LOCUS       sequence-28272-e        1038 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1038)
  AUTHORS   Ebert MS, Neilson JR, Sharp PA
  TITLE     MicroRNA sponges: competitive inhibitors of small RNAs in mammalian 
            cells.
  JOURNAL   Nat Methods. 2007 Sep . 4(9):721-6.
  PUBMED    17694064
REFERENCE   2  (bases 1 to 1038)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2007 Sep . 4(9):721-6."
COMMENT     Sequence Label: CMV-d2eGFP-21
FEATURES             Location/Qualifiers
     source          1..1038
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     159..180
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(575..592)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    581..805
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 cttcagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag
       61 aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcacccag
      121 tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg
      181 accgccgccg ggatcactct cggcatggac gagctgtaca agaagcttag ccatggcttc
      241 ccgccggagg tggaggagca ggatgatggc acgctgccca tgtcttgtgc ccaggagagc
      301 gggatggacc gtcaccctgc agcctgtgct tctgctagga tcaatgtgta ggcggccgcc
      361 tcgagccggt caacatcagg acataagcta ccggtcaaca tcaggacata agctaccggt
      421 caacatcagg acataagcta ccggtcaaca tcaggacata agctaccggt caacatcagg
      481 acataagcta ccggtcaaca tcaggacata agctaccggt caacatcagg acataagcta
      541 ccggatcgcg ggcccgttta aacccgctga tcagcctcga ctgtgccttc tagttgccag
      601 ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact
      661 gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt
      721 ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat
      781 gctggggatg cggtgggctc tatggcttct gagcggaaag accagctggg gctctagggg
      841 gtatccccac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgt taacgcgcag
      901 cgtgaccgct acacttgcca gcgccctanc gcccgctctt tcgctttctc ccttcctttc
      961 tcgcacgtcg ccgntttccc ggtcagctct aantcggggc tccctttagg ttccgattta
     1021 ngcttacggc acctcgac
//
