LOCUS       sequence-282723-        3776 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3776)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 3776)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1646
FEATURES             Location/Qualifiers
     source          1..3776
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggaggatga atcaatttac acctgcgatc actgtcagca ggacttcgag
       61 tctctggcag acctgacgga ccaccgggcc caccgctgtc ctggagatgg tgatgacgac
      121 ccacaactct cctgggtggc ctcgtctccc tccagcaagg atgttgcgtc acccacgcag
      181 atgatcggag atggttgtga cctcggcctc ggcgaggagg aagggggcac gggcctgcca
      241 tacccttgcc agttctgcga caagtccttc atccgcttga gctacttgaa gaggcacgag
      301 cagatccaca gcgacaagct gccgttcaag tgcacctact gcagccgcct cttcaagcac
      361 aagaggagcc gtgaccggca catcaagctg catacgggcg acaagaagta tcactgccac
      421 gagtgcgagg cagccttctc ccgcagcgac cacctcaaga tccacctgaa gacccacagc
      481 tccagcaagc ccttcaagtg cactgtgtgc aagcgcggct tctcctccac cagctcgctg
      541 cagagccaca tgcaggccca caaaaagaac aaggagcatc tggccaagtc ggagaaggaa
      601 gccaagaagg acgacttcat gtgcgactac tgcgaggaca ccttcagcca gacggaggag
      661 ctggagaagc acgtgctcac ccgccacccg cagctgtccg agaaggcgga cctgcagtgc
      721 attcactgcc ctgaggtctt cgtcgacgag aacacactgc tcgcccatat ccaccaagcc
      781 cacgccaacc agaaacacaa gtgccccatg tgccctgagc agttctcctc agtggaaggt
      841 gtctactgcc acctggacag ccaccggcag cccgactcca gcaaccacag tgtcagtccc
      901 gaccctgtac tgggcagcgt ggcctccatg agcagcgcca cacccgactc cagcgcctct
      961 gtggagcgtg gctccacccc ggactccacc ttgaagccgc tgcgggggca gaagaagatg
     1021 cgggatgacg ggcagggctg gaccaaggtg gtctatagct gcccctattg ttccaagcgg
     1081 gactttaaca gcctggccgt gctggagatc cacctgaaga ccatccacgc ggacaagccc
     1141 cagcagagcc acacatgtca gatctgcctg gactccatgc ccaccctcta caacctcaac
     1201 gagcacgttc gcaagctgca caagaaccat gcctaccctg tgatgcagtt tggcaacatc
     1261 tctgccttcc actgcaacta ctgccccgag atgttcgccg acatcaatag cctgcaggag
     1321 cacatccgcg tctcccactg cggccccaac gccaacccct ctgacggtaa taatgctttc
     1381 ttctgcaacc agtgctccat gggtttcctt actgagtcct ccctcaccga gcacatccag
     1441 caggcccact gcagtgtggg cagtgccaaa ctagagtctc cggtggtgca gcccacgcag
     1501 tccttcatgg aggtctattc ctgcccctac tgcaccaact cccccatctt tggctccatc
     1561 ctgaaactca ccaagcacat caaggagaac cacaagaaca ttccactggc ccacagcaag
     1621 aagtccaagg ccgagcagag cccagtctcg tccgatgtgg aggtgtcttc cccgaagcgg
     1681 cagcggctct cagcaagcgc caactccatc tccaatgggg agtatccttg caatcaatgc
     1741 gacctcaagt tctccaactt tgagagcttc cagacccacc tgaagctgca cctggagctg
     1801 ctgctgcgga agcaagcgtg cccccagtgc aaagaggact ttgactccca ggagtccctc
     1861 ctgcagcacc tgacagtgca ttacatgacc acgtcgaccc actatgtgtg cgagagctgc
     1921 gacaagcaat tttcctcggt ggatgacctg cagaagcacc tgctggacat gcacaccttt
     1981 gtgttgtacc actgcaccct gtgtcaggag gtcttcgact ccaaggtgtc catccaggtg
     2041 cacctggcgg tgaagcacag caatgagaag aagatgtacc gctgcacggc ctgcaactgg
     2101 gacttccgca aggaggctga cctgcaggtg cacgtcaaac acagccacct gggcaacccg
     2161 gccaaggctc acaagtgcat cttctgtggg gagaccttca gcaccgaggt ggagctgcag
     2221 tgccacatca ccacacacag caagaagtat aactgtaagt tctgcagcaa ggccttccac
     2281 gccatcatcc tgctggagaa gcacctgcgg gagaagcact gtgtgtttga tgctgcgacc
     2341 gagaacggca cggccaatgg ggtaccccca atggccacca agaaagctga gcctgctgac
     2401 ctgcagggca tgctgcttaa gaaccctgag gcacctaaca gccatgaggc cagcgaggat
     2461 gacgtggacg cgtcggagcc catgtacggc tgtgacatct gtggggcggc ctacaccatg
     2521 gaggtgctgc tgcagaatca ccggctgcgg gaccacaata tccggccggg cgaggatgat
     2581 ggctcacgca agaaggctga gtttatcaag ggcagtcaca agtgcaacgt ttgttcacgg
     2641 actttcttct cggagaacgg gctacgggag cacctgcaga cgcaccgggg ccctgccaag
     2701 cactacatgt gtcccatctg tggtgagcgc ttcccttcgc tgctgacgct caccgaacac
     2761 aaggtgaccc acagcaagag cctggacacg ggcacctgtc gcatctgcaa gatgcccctg
     2821 cagagcgagg aggagtttat tgagcactgc cagatgcacc ctgacctgcg caactcactc
     2881 acgggcttcc gctgtgtggt ctgcatgcag acagtcactt ccacgcttga gctcaagatc
     2941 catggcacct tccacatgca gaagctggcg ggcagctcag cggcgtcctc ccccaatggc
     3001 caggggctgc agaagctcta caagtgcgcc ctgtgcctca aggagttccg cagcaagcag
     3061 gacctggtga agcttgacgt caatgggctg ccctacggcc tctgcgccgg ctgcatggcc
     3121 cgcagcgcca acggacaggt gggtggcctg gccccgcccg agcccgccga ccggccctgt
     3181 gccggcctcc gttgccccga gtgcagtgtc aagtttgaga gtgccgaaga cctggagagc
     3241 cacatgcagg tggaccaccg tgacctcacg ccggagacca gtgggccccg gaaaggcacc
     3301 cagacatcgc cagtgccccg gaaaaagaca taccagtgca tcaagtgcca gatgaccttc
     3361 gagaacgaga gagagatcca aatccacgtt gccaaccaca tgattgagga aggcatcaac
     3421 cacgagtgta agctgtgcaa ccagatgttc gactccccgg ccaagctcct ctgtcacctc
     3481 attgagcaca gcttcgaggg catgggcggc accttcaaat gccccgtgtg tttcacagtc
     3541 ttcgtccagg ccaacaagtt gcagcagcac atctttgccg tgcacgggca ggaggacaag
     3601 atctacgact gctcacagtg ccctcagaag ttcttcttcc agaccgagct gcagaaccac
     3661 acgatgagcc agcacgcaca gtagtaatga actagtccgt aacttgaaag tatttcgatt
     3721 tcttggcttt atatatcttg tggaaaggac gaagttgccc agacgattgg gcgtgc
//
