LOCUS       sequence-28267-e         754 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 754)
  AUTHORS   Ebert MS, Neilson JR, Sharp PA
  TITLE     MicroRNA sponges: competitive inhibitors of small RNAs in mammalian 
            cells.
  JOURNAL   Nat Methods. 2007 Sep . 4(9):721-6.
  PUBMED    17694064
REFERENCE   2  (bases 1 to 754)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2007 Sep . 4(9):721-6."
COMMENT     Sequence Label: CMV-d2eGFP-20
FEATURES             Location/Qualifiers
     source          1..754
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     153..174
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(569..586)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    575..686
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 atccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc
       61 cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcac ccagtccgcc
      121 ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc
      181 gccgggatca ctctcggcat ggacgagctg tacaagaagc ttagccatgg cttcccgccg
      241 gaggtggagg agcaggatga tggcacgctg cccatgtctt gtgcccagga gagcgggatg
      301 gaccgtcacc ctgcagcctg tgcttctgct aggatcaatg tgtaggcggc cgcctcgagc
      361 cggtacctgc actcgcgcac tttaccggta cctgcactcg cgcactttac cggtacctgc
      421 actcgcgcac tttaccggta cctgcactcg cgcactttac cggtacctgc actcgcgcac
      481 tttaccggta cctgcactcg cgcactttac cggtacctgc actcgcgcac tttaccggat
      541 cgcgggcccg tttaaacccg ctgatcagcc tcgactgtgc cttctagttg ccagccatct
      601 gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt
      661 tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg
      721 ggtggggtgg ggcaaggaca gcangggggg agga
//
