LOCUS       sequence-282708-        2291 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2291)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2291)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1580
FEATURES             Location/Qualifiers
     source          1..2291
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcagcccc tgacctggcc cacggaggtc atgtttctag ggactcagtc
       61 tgccttcatg aagaacagac acaggcagca gggatggtgg ctggctggct gataaattgt
      121 taccaggacg cggtgacctt tgacgacgtg gctgtggact tcacccagga ggaatggact
      181 ttactggacc catctcagag agacctctac agagatgtga tgctggaaaa ctacgagaac
      241 ctggcctcag tagaatggcg acttaaaacc aaagggccag cacttcggca ggatagatct
      301 tggttcagag catcaaatga gacacagacg gcaagaagcc acaatggagg gcagctctgt
      361 gaccgcacgc agtgtggaga agctttcagt gaacactcag gcctcagcac acacgtgaga
      421 actcaaaata caggagacag ttgtgtgtct aatcattatg aaagggactt ttttattcca
      481 tgccagaaaa ccttgttcaa aattggagag cagttttccg tgttgggtca gtgtggaaaa
      541 gccttcagct ctactccaaa tgttgtttcc cagcaagcat gcactcggga cagatctctt
      601 gactacagca gctgtgggga agtgttcctt aatcagtcat accttcaggc acgtgcggga
      661 agtcacaacg gagaagaaac atggaaatgg aagccgtgtg ggaaagctct aactcactcc
      721 atgggctgcg ccacacctgt tgaaatgcat gccgtcagga atccccacgt atgtagggaa
      781 tgtgggaagg cctttaggta cactgcctac cttactggtc gcgtgcaagt ccaccctggg
      841 gaaaagccct gtgaattgga agaatgtgga aaagcctccc ctgtttcttc cagcctaact
      901 caacatgtaa gaattcatgc tgcagagaaa ccctgtgaat gtaaagaatg cggaaaagcc
      961 ttcactggac tctcaggtct ttctaaacac gtccaaacag accctggaca gaagccctat
     1021 gaatgtaagg actgtgggaa agcctgcggt gggttttatc tactgaatga gcatggaaaa
     1081 actcacacga gggagaagcc ctttgcatgt gtggtttgcg gaaaatattt tagaaattcc
     1141 tcatgcctta ataatcatgt tcgaattcac actggaataa aaccctatac atgcagctac
     1201 tgtgggaagg ccttcactgt gcgctgtggc cttactagac acgtacgaac acacacgggc
     1261 gagaagccat acacgtgtaa ggactgcggg aaagccttct gtacatcctc gggccttact
     1321 gagcatgtaa ggactcacac tggagagaaa ccatatgaat gtaaagattg tgggaaatcc
     1381 ttcactgttt cttcaagcct gactgagcac gcgagaatcc ataccggaga gaaaccctac
     1441 gaatgtaagc agtgtggcaa agccttcaca gggcgctcag gcctcactaa acacatgcgg
     1501 acacacaccg gggagaagcc ctatgaatgt aaggactgtg ggaaagccta caatagggtt
     1561 tatctactga atgagcatgt gaaaactcac acagaggaga agccctttat atgtacggta
     1621 tgcaggaaat ccttcagaaa ttcctcgtgc ctgaataagc acattcagat tcacactgga
     1681 ataaaacctt atgaatgtaa ggactgtggg aaaacattca ctgtttcttc gagcctaacc
     1741 gagcacatac gaactcacac tggagagaaa ccttatgaat gtaaagtatg cggaaaggcc
     1801 ttcaccacat cctcacacct tatcgtgcac ataagaaccc acaccggtga gaaaccctac
     1861 atatgtaagg agtgtgggaa agcctttgct tcctcctcac accttatcga acacagaagg
     1921 actcacacag gagagaaacc ttacatatgt aacgagtgtg ggaaagcctt ccgtgcctcc
     1981 tctcacctgc ataaacatgg aagaattcac actgggcaga aaccctataa atgtaaggaa
     2041 tgtgggaaag catacaatag gttttatcta ctaaaagaac atttaaaaac ttacactgaa
     2101 gagcaggttt ttgtatgtaa ggactgtgga aaatctttta agaattcctc atgccttaac
     2161 catcacactc aaattcacac tgatgagaaa cctttctagt aatgaactag tccgtaactt
     2221 gaaagtattt cgatttcttg gctttatata tcttgtggaa aggacgatca gaaggccatg
     2281 ctgcggtcat c
//
