LOCUS       sequence-282693-        1598 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1598)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1598)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1564
FEATURES             Location/Qualifiers
     source          1..1598
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggaactgtt gacattcagg gatgtgacca tagaattctc tctggaggag
       61 tgggagtttc tgaaccctgc tcagcagagt ttgtatagga aagtgatgct agagaactac
      121 agaaacctgg tctctctggg tcttactgtt tctaagccag aactgattag ccgtctggag
      181 caaagacagg agccctggaa tgtgaagaga catgagacca tagccaaacc cccagctatg
      241 tcttctcatt acactgaaga ccttttgcca gaacagtgca tgcaagattc attccaaaaa
      301 gtgatactga gaagatatgg aagctgtgga cttgaggatt tacacttaag gaaggatggg
      361 gaaaatgtgg gtgagtgtaa ggatcaaaaa gaaatttata atggacttaa ccaatgtttg
      421 tcaactctac ctagcaaaat tttcccatat aataaatgtg tgaaagtctt tagtaaatca
      481 tcaaatctaa atagagaaaa cataagacat actacagaga aacttttcaa atgtatgcaa
      541 tgtggcaaag tctttaaatc tcactcaggc ctttcttatc ataagataat tcacactgaa
      601 gagaaactct gcatatgtga ggaatgtggc aaaaccttta agtggttctc ataccttact
      661 aaacataaga gaattcacac tggagagaaa ccatacaaat gtgaagaatg tggcaaagct
      721 tttaactggt gctcgagtct tactaaacat aagagaatcc atactggtga gaaaccctac
      781 aaatgtgaag aatgtggaaa agcctttcac tggtgttcac cctttgttag acataagaaa
      841 attcatacag gagaaaaacc ctatacatgt gaagactgtg gcagagcgtt taaccggcac
      901 tcacatctca ccaaacataa gacaattcac actggaaaga aaccctacaa atgtaaagaa
      961 tgtgggaaag cctttaacca ctgctcacta cttactatac atgagagaac ccatacggga
     1021 gagaaaccct ataaatgtga agaatgtggc aaagctttta actcatcatc aattcttact
     1081 gaacataagg taattcatag cggagagaaa ccctacaaat gtgaaaaatg tgacaaagtc
     1141 tttaagaggt tctcatacct tactaaacac aagagaattc acactggaga gaaaccctac
     1201 aaatgtgaag aatgtggcaa agcttttaac tggtcctcaa tccttactga acataagaga
     1261 attcatactg gagagaaacc ctacaactgt gaagaatgtg gaaaagcctt taatcggtgc
     1321 tcacacctta ctagacataa gaaaattcat actgccgtca aacgctataa atgtgaagaa
     1381 tgtggcaaag cttttaaacg gtgctcacat cttaatgaac ataagagagt tcaaagagga
     1441 gagaaatcct gcaagtataa aaaatgtggg gaagctttta atcactgctc aaaccttact
     1501 acgtagtaat gaactagtcc gtaacttgaa agtatttcga tttcttggct ttatatatct
     1561 tgtggaaagg acgaatgtca tgtctcacat gctgtcta
//
