LOCUS       sequence-282750-        1673 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1673)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1673)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1742
FEATURES             Location/Qualifiers
     source          1..1673
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggctcttcc tcagggtcta ttgacattca gggatgtggc catagaattc
       61 tctcaggagg agtggaaatg cctggaccct gctcagagga ctctatacag ggacgtgatg
      121 ctggagaatt ataggaacct ggtctccctg gatatctctt ccaaatgcat gatgaaggag
      181 ttctcgtcaa cagcacaagg caatagagaa gtgttccatg cagggacatc tcaaagacat
      241 gaaagtcatc acaatggaga tttttgtttc caggatattg ataaagatat tcatgacatt
      301 gagtttcagt ggcaagaaga tgaaagaaat ggccatgaag cactcatgac aaaaatcaaa
      361 aagttgacag gtagtacaga gcgatatgat caaaattatg ctggaaacaa gcctgttaaa
      421 tatcagcttg gattcagctt tcattcgcat ctgcctgaac tgcacatatt tcacactgaa
      481 gagaaaattg ataatcaagt tgtgaagtct atccacgatg cttccttggt ttcaacagcc
      541 caaagaattt cttgtaggcc tgaaacccat atttctaatg actatgggaa taatttcctg
      601 aattcttcat tattcacaca aaaacaggaa gtacatatga gggaaaaatc tttccaatgc
      661 aatgacagtg gcaaagccta taattgtagc tcactcttaa ggaaacatca gttaatccat
      721 ttaggagaga aacaatataa atgcgatata tgtggcaagg tctttaattc gaagcgatac
      781 gttgcacgcc atcgtagatg tcacactggt gagaaacctt acaagtgtaa tgagtgtggc
      841 aagaccttca gtcagacata ttacctaaca tgccatcgta gacttcatac tggagagaaa
      901 ccttacaaat gtgaagaatg tgacaaagct ttccatttca aatcaaagct tcaaatacat
      961 aggagaattc atactggaga gaaaccgtac aagtgtaatg agtgtggcaa gacctttagt
     1021 cagaagtcgt accttacatg ccatcgtagg cttcatactg gagagaaacc ttacaagtgt
     1081 aatgagtgtg gcaagacctt tagtcggaag tcacatttta catgccatca tagagttcat
     1141 actggagaga aaccttacaa gtgtaatgag tgtagcaaga cctttagtca caagtcatct
     1201 cttacatacc atcgtagact tcatactgaa gagaaacctt acaagtgtaa tgagtgtggc
     1261 aagaccttca atcagcagtt aacccttaac atttgtagac ttcatagtgg agagaaacct
     1321 tacaaatgtg aagaatgtga caaagcctac agtttcaaat caaaccttga aatacatcag
     1381 aaaattcata ctgaagagaa tccttacaag tgtaatgagt gtggcaagac cttcagccgg
     1441 acgtcatccc ttacatacca tcatagactt catactggac agaaacctta caaatgtgaa
     1501 gattgtgatg aagctttcag tttcaaatca aaccttgaaa gacataggag aatttatact
     1561 ggagagaaac tacacgtgta gtaatgaact agtccgtaac ttgaaagtat ttcgatttct
     1621 tggctttata tatcttgtgg aaaggacgac atccgttact aggcccgctt agc
//
