LOCUS       sequence-28275-e         797 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 797)
  AUTHORS   Ebert MS, Neilson JR, Sharp PA
  TITLE     MicroRNA sponges: competitive inhibitors of small RNAs in mammalian 
            cells.
  JOURNAL   Nat Methods. 2007 Sep . 4(9):721-6.
  PUBMED    17694064
REFERENCE   2  (bases 1 to 797)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2007 Sep . 4(9):721-6."
COMMENT     Sequence Label: U6+27-16pf
FEATURES             Location/Qualifiers
     source          1..797
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(208..225)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(208..224)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(217..239)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      366..795
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(453..472)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     663..684
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 gatttcttgg ctttatatat cttgtggaaa ggacgaaaca ccgtgctcgc ttcggcagca
       61 catatactag tcgacgcggc cgcctcgagc gccaatattt acgtgctgct acgccaatat
      121 ttacgtgctg ctagggcccg tttaaacccg ctctagagcg gacttcggtc cgctttttac
      181 taggacctgc aggcatgcaa gcttggcact ggccgtcgtt ttacaacgtc gtgactggga
      241 aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg
      301 taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga
      361 atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt
      421 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct
      481 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg
      541 atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag
      601 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa
      661 tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga
      721 tttatnangg attttgccga tttcggccta ttggtttaaa aaatgagctg atttaacaaa
      781 aatttaacgc gaattat
//
