LOCUS       sequence-282753-        2258 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2258)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2258)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1768
FEATURES             Location/Qualifiers
     source          1..2258
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggctcttcc tcagggtcta ttgacattca gggacgtggc catagaattc
       61 tctcaggagg agtggaaatg cctggaccct gctcagagga ctctttacag ggatgtgatg
      121 ctggagaatt ataggaacct ggtctccctg gatatctctt ccaaatgcat gatgaagaca
      181 ttcttctcaa cagggcaagg caatacagaa gcgttccaca cagggacatt gcaaagacaa
      241 gcaagtcatc acattggaga tttttgcttc cagaaaattg agaaagacat tcatggcttc
      301 cagtttcagt ggaaagaaga tgaaacaaat gaccatgcag cacccatgac agaaatcaaa
      361 gagttgactg gtagtacagg ccaacatgat caaaggcatg ctggaaacaa gcatattaaa
      421 gatcagcttg gattaagctt tcattcgcat ctgcctgaac tgcacatatt tcagcctgaa
      481 gggaaaattg gtaatcaagt tgagaagtct atcaacaatg cttcctcagt ttcaacatcc
      541 caaagaattt gttgtaggcc caaaacccat atttctaata agtatggaaa taattccctc
      601 cattcttcat tactcacaca aaaacggaat gtacacatga gagaaaaatc tttccaatgt
      661 attgagagtg gcaaatcctt taattgtagc tcacttttaa aaaaacatca gataacccac
      721 ttagaagaga aacaatgtaa atgtgatgta tatggcaagg tatttaatca gaagcgatac
      781 cttgcatgcc atcgtagatc tcacattgat gagaaacctt acaagtgtaa tgagtgtggc
      841 aagatctttg gtcacaatac atccctcttc cttcacaagg cgcttcatac tgcagacaaa
      901 ccttatgaat gtgaagaatg tgacaaagtt ttcagtcgca aatcacacct tgaaacacat
      961 aagataattt atactggagg gaaaccatac aaatgtaagg tttgtgacaa agctttcaca
     1021 tgtaattcat acctagcaaa acatactata attcacactg gagagaaacc ttacaagtgt
     1081 aatgaatgtg gcaaggtttt taatcgactg tcaacccttg cacgccatcg taggcttcat
     1141 actggagaga aaccttatga atgtgaagaa tgtgaaaaag ttttcagtcg caaatcacat
     1201 cttgaaagac ataagaggat tcatactgga gagaaaccat acaaatgtaa ggtttgtgac
     1261 aaggcttttg catataattc atacctggca aaacatagta taattcacac tggagagaag
     1321 ccttacaagt gtaatgaatg tggcaaggtt tttaatcaac aatcaactct tgcacgccat
     1381 catagacttc atactgcaga gaaaccatac aaatgtgaag aatgtgacaa agttttcagg
     1441 tgcaaatcac accttgaaag acataggagg attcatactg gagagaaacc atacaaatgt
     1501 aaggtttgtg acaaggcttt ccggagtgat tcatgcctta cagaacatca gagagttcat
     1561 actggagaga aaccatacat gtgtaatgaa tgtggcaagg tttttagtac aaaagcaaac
     1621 cttgcatgtc atcataaact tcatactgca gagaaaccgt acaaatgtga agaatgtgag
     1681 aaagttttca gtcgcaaatc acacatggaa agacatagga ggattcatac tggagagaaa
     1741 ccgtacaaat gtaaggtttg tgacaaggct ttccggaggg attcacacct ggcacaacat
     1801 cagagagttc atactggaga gaaaccttac aagtgtaatg agtgtggcaa gaccttccgt
     1861 cagacatcat cgcttataat ccatcgtagg cttcatactg gagagaaacc ttacaagtgt
     1921 aatgagtgtg gcaagacctt cagtcagatg tcatccctcg tataccatca taggcttcat
     1981 agtggagaga aaccttacaa gtgtaatgaa tgtggcaagg tttttaatca acaagcacac
     2041 cttgcacagc atcagagagt tcatactgga gagaaacctt acaagtgtaa tgagtgtggc
     2101 aagaccttca gtcagatgtc aaaccttgta taccatcata gacttcatag tggagagaaa
     2161 ccttagtaat gaactagtcc gtaacttgaa agtatttcga tttcttggct ttatatatct
     2221 tgtggaaagg acgaccagcc gggcacgccg tttgatat
//
