LOCUS       sequence-282681-        1814 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1814)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1814)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1535
FEATURES             Location/Qualifiers
     source          1..1814
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tgggtgtgaa aacatttact catagctcct cttcccacag ccaggaaatg
       61 cttggaaagc taaatatgct gcgaaatgat ggacattttt gtgatatcac tattcgtgtc
      121 caggacaaaa tcttccgggc acataaggtg gtactagcag cttgcagtga tttctttcgc
      181 accaaacttg taggccaagc cgaggatgag aacaagaatg tgttggatct gcatcatgtt
      241 acagtgactg gctttatacc tcttttagaa tatgcttaca cagccactct atcaattaac
      301 acagaaaata ttattgatgt tctagcagca gccagctata tgcaaatgtt cagtgttgcc
      361 agcacctgct cagagttcat gaaatcaagc attttatgga atacacccaa cagccaacct
      421 gaaaagggtc tagatgctgg acaagaaaat aattctaact gcaattttac ttctcgagat
      481 gggagcattt ctcccgtgtc ctcagagtgc agtgtggtag aaagaaccat tcctgtctgc
      541 cgagaatccc ggagaaagcg caaaagctac attgttatgt ctcctgaaag tcctgtaaag
      601 tgtggcacac aaacaagctc accccaggta ttgaattctt cagcttccta ctcagaaaat
      661 agaaaccaac cagttgactc ttccttagct tttccttgga cttttccttt tggaattgat
      721 cgaaggattc agcctgagaa agttaagcaa gcagaaaata cccggacttt agaattacct
      781 ggcccatctg agaccggtag aagaatggct gattatgtga cttgtgagag cacaaaaact
      841 accttgcctt taggtaccga agaagatgtc cgggtcaaag tagaaagatt aagtgatgag
      901 gaggtccatg aggaagtgtc ccagcctgtc agtgcatctc agagttcgct gagtgatcag
      961 cagacagttc caggaagtga acaagtccaa gaggaccttc tgattagtcc acagtcttcc
     1021 tctataggct cagtagatga aggcgtttct gagggcttgc ctacacttca aagcacgtct
     1081 agcactaatg ctcctccgga tgatgatgat cgattggaaa atgttcagta tccctaccaa
     1141 ctctacattg ctccttccac cagcagtaca gagcgaccaa gtccaaatgg tcccgacaga
     1201 ccttttcagt gtccaacctg cggggtgcga ttcacccgta ttcagaacct aaagcagcac
     1261 atgctcatcc actcaggaat taaaccattt cagtgtgacc gctgtgggaa aaagttcacc
     1321 agggcttact cgctaaagat gcatcgccta aagcatgaag gtaaacgctg tttccggtgc
     1381 cagatatgta gtgccacttt cacttccttc ggggaatata aacaccacat gagggtttcc
     1441 cggcacatta tccgcaagcc tcggatttac gagtgcaaaa catgtggcgc catgttcacc
     1501 aactctggaa atttaatcgt gcacctgagg agtctgaacc atgaagcatc agagctagca
     1561 aactacttcc agagcagtga tttcctagta ccggactact taaaccagga acaagaagag
     1621 acccttgttc aatatgatct tggagaacac ggttttgaaa gcaactcctc tgttcaaatg
     1681 cctgtcattt cacagtacca ctccaaaggc aaggaaccat agtaatgaac tagtccgtaa
     1741 cttgaaagta tttcgatttc ttggctttat atatcttgtg gaaaggacga tagccctgtc
     1801 ctaaccttat agac
//
