LOCUS       sequence-28273-e         739 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 739)
  AUTHORS   Ebert MS, Neilson JR, Sharp PA
  TITLE     MicroRNA sponges: competitive inhibitors of small RNAs in mammalian 
            cells.
  JOURNAL   Nat Methods. 2007 Sep . 4(9):721-6.
  PUBMED    17694064
REFERENCE   2  (bases 1 to 739)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2007 Sep . 4(9):721-6."
COMMENT     Sequence Label: U6+27-16
FEATURES             Location/Qualifiers
     source          1..739
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(391..408)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(391..407)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(400..422)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(636..655)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 tggctttata tatcttgtgg aaaggacgaa acaccgtgct cgcttcggca gcacatatac
       61 tagtcgacgc ggccgcctcg agccggcgcc aatattctat gctgctaccg gcgccaatat
      121 tctatgctgc taccggcgcc aatattctat gctgctaccg gcgccaatat tctatgctgc
      181 taccggcgcc aatattctat gctgctaccg gcgccaatat tctatgctgc taccggcgcc
      241 aatattctat gctgctaccg gcgccaatat tctatgctgc taccggcgcc aatattctat
      301 gctgctaccg gatcgcgggc ccgtttaaac ccgctctaga gcggacttcg gtccgctttt
      361 tactaggacc tgcaggcatg caagcttggc actggccgtc gttttacaac gtcgtgactg
      421 ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg
      481 gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg
      541 cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag
      601 cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt
      661 tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt
      721 ccgatttagt gctttacgg
//
