LOCUS       sequence-282684-        2558 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2558)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2558)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1543
FEATURES             Location/Qualifiers
     source          1..2558
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcccttac tcaggtacgg ttgacattta gggatgtggc catagaattc
       61 tctcaggagg agtggaaatg cctggaccct gctcagagga tcttatacag ggacgtgatg
      121 ttggagaact actggaacct tgtttctctg ggactgtgtc attttgatat gaatattatc
      181 tccatgttgg aggaagggaa agagccctgg actgtgaaga gctgtgtgaa aatagcaaga
      241 aaaccaagaa cgccggaatg tgtcaaaggc gtggtcacag atatccctcc taaatgtaca
      301 atcaaggatt tgctaccaaa agagaagagc agtacagaag cagtattcca cacagtggtg
      361 ttggaaagac acgaaagccc tgacattgaa gacttttcct tcaaggaacc ccagaaaaat
      421 gtgcatgatt ttgagtgtca atggagagat gacacaggaa attacaaggg agtgcttatg
      481 gcccagaaag aaggtaaaag agatcaacgc gacagaagag acatagaaaa caagcttatg
      541 aacaatcagc ttggagtaag ctttcattct catctgcctg aactgcagct atttcaaggt
      601 gaggggaaaa tgtatgaatg taatcaagtt gagaagtcta ccaacaatgg ttcctcagtg
      661 tcaccacttc aacaaattcc ttctagtgtc caaacccaca ggtctaaaaa atatcatgaa
      721 cttaaccatt tttcattact cacacaaaga cgaaaagcaa acagttgtgg aaaaccttat
      781 aaatgtaatg aatgtggcaa ggcgttcact cagaattcga accttacaag tcataggaga
      841 attcatagtg gagagaagcc ttacaaatgc agtgagtgcg gcaaaacctt tactgttcgt
      901 tcaaatctaa ctattcatca ggtcatccat actggagaaa aaccttacaa atgtcatgag
      961 tgtggcaagg tcttcaggca caattcatac cttgcaactc atcggcgaat tcatactgga
     1021 gagaaacctt acaagtgtaa tgagtgtgga aaagccttta gaggacattc aaacctaact
     1081 acccatcagt taattcatac tggagaaaaa ccgttcaaat gtaatgaatg tggcaagctc
     1141 ttcactcaaa attcacacct tataagtcat tggagaattc acactggaga gaaaccttac
     1201 aagtgcaatg agtgcggcaa agcctttagt gttcgttcaa gcctagcaat ccatcagaca
     1261 atccacactg gagaaaaacc ttacaaatgt aatgaatgtg gcaaagtctt taggtacaat
     1321 tcatacctcg gaaggcatcg gagagttcat actggtgaga aaccttacaa gtgtaatgaa
     1381 tgtggcaaag ccttcagtat gcattcaaac ctagctaccc atcaggtcat ccatactgga
     1441 acaaaacctt tcaaatgcaa tgaatgcagc aaggttttca ctcaaaattc acaacttgca
     1501 aatcatcgaa gaattcatac tggagagaaa ccttacaagt gtaatgagtg tgggaaagcc
     1561 ttcagtgttc gttcaagtct gactacccat caggcaatcc attctggaga gaaaccttac
     1621 aaatgtattg aatgtggcaa gagcttcact caaaaatcac accttagaag tcatcgggga
     1681 attcattctg gagagaaacc ttacaagtgt aatgaatgtg gtaaagtctt cgctcaaaca
     1741 tcacaacttg caaggcattg gagagttcat actggagaaa aaccttacaa gtgtaatgac
     1801 tgtggcagag cctttagtga tcgttcaagc ctaacttttc atcaggcaat acatactgga
     1861 gagaaacctt acaaatgtca tgaatgcggc aaggttttta ggcacaattc ataccttgca
     1921 actcatcggc gaattcatac tggagagaaa ccttacaagt gtaatgagtg tgggaaagcc
     1981 tttagtatgc attcaaacct aactacccat aaggtcatcc atactggaga gaagccttac
     2041 aaatgtaatc aatgtggcaa ggtcttcact cagaactcac accttgcaaa tcatcaaagg
     2101 actcacaccg gagagaaacc ttaccgatgc aatgagtgtg ggaaagcctt cagtgttcgt
     2161 tcaagcctaa ccacccatca ggcaatccat actgggaaaa aaccttacaa atgtaatgaa
     2221 tgtggcaagg tctttactca aaatgctcac ctggcaaatc accgaagaat tcatactggg
     2281 gagaaacctt acaggtgtac agagtgtggg aaagccttta gggtaagatc aagtctaact
     2341 acccatatgg caatccacac tggagaaaag cgttacaaat gtaatgagtg tggcaaggtc
     2401 ttcaggcaga gttcaaatct tgcaagtcat cacagaatgc ataccggaga gaaaccttac
     2461 aaatagtaat gaactagtcc gtaacttgaa agtatttcga tttcttggct ttatatatct
     2521 tgtggaaagg acgactaaag gattcaggat ccgcttta
//
