LOCUS       sequence-28270-e         851 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 851)
  AUTHORS   Ebert MS, Neilson JR, Sharp PA
  TITLE     MicroRNA sponges: competitive inhibitors of small RNAs in mammalian 
            cells.
  JOURNAL   Nat Methods. 2007 Sep . 4(9):721-6.
  PUBMED    17694064
REFERENCE   2  (bases 1 to 851)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2007 Sep . 4(9):721-6."
COMMENT     Sequence Label: CMV-d2eGFP-20pf
FEATURES             Location/Qualifiers
     source          1..851
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     132..153
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(410..427)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    416..640
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(773..792)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg
       61 ctgctgcccg acaaccacta cctgagcacc cagtccgccc tgagcaaaga ccccaacgag
      121 aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg
      181 gacgagctgt acaagaagct tagccatggc ttcccgccgg aggtggagga gcaggatgat
      241 ggcacgctgc ccatgtcttg tgcccaggag agcgggatgg accgtcaccc tgcagcctgt
      301 gcttctgcta ggatcaatgt gtaggcggcc gcctcgagct acctgcacta taagcacttt
      361 actacctgca ctataagcac tttagggccc gtttaaaccc gctgatcagc ctcgactgtg
      421 ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa
      481 ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt
      541 aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa
      601 gacaatagca ggcatgctgg ggatgcggtg ggctctatgg cttctgangc ggaaagaacc
      661 agctggggct ctagggggta tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt
      721 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctancgcc cgctcctttc
      781 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg
      841 gggntcctta g
//
