LOCUS       sequence-282720-        5819 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 5819)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 5819)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1605
FEATURES             Location/Qualifiers
     source          1..5819
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(1036..1047)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 accgccacca tggaaatgga ggcaaacgac cattttaact ttactggcct tccccctgca
       61 cctgctgcct caggactgaa accctctcct tcctcagggg agggcctcta cactaacggg
      121 tctcccatga acttccccca gcaagggaaa agtttgaatg gggatgtgaa tgttaatggc
      181 ttatctactg tatctcacac tactacttca gggattttga actctgctcc ccactcctcc
      241 agcacctcac acctccatca ccccagcgtg gcctacgact gtctctggaa ctactcacag
      301 tacccatctg ccaatcctgg cagcaacctc aaggacccac cccttctctc ccagttctcg
      361 gggggacaat acccactcaa cggcatcctt gggggcagcc ggcaaccttc atccccaagt
      421 cataacacta accttcgggc tgggagccaa gagttctggg ccaacggtac ccagagtccc
      481 atggggctta actttgattc acaagaactg tatgattcct ttcctgacca gaattttgag
      541 gtgatgccca atggaccccc tagttttttc acctccccac agacttctcc tatgttggga
      601 tctagcattc aaacctttgc accctcccag gaggtaggca gtggtatcca tcctgatgag
      661 gcagcagaaa aggagatgac ttcagttgtg gcagagaatg gcactggctt ggtaggcagc
      721 ttggagctgg aagaagagca gccagaactg aagatgtgtg gctacaatgg ctctgtccct
      781 tctgtggaat cgttacacca agaggtctca gtcctggtcc ctgaccccac agtgagctgt
      841 ttagatgatc cttcacatct tcctgatcaa ctggaagaca ctccaatcct cagtgaagac
      901 tctctggagc ccttcaactc tctggcacca gagccagtga gtggaggact atatggtatt
      961 gatgacacgg agctgatggg tgcagaggac aagctgcctc ttgaggacag ccctgtgatt
     1021 tctgcccttg attgcccttc cctcaataat gctactgcct tcagtctcct ggcagatgat
     1081 agtcaaacat caacctctat ctttgccagt cccacctctc cacctgtcct aggggagtct
     1141 gtcctgcaag ataacagctt tgacctgaat aatggtagtg acgctgaaca ggaagaaatg
     1201 gaaactcaat cttcagactt cccaccatcc ctgacccagc cagctcctga tcagtcatcc
     1261 actattcagc tacatccagc aacctcacca gcagtctcgc caacaacctc cccagcagtc
     1321 tccctagtgg tttctccagc agcctcccca gaaatctctc cagaagtttg tcccgcagct
     1381 tctacagttg tctctccagc agtcttctca gtggtctctc cagcttcctc agcagtcctc
     1441 ccagcagtct ccttagaagt cccgttgacg gcttcagtga catccccaaa agcctctccc
     1501 gtaacttccc cagcagctgc ctttccaaca gcctccccag caaataagga tgtcagcagc
     1561 tttctagaaa ccactgctga cgtggaagag atcactggag aaggactcac tgcttctggt
     1621 agtggtgatg tcatgaggag acgtattgct accccagaag aagttcgtct tcccctccaa
     1681 catgggtggc ggagagaggt gcgcatcaag aagggcagcc accgatggca gggggagacc
     1741 tggtattatg gcccctgtgg gaagaggatg aagcaatttc cagaagtgat caagtacctg
     1801 agccgcaacg tggtacacag tgtccgccga gagcacttca gcttcagtcc ccgtatgcct
     1861 gttggagatt tctttgaaga aagagacacg ccagagggct tgcagtgggt gcagctctca
     1921 gcagaggaga tcccgtcgag gattcaggca attactggca aacggggtcg acctcgaaac
     1981 actgagaagg ctaagactaa ggaagtcccc aaggtgaaac ggggtcgagg tcggccacct
     2041 aaggtcaaaa tcactgagct attgaacaag acagacaacc gccccctaaa gaaactggag
     2101 gcccaagaaa cattgaatga ggaggataaa gcaaagattg ctaaaagcaa gaagaagatg
     2161 aggcagaagg ttcaacgggg agagtgtcag actactatcc aagggcaggc cagaaataag
     2221 cggaaacaag agaccaagag cttaaagcag aaggaagcta agaagaaatc caaggctgag
     2281 aaagaaaaag gaaagacaaa gcaggaaaaa ctgaaggaaa aagtcaagag ggaaaagaag
     2341 gagaaggtaa aaatgaagga aaaggaggag gtgaccaaag ccaagccagc ctgtaaagca
     2401 gataagaccc tggccacaca gaggcgcttg gaggaacggc agaggcagca gatgatcttg
     2461 gaggaaatga agaagccgac agaggatatg tgtctgactg accaccagcc cctgcctgac
     2521 ttctcacgag tccctggtct gacattgccc agtggagcct tctcagactg cttgaccatt
     2581 gtggagttcc tgcatagctt tggcaaggtg ctgggctttg atcctgccaa agatgtgcct
     2641 agcctggggg tcctgcagga gggactcctg tgtcaaggtg acagcttggg tgaggtgcaa
     2701 gacctgctgg tcaggctgct gaaggctgca ctccatgatc ctggctttcc ctcctactgt
     2761 cagtccctaa agatcttggg ggagaaggtg tctgagatcc cactgacaag agacaatgtg
     2821 tcagagatcc tgcgctgctt ccttatggca tatggagtag agccagccct ctgtgaccgc
     2881 ctgcgcaccc agccttttca ggcccagcca ccccagcaga aggctgctgt cctggccttc
     2941 cttgtgcatg agctcaatgg ctccaccctc atcatcaatg agattgacaa gactctggag
     3001 agtatgtcca gctacaggaa aaacaagtgg attgttgaag gccggctccg gaggctgaaa
     3061 actgttctgg ccaagcgaac tgggcggtct gaagtagaga tggaagggcc agaggaatgc
     3121 ctgggacgga ggcgcagttc tcggatcatg gaggagacca gtggcatgga agaagaggaa
     3181 gaagaggagt ctatagcagc tgtccctggc cgcaggggtc gaagagatgg agaggttgat
     3241 gccacagcat ctagcatccc agagctagag cgccagatag aaaaactcag caagcgtcag
     3301 cttttctttc gcaaaaagct gcttcactca tcccagatgc ttcgggcggt ctccctgggt
     3361 caggaccgct acagacgtcg ctactgggta ttgccgtatt tggctggtat ctttgtagaa
     3421 ggaacagagg ggaacttagt tcctgaggag gtgataaaga aggaaactga ctccttaaaa
     3481 gtggcagccc atgcgtcact caaccctgcc ctcttctcta tgaagatgga gttagctggc
     3541 tccaacacca ctgccagttc tcctgcccgg gcccgaggcc gacctcgaaa aactaagccc
     3601 gggtctatgc aacctaggca tcttaagtcc cctgtcaggg gtcaggattc agaacagccc
     3661 caggcccagc ttcagcctga ggctcagctt catgctcctg cccagcccca gcctcagctt
     3721 cagcttcagc ttcagtccca taaggggttc ctggagcaag aaggctcccc tttgtcactg
     3781 ggtcagagcc agcatgacct cagccagtca gccttcctgt cttggctgag ccagactcag
     3841 agccatagct ccctgttgag cagctcagtc ctcacacctg atagcagtcc gggaaaacta
     3901 gacccagctc catcacaacc cccggaggag ccagagcctg atgaggcaga atccagccct
     3961 gatcctcaag cactctggtt taacatctca gcccagatgc cctgcaatgc tgcccccaca
     4021 ccgccccctg cagtttctga ggaccaaccc actccctccc ctcagcagct tgcctcctcc
     4081 aagccaatga atagacctag tgctgccaac ccttgttctc cagtgcagtt ctcttccacg
     4141 cccttggctg ggttggcccc taagaggcga gcaggagacc ctggagaaat gccacagagt
     4201 cccacagggc tgggacagcc caaacggaga gggagacctc ccagtaagtt cttcaaacag
     4261 atggaacagc gttacctaac ccagctgaca gcccagcctg tcccacctga gatgtgctca
     4321 ggctggtggt ggatacgaga tcctgagatg ttggatgcca tgctcaaggc cctacacccc
     4381 cgaggtatcc gggagaaggc acttcacaaa caccttaaca agcacaggga cttcttgcag
     4441 gaagtctgcc tgcggccctc agctgacccc atctttgagc ccaggcaact acctgccttt
     4501 caagaaggga ttatgagctg gtcccccaaa gagaagacat acgagacaga cctagcagtg
     4561 cttcaatggg tagaggagct ggagcagcgg gttatcatgt ctgatctgca gattcggggc
     4621 tggacatgtc ctagcccaga ctctacccgt gaagacttgg cctactgtga gcacctctcc
     4681 gactcccagg aggatatcac ctggcgaggt cggggcaggg agggactggc acctcagcgt
     4741 aaaactacca accctttgga cctggctgtg atgcggctgg ctgccctgga acagaatgta
     4801 gaacggcggt acctgcggga gcccctctgg ccaactcatg aggttgtgct ggagaaggcc
     4861 ctgcttagca cacctaatgg tgcccctgag ggcaccacta cagagatatc atatgagatc
     4921 acccctcgca ttcgtgtctg gcgccagacc ctcgagcggt gccggagcgc agcccaggtg
     4981 tgcttgtgcc tgggccagct ggagaggtcc attgcctggg agaagtctgt caacaaagtg
     5041 acatgtctag tctgccggaa gggtgacaat gatgagtttc ttctgctttg tgatgggtgt
     5101 gaccgtggct gccacattta ctgccatcgt cccaagatgg aggctgtccc agaaggagat
     5161 tggttctgta ctgtctgttt ggctcagcag gtggagggag aattcactca gaagcctggt
     5221 ttcccaaagc gtggccagaa gcggaaaagt ggttattcgc tgaacttctc agagggtgat
     5281 ggccgccgac gccgggtact gttgaggggc cgagaaagcc cagcagcagg gcctcggtac
     5341 tcggaagaag ggctctcccc ctccaagcgg cggcgactct ctatgcggaa ccaccacagt
     5401 gatctcacat tttgcgagat tatcctgatg gagatggagt cccatgatgc agcctggcct
     5461 ttcctagagc ctgtgaaccc acgtttggtg agtgggtacc ggcgcatcat caaaaatcct
     5521 atggattttt ccaccatgcg ggagcggctg ctcaggggag ggtacaccag ctcagaggag
     5581 tttgcggctg atgccctcct ggtatttgac aactgccaga ctttcaacga ggatgactct
     5641 gaagtaggca aggctgggca catcatgcgc cgcttcttcg agagccgctg ggaggagttt
     5701 tatcagggaa aacaggccaa tctgtagtaa tgaactagtc cgtaacttga aagtatttcg
     5761 atttcttggc tttatatatc ttgtggaaag gacgatcctt gtaagctgag tttgtaaat
//
