LOCUS       sequence-282735-        1799 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1799)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1799)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1668
FEATURES             Location/Qualifiers
     source          1..1799
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggaccagat gagcagccgc gcggcctcgg ccagccccta caccccagag
       61 cacgccgcca gcgtgcccac ccactcgccc tacgcacaac ccagctccac cttcgacacc
      121 atgtcgccgg cgcctgtcat cccctccaac accgactacc ccggacccca ccactttgag
      181 gtcactttcc agcagtccag cacggccaag tcagccacct ggacgtactc cccgctcttg
      241 aagaaactct actgccagat cgccaagaca tgccccatcc agatcaaggt gtccaccccg
      301 ccacccccag gcaccgccat ccgggccatg cctgtttaca agaaagcgga gcacgtgacc
      361 gacgtcgtga aacgctgccc caaccacgag ctcgggaggg acttcaacga aggacagtct
      421 gctccagcca gccacctcat ccgcgtggaa ggcaataatc tctcgcagta tgtggatgac
      481 cctgtcaccg gcaggcagag cgtcgtggtg ccctatgagc caccacaggt ggggacggaa
      541 ttcaccacca tcctgtacaa cttcatgtgt aacagcagct gtgtaggggg catgaaccgg
      601 cggcccatcc tcatcatcat caccctggag atgcgggatg ggcaggtgct gggccgccgg
      661 tcctttgagg gccgcatctg cgcctgtcct ggccgcgacc gaaaagctga tgaggaccac
      721 taccgggagc agcaggccct gaacgagagc tccgccaaga acggggccgc cagcaagcgt
      781 gccttcaagc agagcccccc tgccgtcccc gcccttggtg ccggtgtgaa gaagcggcgg
      841 catggagacg aggacacgta ctaccttcag gtgcgaggcc gggagaactt tgagatcctg
      901 atgaagctga aagagagcct ggagctgatg gagttggtgc cgcagccact ggtggactcc
      961 tatcggcagc agcagcagct cctacagagg ccgagtcacc tacagccccc gtcctacggg
     1021 ccggtcctct cgcccatgaa caaggtgcac gggggcatga acaagctgcc ctccgtcaac
     1081 cagctggtgg gccagcctcc cccgcacagt tcggcagcta cacccaacct ggggcccgtg
     1141 ggccccggga tgctcaacaa ccatggccac gcagtgccag ccaacggcga gatgagcagc
     1201 agccacagcg cccagtccat ggtctcgggg tcccactgca ctccgccacc cccctaccac
     1261 gccgacccca gcctcgtcag ttttttaaca ggattggggt gtccaaactg catcgagtat
     1321 ttcacctccc aagggttaca gagcatttac cacctgcaga acctgaccat tgaggacctg
     1381 ggggccctga agatccccga gcagtaccgc atgaccatct ggcggggcct gcaggacctg
     1441 aagcagggcc acgactacag caccgcgcag cagctgctcc gctctagcaa cgcggccacc
     1501 atctccatcg gcggctcagg ggaactgcag cgccagcggg tcatggaggc cgtgcacttc
     1561 cgcgtgcgcc acaccatcac catccccaac cgcggcggcc caggcggcgg ccctgacgag
     1621 tgggcggact tcggcttcga cctgcccgac tgcaaggccc gcaagcagcc catcaaggag
     1681 gagttcacgg aggccgagat ccactagtaa tgaactagtc cgtaacttga aagtatttcg
     1741 atttcttggc tttatatatc ttgtggaaag gacgatgcac ctcgctcaat accaagacc
//
