatcatc ctggatgacg agctgatcca gtggaagcgg
      781 cggcagcagc tggccgggaa cggcgggccc cccgagggca gcctggacgt gctacagtcc
      841 tggtgtgaga agttggccga gatcatctgg cagaaccggc agcagatccg cagggctgag
      901 cacctctgcc agcagctgcc catccccggc ccagtggagg agatgctggc cgaggtcaac
      961 gccaccatca cggacattat ctcagccctg gtgaccagca cattcatcat tgagaagcag
     1021 cctcctcagg tcctgaagac ccagaccaag tttgcagcca ccgtacgcct gctggtgggc
     1081 gggaagctga acgtgcacat gaatcccccc caggtgaagg ccaccatcat cagtgagcag
     1141 caggccaagt ctctgcttaa aaatgagaac acccgcaacg agtgcagtgg tgagatcctg
     1201 aacaactgct gcgtgatgga gtaccaccaa gccacgggca ccctcagtgc ccacttcagg
     1261 aacatgtcac tgaagaggat caagcgtgct gaccggcggg gtgcagagtc cgtgacagag
     1321 gagaagttca cagtcctgtt tgagtctcag ttcagtgttg gcagcaatga gcttgtgttc
     1381 caggtgaagg gcagggtgcc atttgccgtg cctgacaaag tgctgtggcc gcagctgtgt
     1441 gaggcgctca acatgaaatt caaggccgaa gtgcagagca accggggcct gaccaaggag
     1501 aacctcgtgt tcctggcgca gaaactgttc aacaacagca gcagccacct ggaggactac
     1561 agtggcctgt ccgtgtcctg gtcccagttc aacagggaga acttgccggg ctggaactac
     1621 accttctggc agtggtttga cggggtgatg gaggtgttga agaagcacca caagccccac
     1681 tggaatgatg gggccatcct aggttttgtg aataagcaac aggcccacga cctgctcatc
     1741 aacaagcccg acgggacctt cttgttgcgc tttagtgact cagaaatcgg gggcatcacc
     1801 atcgcctgga agtttgactc cccggaacgc aacctgtgga acctgaaacc attcaccacg
     1861 cgggatttct ccatcaggtc cctggctgac cggctggggg acctgagcta tctcatctat
     1921 gtgtttcctg accgccccaa ggatgaggtc ttctccaagt actacactcc tgtgctggct
     1981 aaagctgttg atggatatgt gaaaccacag atcaagcaag tggtccctga gtttgtgaat
     2041 gcatctgcag atgctggggg cagcagcgcc acgtacatgg accaggcccc ctccccagct
     2101 gtgtgccccc aggctcccta taacatgtac ccacagaacc ctgaccatgt actcgatcag
     2161 gatggagaat tcgacctgga tgagaccatg gatgtggcca ggcacgtgga ggaactctta
     2221 cgccgaccaa tggacagtct tgactcccgc ctctcgcccc ctgccggtct tttcacctct
     2281 gccagaggct ccctctcata gtaatgaact agtccgtaac ttgaaagtat ttcgatttct
     2341 tggctttata tatcttgtgg aaaggacgat acaaggcaca cgtgtctcac gtc
//
