LOCUS       sequence-282714-        1571 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1571)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1571)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1586
FEATURES             Location/Qualifiers
     source          1..1571
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      8..17
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcggcggc tgagccgatg ggcccggcac aggttcccat gaactcagaa
       61 gtaattgtgg accctataca ggggcaggtg aactttgagg acgtgttcgt gtacttctcc
      121 caggaggagt gggtgctcct tgatgaggct cagaggctcc tgtaccgtga tgtgatgctg
      181 gagaactttg cacttatggc ctctctggga catacatctt tcatgtccca catagttgcc
      241 tcactggtga tgggtagtga gccctgggta cctgactggg tggacatgac cctagctgtt
      301 gccacagaga ctccaggtgg gagtgaccct ggttgttggc atggaatgga ggatgaagag
      361 ataccttttg agcagagctt ttctatagga atgtcacaga tcaggattcc caagggaggt
      421 ccttctactc agaaggctta cccctgtggg acatgtggcc tggtcttaaa agacattttg
      481 cacttggctg agcaccagga aacacaccca gggcagaaac catacatgtg tgtgctgtgt
      541 gggaaacagt tctgcttcag tgcaaacctt caccagcacc agaagcagca cagtggagag
      601 aaacccttta gaagtgataa gagcaggccc tttcttctga acaactgtgc tgtgcaatca
      661 atggagatgt cttttgtgac aggggaggct tgtaaggact tcctagccag ctcaagcatt
      721 ttcgagcacc atgcccctca caatgagtgg aagccacaca gcaacaccaa gtgtgaggag
      781 gcctctcact gtggaaaaag gcattacaaa tgcagtgaat gtgggaaaac cttcagccgc
      841 aaagactcac ttgttcaaca ccagagagtc cacactggag aaaggcctta tgagtgcggt
      901 gaatgtggga aaacctttag ccgcaaaccc atacttgctc agcaccagag aatccacact
      961 ggagaaatgc cttatgagtg tggcatatgt gggaaagttt ttaatcatag ctctaacctt
     1021 attgtacatc aaagagtaca cactggagca aggccttaca agtgcagtga atgtgggaaa
     1081 gcctatagtc acaaatctac acttgttcag catgagagta tccatactgg agaaaggcca
     1141 tatgagtgca gcgaatgtgg aaaatacttt ggtcacaaat acagactcat taaacattgg
     1201 agtgttcata ctggagcaag gccttatgag tgcatcgcat gtgggaagtt ctttagccaa
     1261 agctctgacc ttattgcaca tcaaagagtt cataatggtg aaaagcctta tgtgtgcagt
     1321 gaatgtggaa aagcctttag ccacaaacat gtacttgttc agcatcatag aattcacact
     1381 ggagaaaggc catataagtg cagtgaatgt gggaaggcct tcaggcagag ggcttccctc
     1441 atccgccact ggaaaattca cactggagaa aggccttagt aatgaactag tccgtaactt
     1501 gaaagtattt cgatttcttg gctttatata tcttgtggaa aggacgacca acggcccgaa
     1561 tatacttctt t
//
