LOCUS       sequence-282711-        1448 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1448)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1448)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1585
FEATURES             Location/Qualifiers
     source          1..1448
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      8..17
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcggcggc tgagccgatg ggcccggcac aggttcccat gaactcagaa
       61 gtaattgtgg accctataca ggggcaggtg aactttgagg acgtgttcgt gtacttctcc
      121 caggaggagt gggtgctcct tgatgaggct cagaggctcc tgtaccgtga tgtgatgctg
      181 gagaactttg cacttatggc ctctctgggt tgttggcatg gaatggagga tgaagagata
      241 ccttttgagc agagcttttc tataggaatg tcacagatca ggattcccaa gggaggtcct
      301 tctactcaga aggcttaccc ctgtgggaca tgtggcctgg tcttaaaaga cattttgcac
      361 ttggctgagc accaggaaac acacccaggg cagaaaccat acatgtgtgt gctgtgtggg
      421 aaacagttct gcttcagtgc aaaccttcac cagcaccaga agcagcacag tggagagaaa
      481 ccctttagaa gtgataagag caggcccttt cttctgaaca actgtgctgt gcaatcaatg
      541 gagatgtctt ttgtgacagg ggaggcttgt aaggacttcc tagccagctc aagcattttc
      601 gagcaccatg cccctcacaa tgagtggaag ccacacagca acaccaagtg tgaggaggcc
      661 tctcactgtg gaaaaaggca ttacaaatgc agtgaatgtg ggaaaacctt cagccgcaaa
      721 gactcacttg ttcaacacca gagagtccac actggagaaa ggccttatga gtgcggtgaa
      781 tgtgggaaaa cctttagccg caaacccata cttgctcagc accagagaat ccacactgga
      841 gaaatgcctt atgagtgtgg catatgtggg aaagttttta atcatagctc taaccttatt
      901 gtacatcaaa gagtacacac tggagcaagg ccttacaagt gcagtgaatg tgggaaagcc
      961 tatagtcaca aatctacact tgttcagcat gagagtatcc atactggaga aaggccatat
     1021 gagtgcagcg aatgtggaaa atactttggt cacaaataca gactcattaa acattggagt
     1081 gttcatactg gagcaaggcc ttatgagtgc atcgcatgtg ggaagttctt tagccaaagc
     1141 tctgacctta ttgcacatca aagagttcat aatggtgaaa agccttatgt gtgcagtgaa
     1201 tgtggaaaag cctttagcca caaacatgta cttgttcagc atcatagaat tcacactgga
     1261 gaaaggccat ataagtgcag tgaatgtggg aaggccttca ggcagagggc ttccctcatc
     1321 cgccactgga aaattcacac tggagaaagg ccttagtaat gaactagtcc gtaacttgaa
     1381 agtatttcga tttcttggct ttatatatct tgtggaaagg acgatgcggc agatttcatc
     1441 ttaccagg
//
