LOCUS       sequence-282705-        3815 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3815)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 3815)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1572
FEATURES             Location/Qualifiers
     source          1..3815
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tgggggatat gaagacccct gattttgatg acctccttgc tgcctttgac
       61 atccctgaca ttgatgcgaa tgaagccatc cattctgggc cagaagaaaa tgaggggcct
      121 ggaggcccag ggaagccaga accaggtgta ggaagtgaat ctgaagacac agcagcagcc
      181 tctgctgggg atggccctgg agttccagcc caggcctctg accatggcct gccaccgcca
      241 gacatttctg tagtcagtgt cattgtcaag aacactgtgt gtcccgagca gtctgaggcc
      301 ctggctggag gctcagcagg agacggggcc caggctgctg gggtaactaa agaagggcct
      361 gtggggcctc atcgaatgca gaatggtttt gggagccctg aaccttccct cccaggaact
      421 ccccactctc ctgctcctcc cagtgggggc acctggaaag aaaaaggcat ggaaggcaaa
      481 actcccttgg acctgtttgc tcattttggc cctgagccag gggaccactc agatccgctg
      541 cctccctctg caccctctcc cactcgggag ggggctctga ccccgcctcc tttcccctct
      601 tcctttgagc tggcccagga gaatggccca ggcatgcagc cacctgtttc ttccccacca
      661 ttgggggcct tgaagcagga gagctgcagc ccccatcatc cccaggtcct agcccaacaa
      721 ggctcaggct ccagccctaa ggccacggac atccctgcca gtgcctcgcc tcccccagtt
      781 gctggggtgc ccttcttcaa gcagtctcca gggcaccaga gccctcttgc ctcccccaaa
      841 gtgcccgtct gtcagccctt gaaggaagaa gatgatgatg aggggccagt ggacaagtct
      901 tccccaggaa gtccccagag tccctctagt ggggccgagg ctgcagatga ggacagcaat
      961 gactcccctg cctccagctc ctctaggcct cttaaggtgc ggatcaagac cattaaaaca
     1021 tcctgcggga atatcacaag gactgtaact caggtcccct cagatcctga tccacctgcc
     1081 cccttggctg agggggcctt cttggctgag gctagcctct tgaagctgtc ccctgcaaca
     1141 cctacttctg agggtccaaa ggtggtgagc gtacagttgg gtgatggtac aaggctgaaa
     1201 ggcactgtgc tgcctgtggc caccatccag aacgccagta ctgccatgct gatggcagcc
     1261 agtgtggctc gcaaggctgt ggtgctgcct ggggggactg ccaccagccc taagatgatt
     1321 gctaagaacg tgctaggcct ggtgccccaa gccctgccta aggctgacgg gcgggcaggg
     1381 ctggggactg ggggacagaa ggtgaatggt gcctcggtgg tgatggtgca accttcaaag
     1441 acagctactg ggccaagtac agggggcggc acagtgatat cacggaccca gtccagcctg
     1501 gtggaggcct tcaacaagat cctcaacagc aagaacctgc tccctgccta taggccaaac
     1561 ctgagcccac cagctgaggc tgggctggcc ctgcctccca ccggctaccg ctgcctggag
     1621 tgtggggatg ccttctcatt ggagaagagc ctggcacggc actatgaccg tcggagcatg
     1681 cgcatcgagg tcacctgcaa ccactgcgcc cgccgcctgg tcttcttcaa caagtgcagc
     1741 ctgctcctgc atgcacgtga acacaaggac aaggggctcg tcatgcagtg ctcacatttg
     1801 gtcatgaggc ctgtagccct tgaccagatg gtggggcagc cggacatcac accgctgctg
     1861 cctgtagctg tcccacctgt ctctggacct ctggccttgc ctgccttggg caagggtgag
     1921 ggggccatca cctcctctgc cattactaca gttgctgctg aggcccctgt cctgccgctc
     1981 tccacagagc cgcctgctgc cccggccacc tctgcttaca catgctttcg ctgcctggag
     2041 tgcaaggaac agtgccggga caaggctggc atggcagctc acttccagca gctcggcccc
     2101 cctgcccctg gggccaccag caatgtgtgc ccaacctgcc ccatgatgct ccccaatcgc
     2161 tgcagcttca gcgcccacca gcgcatgcat aagaatcgac ccccccatgt ctgtcctgag
     2221 tgtgggggca acttcctgca agccaatttt cagacccatc tccgggaggc ctgtctgcac
     2281 gtctctcgcc gtgtaggata caggtgcccc agctgttcag tggtgtttgg gggtgtgaac
     2341 tccatcaagt cccacatcca gacgtcgcac tgcgaggttt tccacaagtg ccccatctgc
     2401 cccatggcct tcaagtctgg gccaagtgcc catgcccacc tctactccca gcatcccagc
     2461 ttccaaactc agcaggccaa gctgatctac aagtgcgcca tgtgcgacac agtcttcact
     2521 cacaaacccc tcctctcctc acacttcgac cagcacttgc tgccccagcg tgtcagtgtc
     2581 tttaagtgcc cgtcttgtcc tctgctcttt gcccaaaaaa ggaccatgct ggaacatctc
     2641 aagaacaccc atcagtctgg gcgcttggag gagactgctg ggaaaggggc cgggggtgcc
     2701 ctgctgaccc ccaagactga gcctgaggag ctggctgttt ctcagggagg ggcagcccct
     2761 gctactgagg agtcgtcttc atcttcagaa gaggaggaag tacccagctc ccctgagccc
     2821 ccccgtccag ccaaacggcc tcggcgggaa ctagggagca aaggcctcaa gggtgggggt
     2881 ggggggcctg gaggctggac ctgtggcctg tgtcactcct ggttccctga gcgtgatgaa
     2941 tacgtggccc acatgaagaa ggagcatggc aagtcagtga aaaagttccc ctgtcgcctg
     3001 tgtgagcgct ccttctgctc cgcccccagc ctgaggcgcc atgtcagagt taatcacgag
     3061 ggcatcaagc gagtttaccc ctgcaggtat tgcacagagg gaaaacgcac cttcagcagc
     3121 cgcctgatcc tagagaaaca tgtccaggtc cggcacggct tgcagcttgg ggcccagtcc
     3181 cctggccggg ggaccacctt ggctcggggt tccagtgcca gagcccaggg gccaggtcgg
     3241 aaacgccgcc agtcttctga ctcttgcagt gaggagcctg acagcacgac accgccagcc
     3301 aagtccccca ggggcggacc tggatctgga ggccatggcc ctctgcgcta ccggagcagc
     3361 agctccacag aacagagcct catgatgggg ttgagggtgg aggatggtgc ccagcagtgc
     3421 ctcgactgtg gcttgtgctt tgcctcccct ggctccctga gccgacaccg tttcatcagc
     3481 cacaagaaga gacggggtgt gggtaaagcc agtgccctgg ggctggggga tggggaggaa
     3541 gaggcccctc catcaaggtc tgaccccgat ggtggagact cacccctgcc tgcttctgga
     3601 ggcccactga cctgtaaggt ctgtggcaag agctgcgaca gccctctaaa cctcaagacc
     3661 cacttccgca cgcatggcat ggcgttcatc agggctcggc agggggctgt tggggacaac
     3721 tagtaatgaa ctagtccgta acttgaaagt atttcgattt cttggcttta tatatcttgt
     3781 ggaaaggacg aagcgggagg agtaacgtag cgcag
//
