LOCUS       sequence-282741-         956 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 956)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 956)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1725
FEATURES             Location/Qualifiers
     source          1..956
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tgggcagtga gaaggagcag agtccagaac cacacctgcc tgaggaaggg
       61 gaagggggta agccttggag agtggatgac tcagagggtt cttggatccc acctggggag
      121 aaggagcatg ggcaagagag cctgtcggat gaactgcaag aaactcatcc aaaaaagcca
      181 tggcagaaag tcactgtccg ggctcgagag ctaggggacc ccattgctca tccaaggcat
      241 gaggcagatg agaagccctt tatatgtgcc cagtgtggca aaaccttcaa taatacctcc
      301 aacctgagaa cacaccagcg gatccacact ggtgagaagc cttacaagtg ttctgaatgt
      361 ggcaagagct tctcgagaag ctccaaccgc atccggcacg agcggatcca cctggaagag
      421 aaacactaca aatgccccaa gtgccaggag agctttcggc ggcgctcaga cctcaccacg
      481 caccagcaag atcacctagg caagcggcca taccgctgtg acatctgtgg caagagcttc
      541 agccagagtg ccacgctagc tgtgcatcac cggacccacc tggagccagc accctacatc
      601 tgctgtgagt gtgggaagag cttcagcaac agctccagct ttggcgtgca tcaccgcacc
      661 cacacaggtg agagacctta tgagtgcact gagtgtgggc ggaccttcag cgatatctcc
      721 aactttggag cacaccagcg gacccacaga ggggagaagc cctaccggtg cactgtgtgt
      781 gggaaacact tctcccggag ctcgaatctc atccgccacc agaaaactca cttgggcgaa
      841 caggctggga aagattccag ctagtaatga actagtccgt aacttgaaag tatttcgatt
      901 tcttggcttt atatatcttg tggaaaggac gagtttagac acgtagttaa cttcta
//
